1887

Abstract

Twenty-two agarolytic, aerobic, spore-forming strains were characterized taxonomically by DNA–DNA reassociation experiments, riboprint analyses, 16S rDNA sequencing and phenetic similarity analyses. Based on riboprint analyses, the strains formed eight ribogroups, six of which contained 2–6 strains and two encompassed single strains. Within the multi-strain ribogroups, similarities ranged from 91–99 %. Phylogenetic analyses of representatives of the eight groups by 16S rDNA sequence analysis showed that the strains were affiliated to the genus , but relatedness to described species was only moderate (<97·8 % sequence similarity). Published DNA–DNA similarity values for most of the agarolytic strains, supplemented with new data, supported the distinctiveness of the eight ribogroups. Intragroup DNA–DNA similarity values ranged from 80 to 104 %, while intergroup DNA–DNA similarities were <35 %. Based on genomic distinctiveness and supported by the presence of distinguishing phenotypic properties, multi-strain groups 1 and 2 are proposed as novel species, sp. nov., nom. rev. (type strain, DSM 1327=CIP 107437) and sp. nov. (type strain, DSM 1355=CIP 107436).

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2003-07-01
2019-12-08
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Cells and spores of (A) strain DSM 1327 , grown on nutrient agar (+Mn , +0.1 % urea) for 3 days at 30 °C; (B) strain DSM 1355 , grown on nutrient agar (+0.5 % glucose, +0.1 % urea) for 3 days at 28 °C. Bars, 10 µm.

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