1887

Abstract

An intracellular symbiotic bacterium was isolated from the flora of a natural clone of the black bean aphid . The strain was able to grow freely in aerobic conditions on a rich medium containing 1 % of each of the following substrates: glucose, yeast extract and casein peptone. Pure culture was achieved through the use of solid-phase culture on the same medium and the strain was designated CWBI-2.3. 16S rRNA gene sequence analysis revealed that strain CWBI-2.3 was a member of the class having high sequence similarity (>99 %) with ‘ Serratia symbiotica’, the R-type of secondary endosymbiont that is found in several aphid species. As strain CWBI-2.3 ( = LMG 25624 = DSM 23270) was the first R-type symbiont to be isolated and characterized, it was designated as the type strain of sp. nov.

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2011-09-01
2019-12-08
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