1887

Abstract

Whilst searching for a molecular method to identify the different species of and , it was observed that the OXY-1 and OXY-2 -lactamase-producing isolates displayed two distinguishable enterobacterial repetitive intergenic consensus (ERIC)-1R profiles. It was hypothesized that the two groups of chromosomal -lactamases might correspond to two groups of strains in the taxon. To confirm this hypothesis, clinical isolates and reference strains of were studied by determination of the sequence of their genes, and of a partial fragment of their 16S rRNA (387 bp) and (512 bp) genes. The sequence data were phylogenetically analysed by using the parsimony method. Four clinical isolates possessed a gene and nine possessed a gene. The mean percentage of and 16S rRNA gene identity was >99 % within each group of strains, whereas it was 96·56±0·24 % for genes and 97·80±0·22 % for 16S rRNA genes between the group of strains harbouring the gene and the group harbouring the gene. The phylogenetic tree resulting from combined analysis of the 16S rRNA and datasets showed that the isolates were monophyletic and separated into two clades; these clades included strains with either the gene or the gene. This result was supported with high bootstrap values of 97 and 99 %, respectively. Moreover, the two groups of strains displayed distinct ERIC-1R profiles, with bands characteristic of each profile. Thus, the chromosomal gene sequence is able to delineate not only two groups of -lactamases in , but also two clades in the taxon, in a manner similar to the sequence of housekeeping genes. These results suggest that should be divided into two genetic groups, group OXY-1 represented by strain SL781 (=CIP 104963) and group OXY-2 by strain SL911 (=CIP 106098).

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2003-05-01
2024-12-12
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