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Abstract

A Gram-positive-staining, aerobic, moderately halophilic bacterium, designated strain Y32, was isolated from subsurface soil of the Sanwei salt field, Yancheng, Jiangsu province, South-east China. The cell-wall peptidoglycan type of strain Y32 was -diaminopimelic acid. The menaquinone was menaquinone-7 (MK-7). The major fatty acids were anteiso-C and iso-C and the DNA G+C content of strain Y32 was 40.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Y32 was closely related to the type strains of the genus . The 16S rRNA gene sequence similarities of strain Y32 with the type strains of species of the genus were 97.8 % ( KCTC 3917), 96.9 % ( BH030062), 96.8 % ( JSM 072002) and 96.0 % ( JSM 076056). DNA–DNA relatedness between strain Y32 and . KCTC 3917 was 42 %. On the basis of phenotypic, chemotaxonomic and phylogenetic evidences, strain Y32 was found to be affiliated to the genus , but was clearly differentiated from other members of this genus. Strain Y32 represents a novel member of the genus, for which the name sp. nov. is proposed. The type strain is Y32 ( = CGMCC 1.10680 = CCTCC AB209311 = NRRL B-59408).

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2011-08-01
2019-10-23
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vol. , part 8, pp. 1906 - 1911

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Transmission electron micrograph of cells of strain Y32 (1 day on MA plus 5% NaCl).

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Maximum-likelihood (a) and maximum-parsimony (b) trees showing the position of strain Y32 among related taxa inferred from 16S rRNA gene sequences. Bootstrap percentages based on 1000 replications are shown at nodes. [ PDF] 819 KB

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