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Abstract

A polyphasic taxonomic study was performed on a strain of an unknown Gram-negative, non-motile, saccharolytic, facultatively anaerobic bacterium, strain OCF 7, isolated from anoxic freshwater sediment. The strain grew optimally at 22 °C and pH 7.5, and was able to grow under strictly anaerobic conditions. Major fermentation products from glucose metabolism were formate, acetate, ethanol and lactate. Comparative 16S rRNA gene sequence analysis indicated that strain OCF 7 was phylogenetically related to the type strain of (97.2 % similarity) within the family of the . However, OCF 7 did not produce toluene from phenylacetate, phenylalanine, phenoxyacetate, phenylsuccinate or phenylbutyrate in the presence of glucose. Phenol was not produced from tyrosine or phenoxyacetate in the presence of glucose. Dominant fatty acids of this micro-organism included C, Cω7 and Cω7 (and/or iso-C 2-OH). Major polar lipids were phosphatidylglycerol and phosphatidylethanolamine, and the respiratory quinone was menaquinone MK-8. The genomic DNA G+C content of strain OCF 7 was 52.1 mol%. Based on phylogenetic and phenotypic evidence, strain OCF 7 should be classified as a representative of a novel species of , for which the name sp. nov. is proposed; the type strain is OCF 7 ( = DSM 22975  = ATCC BAA-1908). An emended description of the genus is also given.

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2011-11-01
2019-10-16
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Supplements

vol. , part 11, pp. 2659 - 2663

Substrate utilization data from the Biolog GN2 MicroPlate useful in distinguishing between DSM 9187 and strain OCF 7 when grown aerobically.

Cellular fatty acid composition of strain OCF 7 grown on TSB and basal toluene production (TP) medium.

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