1887

Abstract

A Gram-stain-positive, rod-shaped bacterium, designated strain NH3, was isolated from a sediment sample from the South China Sea and was subjected to a polyphasic taxonomic study. The isolate grew optimally at 37 °C and pH 9. Strain NH3 had cell-wall peptidoglycan based on -diaminopimelic acid and MK-7 as the predominant menaquinone. The cellular fatty acid profile included significant amounts of iso-C and iso-C. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content of strain NH3 was 40.3 mol%. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain NH3 was a member of rRNA group 6 of the genus , which includes alkalitolerant, alkaliphilic and halotolerant species. The closest phylogenetic relatives were 1139 (96.82 % 16S rRNA gene sequence similarity), DSM 8715 (96.76 %), Kh10-101 (96.76 %) and MS 6 (96.47 %). Strain NH3 could be distinguished from these phylogenetically close neighbours based on a number of phenotypic properties. On the basis of phenotypic and chemotaxonomic characteristics and phylogenetic data, we conclude that strain NH3 ( = CGMCC 1.10116  = JCM 16507) merits classification as the type strain of a novel species, for which the name sp. nov. is proposed.

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2011-05-01
2019-10-20
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Photomicrograph of sporangia and vegetative cells of strain NH3 viewed by phase-contrast microscopy. Bar, 3 µm.

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Two-dimensional TLC of polar lipids of strain NH3 stained with ninhydrin, molybdenum blue and α-naphthol. DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; PN, unknown aminophospholipid; GL, unknown glycolipid.

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Fatty acid compositions of strain NH3 and JCM 9157 . [ PDF] (43 KB)

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