1887

Abstract

Two Gram-positive or Gram-variable, endospore-forming, moderately halophilic rods (strains TF-11 and TF-12) were isolated from a tidal flat of the Yellow Sea in Korea and were subjected to a polyphasic taxonomic study. Strains TF-11 and TF-12 had cell-wall peptidoglycan based on -diaminopimelic acid. The predominant menaquinone found in the two strains was MK-7. The cellular fatty acid profiles of both strains contained large amounts of branched and saturated fatty acids. The major fatty acids were anteiso-C and iso-C. The DNA G+C contents of strains TF-11 and TF-12 were respectively 49 and 38 mol%. Phylogenetic analysis based on 16S rDNA sequences showed that strains TF-11 and TF-12 fell within the radiation of the cluster comprising species. The level of 16S rDNA sequence similarity between strains TF-11 and TF-12 was 98·3 %. Strains TF-11 and TF-12 exhibited levels of 16S rDNA sequence similarity of less than 96·0 and 96·3 %, respectively, to species. The mean level of DNA–DNA relatedness between the two strains was approximately 7 %. On the basis of phenotypic and phylogenetic data and genomic distinctiveness, strains TF-11 and TF-12 should be placed in the genus as two distinct novel species, for which the names sp. nov. (type strain TF-11=KCCM 41588=JCM 11544) and sp. nov. (type strain TF-12=KCCM 41589=JCM 11545) are proposed.

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2003-09-01
2019-12-12
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vol. , part 5, pp. 1297 - 1303

Neighbour-joining tree showing the phylogenetic positions of strains TF-11 and TF-12 and an expanded selection of representatives of related taxa based on 16S rDNA sequences. Bar, 0·01 substitutions per nucleotide position. Bootstrap values (expressed as percentages of 1000 replications) greater than 50 % are shown at branch-points. [PDF](101 KB)



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