1887

Abstract

A facultatively anaerobic, prosthecate bacterium, strain Mfc52, was isolated from a microbial fuel cell inoculated with soil and fed with cellulose as the sole fuel. Cells were Gram-negative, non-spore-forming, straight or slightly curved rods, and some of them had one or two polar prosthecae (stalks). Cells reproduced by binary fission or by budding from mother cells having prosthecae. Strain Mfc52 fermented various sugars and produced lactate, acetate and fumarate. Ferric iron, nitrate, oxygen and fumarate served as electron acceptors, while sulfate and malate did not. Nitrate was reduced to nitrite. The DNA G+C content was 64.7 mol%. On the basis of 16S rRNA gene sequence phylogeny, strain Mfc52 was affiliated with the genus in the class and most closely related to with a sequence similarity of 97 %. Based on these physiological and phylogenetic characteristics, the name sp. nov. is proposed; the type strain is Mfc52 ( = JCM 15089  = KCTC 5806).

Funding
This study was supported by the:
  • Japan Society for Promotion of Science (JSPS)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.023580-0
2011-08-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/8/1781.html?itemId=/content/journal/ijsem/10.1099/ijs.0.023580-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. 1990; Basic local alignment search tool. J Mol Biol 215:403–410[PubMed] [CrossRef]
    [Google Scholar]
  2. Beveridge T. J., Popkin T. J., Cole R. M. 1994; Electron microscopy. In Methods for General and Molecular Bacteriology pp. 42–71 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  3. Bowman J. P., Sly L. I., Nichols P. D., Hayward A. C. 1993; Revised taxonomy of the methanotrophs: description of Methylobacter gen. nov., emendation of Methylococcus, validation of Methylosinus and Methylocystis species, and a proposal that the family Methylococcaceae includes only the group I methanotrophs. Int J Syst Bacteriol 43:735–753 [View Article]
    [Google Scholar]
  4. Ishii S., Kosaka T., Hori K., Hotta Y., Watanabe K. 2005; Coaggregation facilitates interspecies hydrogen transfer between Pelotomaculum thermopropionicum and Methanothermobacter thermautotrophicus . Appl Environ Microbiol 71:7838–7845 [View Article][PubMed]
    [Google Scholar]
  5. Ishii S., Shimoyama T., Hotta Y., Watanabe K. 2008a; Characterization of a filamentous biofilm community established in a cellulose-fed microbial fuel cell. BMC Microbiol 8:6 [View Article][PubMed]
    [Google Scholar]
  6. Ishii S., Hotta Y., Watanabe K. 2008b; Methanogenesis versus electrogenesis: morphological and phylogenetic comparisons of microbial communities. Biosci Biotechnol Biochem 72:286–294 [View Article][PubMed]
    [Google Scholar]
  7. Kaku N., Yonezawa N., Kodama Y., Watanabe K. 2008; Plant/microbe cooperation for electricity generation in a rice paddy field. Appl Microbiol Biotechnol 79:43–49 [View Article][PubMed]
    [Google Scholar]
  8. Katayama-Fujimura Y., Komatsu Y., Kuraishi H., Kaneko T. 1984; Estimation of DNA base composition by high performance liquid chromatography of its nuclease P1 hydrolysate. Agric Biol Chem 48:3169–3172 [CrossRef]
    [Google Scholar]
  9. Kodama Y., Watanabe K. 2003; Isolation and characterization of a sulfur-oxidizing chemolithotroph growing on crude oil under anaerobic conditions. Appl Environ Microbiol 69:107–112 [View Article][PubMed]
    [Google Scholar]
  10. Kodama Y., Watanabe K. 2008; An electricity-generating prosthecate bacterium strain Mfc52 isolated from a microbial fuel cell. FEMS Microbiol Lett 288:55–61 [View Article][PubMed]
    [Google Scholar]
  11. Logan B. E., Hamelers B., Rozendal R., Schröder U., Keller J., Freguia S., Aelterman P., Verstraete W., Rabaey K. 2006; Microbial fuel cells: methodology and technology. Environ Sci Technol 40:5181–5192 [View Article][PubMed]
    [Google Scholar]
  12. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  13. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–655 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  14. Stookey L. L. 1970; Ferrozine – a new spectrophotometric reagent for iron. Anal Chem 42:779–781 [View Article]
    [Google Scholar]
  15. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
    [Google Scholar]
  16. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
    [Google Scholar]
  17. Ueki A., Kodama Y., Kaku N., Shiromura T., Satoh A., Watanabe K., Ueki K. 2010; Rhizomicrobium palustre gen. nov., sp. nov., a facultatively anaerobic, fermentative stalked bacterium in the class Alphaproteobacteria isolated from rice plant roots. J Gen Appl Microbiol 56:193–203 [View Article][PubMed]
    [Google Scholar]
  18. Watanabe K. 2008; Recent developments in microbial fuel cell technologies for sustainable bioenergy. J Biosci Bioeng 106:528–536 [View Article][PubMed]
    [Google Scholar]
  19. Xie C.-H., Yokota A. 2005; Pleomorphomonas oryzae gen. nov., sp. nov., a nitrogen-fixing bacterium isolated from paddy soil of Oryza sativa . Int J Syst Evol Microbiol 55:1233–1237 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.023580-0
Loading
/content/journal/ijsem/10.1099/ijs.0.023580-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error