1887

Abstract

A catalase-negative actinomycete, strain YIM 21741, was isolated from a soil sample collected from a primeval forest at Xishuangbanna, Yunnan Province, China. Analysis of 16S rDNA showed the strain to be related to members of the genus , with which it also shares morphological characteristics, e.g. branching hyphae breaking into diphtheroid and rod-like, irregular, non-motile fragments and a peptidoglycan containing the diagnostic amino acid 2,4-diamino--butyric acid. Whole-cell hydrolysates of strain YIM 21741 contained rhamnose and small quantities of glucose, galactose and mannose. The major menaquinone was MK-12, while MK-13 and MK-12 were minor components. Diagnostic phospholipids were phosphatidylglycerol and diphosphatidylglycerol. The G+C content of the DNA was 72·8 mol%. Physiological and biochemical characteristics reveal strain YIM 21741 to be different from all validly described species of the genus . As DNA–DNA similarity values between this isolate and the phylogenetically neighbouring type strains of and are only moderate, the novel species sp. nov. is proposed with strain YIM 21741 (=CCTCC 001012 =AS 4.1717 =DSM 14598) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.02350-0
2003-01-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/53/1/ijs530303.html?itemId=/content/journal/ijsem/10.1099/ijs.0.02350-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  2. Becker, B., Gordon, R. E., Lechevalier, H. A. & Lechevalier, M. P. ( 1964; ). Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole cell hydrolysates. Appl Microbiol 12, 421–423.
    [Google Scholar]
  3. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M. ( 1977; ). A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef]
    [Google Scholar]
  4. Collins, M. D. & Jones, D. ( 1980; ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48, 459–470.[CrossRef]
    [Google Scholar]
  5. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  6. Cui, X.-L., Mao, P.-H., Tseng, M., Li, W.-J., Zhang, L.-P., Xu, L.-H. & Jiang, C.-L. ( 2001; ). Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51, 357–363.
    [Google Scholar]
  7. De Soete, G. ( 1983; ). A least squares algorithm for fitting additive trees to proximity data. Psychometrika 48, 621–626.[CrossRef]
    [Google Scholar]
  8. Escara, J. F. & Hutton, J. R. ( 1980; ). Thermal stability and renaturation of DNA in dimethyl sulfoxide solutions: acceleration of the renaturation rate. Biopolymers 19, 1315–1327.[CrossRef]
    [Google Scholar]
  9. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  10. Felsenstein, J. ( 1985; ). Conference limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–789.[CrossRef]
    [Google Scholar]
  11. Gledhill, W. E. & Casida, L. E. ( 1969; ). Predominant catalase negative soil bacteria. III. Agromyces gen. nov., microorganisms intermediary to Actinomyces and Nocardia. Appl Microbiol 18, 340–349.
    [Google Scholar]
  12. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  13. Hayakawa, M. & Nonomura, H. ( 1987; ). Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. J Ferment Technol 65, 501–509.[CrossRef]
    [Google Scholar]
  14. Higgins, D. G., Bleasby, A. J. & Fuchs, R. ( 1992; ). clustal v: improved software for multiple sequence alignment. Comput Appl Biosci 8, 189–191.
    [Google Scholar]
  15. Hopwood, D. A., Bill, M. J., Charter, K. F. & 7 other authors ( 1985; ). Genetic Manipulation of Streptomycetes: a Laboratory Manual. Norwich: John Innes Foundation.
  16. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  17. Jahnke, K.-D. ( 1992; ). BASIC computer program for evaluation of spectroscopic DNA renaturation data from Gilford System 2600 spectrophotometer on a PC/XT/AT type personal computer. J Microbiol Methods 15, 61–73.[CrossRef]
    [Google Scholar]
  18. Kelly, K. L. ( 1964; ). Inter-Society Color Council–National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office.
  19. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  20. Lechevalier, M. P. & Lechevalier, H. A. ( 1980; ). The chemotaxonomy of actinomycetes. In Actinomycete Taxonomy, pp. 227–291. Edited by X. Dietz & Y. Thayer. Arlington, VA: Society for Industrial Microbiology.
  21. Mandel, M. & Marmur, J. ( 1968; ). Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B, 195–206.
    [Google Scholar]
  22. Mikami, H. & Ishida, Y. ( 1983; ). Post-column fluorometric detection of reducing sugars in high-performance liquid chromatography using arginine. Bunseki Kagaku 32, E207–E210.[CrossRef]
    [Google Scholar]
  23. Minnikin, D. E., Collins, M. D. & Goodfellow, M. ( 1979; ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Microbiol 47, 87–95.
    [Google Scholar]
  24. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  25. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  26. Suzuki, K., Sasaki, J., Uramoto, M., Nakase, T. & Komagata, K. ( 1996; ). Agromyces mediolanus sp. nov., nom. rev., comb. nov., a species for “Corynebacterium mediolanum” Mamoli 1939 and for some aniline-assimilating bacteria which contain 2,4-diaminobutyric acid in the cell wall peptidoglycan. Int J Syst Bacteriol 46, 88–93.[CrossRef]
    [Google Scholar]
  27. Takahashi, Y. & Egusa, H. ( 1992; ). Quantitative analysis for madurose and other sugars in a small amount of actinomycete whole cells by gas-liquid chromatography. Actinomycetologica 6, 69–78.[CrossRef]
    [Google Scholar]
  28. Takeuchi, M. & Hatano, K. ( 1998; ). Gordonia rhizosphera sp. nov. isolated from the mangrove rhizosphere. Int J Syst Bacteriol 48, 907–912.[CrossRef]
    [Google Scholar]
  29. Takeuchi, M. & Hatano, K. ( 2001; ). Agromyces luteolus sp. nov., Agromyces rhizospherae sp. nov. and Agromyces bracchium sp. nov., from the mangrove rhizosphere. Int J Syst Evol Microbiol 51, 1529–1537.
    [Google Scholar]
  30. Williams, S. T., Goodfellow, M. & Alderson, G. ( 1989; ). Genus Streptomyces Waksman and Henrici 1943. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2452–2492. Edited by S. T. Williams, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  31. Zgurskaya, H. I., Evtushenko, L. I., Akimov, V. N., Voyevoda, H. V., Dobrovolskaya, T. G., Lysak, L. V. & Kalakoutskii, L. V. ( 1992; ). Emended description of the genus Agromyces and description of Agromyces cerinus subsp. cerinus sp. nov., subsp. nov., Agromyces cerinus subsp. nitratus sp. nov., subsp. nov., Agromyces fucosus subsp. fucosus sp. nov., subsp. nov., and Agromyces fucosus subsp. hippuratus sp. nov., subsp. nov. Int J Syst Bacteriol 42, 635–641.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.02350-0
Loading
/content/journal/ijsem/10.1099/ijs.0.02350-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error