A facultatively anaerobic, Gram-positive, coccoid, non-spore-forming, non-motile, catalase-negative bacterium was isolated from the uropygial (preen) gland of wild Red-billed Woodhoopoes () and designated strain JLB-1. Physiological and biochemical testing suggested that this homofermentative, lactic-acid-producing bacterium could belong to the genus or the genus . Comparison of the 16S rRNA gene sequence of strain JLB-1 with other 16S rDNA sequences in the GenBank database by analysis showed that its closest relatives are , and . Strain JLB-1 may be differentiated from these species by the fact that it does not produce acid from lactose, -mannitol, (+)-melezitose or -sorbitol. Furthermore, it does not hydrolyse arginine or hippurate and cannot grow in the presence of 6·5 % NaCl or 40 % bile. It differs from the streptococci in that it does not lyse erythrocytes. Strain JLB-1 is a novel member of the enterococci, for which the name sp. nov. is proposed. The type strain is JLB-1 (=ATCC BAA-412=DSM 14726).


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  1. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  2. Cooper, J. E. ( 1990; ). Birds and zoonoses. Ibis 132, 181–191.
    [Google Scholar]
  3. Cooper, J. E. ( 1993; ). Historical survey of disease in birds. J Zoo Wildl Med 24, 256–264.
    [Google Scholar]
  4. Cooper, J. E., Needham, J. R. & Fox, N. C. ( 1986; ). Bacteriological, haematological and clinical studies on the Mauritius Kestrel (Falco punctatus). Avian Pathol 15, 349–359.[CrossRef]
    [Google Scholar]
  5. Cornelissen, J. M., van den Brink, M. E., Bakker, M. H. & Koopman, J. P. ( 1991; ). Cloacal microflora of healthy hornbills, toucans and aracaris. Tijdschr Diergeneeskd 116 (Suppl. 1), 84S–85S.
    [Google Scholar]
  6. Devriese, L. A., Dutta, G. N., Farrow, J. A. E., Van De Kerckhove, A. & Phillips, B. A. ( 1983; ). Streptococcus cecorum, a new species isolated from chickens. Int J Syst Bacteriol 33, 772–776.[CrossRef]
    [Google Scholar]
  7. Devriese, L. A., Ceyssens, K., Rodrigues, U. M. & Collins, M. D. ( 1990; ). Enterococcus columbae, a species from pigeon intestines. FEMS Microbiol Lett 71, 247–252.[CrossRef]
    [Google Scholar]
  8. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef]
    [Google Scholar]
  9. Hardie, J. M. ( 1986; ). Genus Streptococcus Rosenbach 1884, 22AL. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1043–1071. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  10. Hubálek, Z. ( 1976; ). Interspecific affinity among keratinolytic fungi associated with birds. Folia Parasitol 23, 267–272.
    [Google Scholar]
  11. Hubálek, Z., Juřicová, Z. & Halouzka, J. ( 1995; ). A survey of free-living birds as hosts and ‘lessors' of microbial pathogens. Folia Zool 44, 1–11.
    [Google Scholar]
  12. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: Molecular Evolutionary Genetics Analysis software, Arizona State University, Tempe, AZ, USA (http://www.megasoftware.net).
  13. Law-Brown, J. ( 2001; ). Chemical defence in the Red-billed Woodhoopoe, Phoeniculus purpureus. MSc thesis, University of Cape Town.
  14. Palleroni, N. J. ( 1984; ). Family I. Pseudomonadaceae Winslow, Broadhurst, Buchanan, Krumwiede, Rogers and Smith 1917, 555AL. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 141–199. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  15. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  16. Schleifer, K. H. & Kilpper-Bälz, R. ( 1984; ). Transfer of Streptococcus faecalis and Streptococcus faecium to the genus Enterococcus nom. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov. Int J Syst Bacteriol 34, 31–34.[CrossRef]
    [Google Scholar]
  17. Singleton, D. R. & Given Harper, R. ( 1998; ). Bacteria in old house wren nests. J Field Ornithol 69, 71–74.
    [Google Scholar]
  18. Summanen, P., Baron, E. J. & Citron, D. M. ( 1993; ). Wadsworth Anaerobic Bacteriology Manual, 5th edn, pp. 108–109. Belmont, CA: Star Publishing.
  19. Vancanneyt, M., Snauwaert, C., Cleenwerck, I. & 8 other authors ( 2001; ). Enterococcus villorum sp. nov., an enteroadherent bacterium associated with diarrhoea in piglets. Int J Syst Evol Microbiol 51, 393–400.
    [Google Scholar]
  20. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J. ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703.
    [Google Scholar]


vol. , part 3, pp. 683 - 685

Unrooted phylogenetic tree based on almost-complete 16S rRNA gene sequences

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