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Abstract

A bacterial strain, designated SW-3, was isolated from the reef-building coral , from Southern Taiwan, and was characterized by using a polyphasic taxonomic approach. Cells of strain SW-3 were Gram-reaction-negative, aerobic, rod-shaped and motile by means of monopolar flagella and formed cream–white colonies. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain SW-3 belonged to the genus and its most closely related neighbours were B7, DD-R11, KKL-A5, DSM 413 and R-1512, with sequence similarities of 96.8, 96.6, 96.1 , 95.8 and 94.9 %, respectively. Strain SW-3 exhibited optimal growth at 25–30 °C and pH 9–10 and in 3–5 % (w/v) NaCl. The predominant cellular fatty acid was summed feature 8 (Cω7 and/or Cω6; 75 %). The major respiratory quinone was Q-10. The polar lipid profile consisted of a mixture of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and several uncharacterized polar lipids. The DNA G+C content of strain SW-3 was 63.7 mol%. The results of physiological and biochemical tests allowed clear phenotypic differentiation of this isolate from recognized species of the genus . It is evident from the genotypic, phenotypic and chemotaxonomic data that strain SW-3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SW-3 ( = LMG 25205  = BCRC 17967).

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2011-05-01
2020-01-19
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References

  1. Berry A. , Janssens D. , Hümbelin M. , Jore J. P. M. , Hoste B. , Cleenwerck I. , Vancanneyt M. , Bretzel W. , Mayer A. F. et al. ( 2003; ). Paracoccus zeaxanthinifaciens sp. nov., a zeaxanthin-producing bacterium. . Int J Syst Evol Microbiol 53:, 231–238. [CrossRef] [PubMed]
    [Google Scholar]
  2. Chen W. M. , Laevens S. , Lee T. M. , Coenye T. , De Vos P. , Mergeay M. , Vandamme P. . ( 2001; ). Ralstonia taiwanensis sp. nov., isolated from root nodules of Mimosa species and sputum of a cystic fibrosis patient. . Int J Syst Evol Microbiol 51:, 1729–1735.[PubMed] [CrossRef]
    [Google Scholar]
  3. Chun J. , Lee J.-H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  4. Collins M. D. . ( 1985; ). Analysis of isoprenoid quinones. . Methods Microbiol 18:, 329–366. [CrossRef]
    [Google Scholar]
  5. Daneshvar M. I. , Hollis D. G. , Weyant R. S. , Steigerwalt A. G. , Whitney A. M. , Douglas M. P. , Macgregor J. P. , Jordan J. G. , Mayer L. W. et al. ( 2003; ). Paracoccus yeeii sp. nov. (formerly CDC group EO-2), a novel bacterial species associated with human infection. . J Clin Microbiol 41:, 1289–1294. [CrossRef] [PubMed]
    [Google Scholar]
  6. Davis D. H. , Doudoroff M. , Stanier R. Y. , Mandel M. . ( 1969; ). Proposal to reject the genus Hydrogenomonas: taxonomic implications. . Int J Syst Bacteriol 19:, 375–390. [CrossRef]
    [Google Scholar]
  7. Deng Z. S. , Zhao L. F. , Xu L. , Kong Z. Y. , Zhao P. , Qin W. , Chang J. L. , Wei G. H. . ( 2010; ). Paracoccus sphaerophysae sp. nov., a siderophore-producing, endophytic bacterium isolated from root nodules of Sphaerophysa salsula . . Int J Syst Evol Microbiol 61:, 665–669. [CrossRef] [PubMed]
    [Google Scholar]
  8. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  9. Felsenstein, J. (1993). phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  10. Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  11. Hall T. A. . ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. . Nucleic Acids Symp Ser 41:, 95–98.
    [Google Scholar]
  12. Hosoya S. , Adachi K. , Kasai H. . ( 2009; ). Thalassomonas actiniarum sp. nov. and Thalassomonas haliotis sp. nov., isolated from marine animals. . Int J Syst Evol Microbiol 59:, 686–690. [CrossRef] [PubMed]
    [Google Scholar]
  13. Hsu S. C. , Lockwood J. L. . ( 1975; ). Powdered chitin agar as a selective medium for enumeration of actinomycetes in water and soil. . Appl Microbiol 29:, 422–426.[PubMed]
    [Google Scholar]
  14. Katayama Y. , Hiraishi A. , Kuraishi H. . ( 1995; ). Paracoccus thiocyanatus sp. nov., a new species of thiocyanate-utilizing facultative chemolithotroph, and transfer of Thiobacillus versutus to the genus Paracoccus as Paracoccus versutus comb. nov. with emendation of the genus. . Microbiology 141:, 1469–1477. [CrossRef] [PubMed]
    [Google Scholar]
  15. Khan S. T. , Takaichi S. , Harayama S. . ( 2008; ). Paracoccus marinus sp. nov., an adonixanthin diglucoside-producing bacterium isolated from coastal seawater in Tokyo Bay. . Int J Syst Evol Microbiol 58:, 383–386. [CrossRef] [PubMed]
    [Google Scholar]
  16. Kim B. Y. , Weon H. Y. , Yoo S. H. , Kwon S. W. , Cho Y. H. , Stackebrandt E. , Go S. J. . ( 2006; ). Paracoccus homiensis sp. nov., isolated from a sea-sand sample. . Int J Syst Evol Microbiol 56:, 2387–2390. [CrossRef] [PubMed]
    [Google Scholar]
  17. Kim Y. O. , Kong H. J. , Park S. , Kang S. J. , Kim K. K. , Moon D. Y. , Oh T. K. , Yoon J. H. . ( 2010; ). Paracoccus fistulariae sp. nov., a lipolytic bacterium isolated from bluespotted cornetfish Fistularia commeronii . . Int J Syst Evol Microbiol 60:, 2908–2912. [CrossRef]
    [Google Scholar]
  18. Kimura M. . ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge:: Cambridge University Press;. [CrossRef]
    [Google Scholar]
  19. Kluge A. G. , Farris F. S. . ( 1969; ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18:, 1–32. [CrossRef]
    [Google Scholar]
  20. Kumar S. , Tamura K. , Nei M. . ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. . Brief Bioinform 5:, 150–163. [CrossRef] [PubMed]
    [Google Scholar]
  21. La H. J. , Im W.-T. , Ten L. N. , Kang M. S. , Shin D. Y. , Lee S. T. . ( 2005; ). Paracoccus koreensis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket (UASB) reactor. . Int J Syst Evol Microbiol 55:, 1657–1660. [CrossRef] [PubMed]
    [Google Scholar]
  22. Lechevalier M. P. , de Bikvre C. , Lechevalier H. A. . ( 1977; ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. . Biochem Syst Ecol 5:, 249–260. [CrossRef]
    [Google Scholar]
  23. Liu X.-Y. , Wang B.-J. , Jiang C.-Y. , Liu S.-J. . ( 2006; ). Paracoccus sulfuroxidans sp. nov., a sulfur oxidizer from activated sludge. . Int J Syst Evol Microbiol 56:, 2693–2695. [CrossRef] [PubMed]
    [Google Scholar]
  24. Liu Z.-P. , Wang B.-J. , Liu X.-Y. , Dai X. , Liu Y.-H. , Liu S.-J. . ( 2008; ). Paracoccus halophilus sp. nov., isolated from marine sediment of the South China Sea, China, and emended description of genus Paracoccus Davis 1969. . Int J Syst Evol Microbiol 58:, 257–261. [CrossRef] [PubMed]
    [Google Scholar]
  25. Lyman J. , Fleming R. H. . ( 1940; ). Composition of sea water. . J Mar Res 3:, 134–146.
    [Google Scholar]
  26. MacFaddin J. F. . ( 2000; ). Biochemical Tests for the Identification of Medical Bacteria, , 3rd edn.. Baltimore, MD:: Williams & Wilkins;.
    [Google Scholar]
  27. Maidak B. L. , Cole J. R. , Lilburn T. G. , Parker C. T. Jr , Saxman P. R. , Farris R. J. , Garrity G. M. , Olsen G. J. , Schmidt T. M. , Tiedje J. M. . ( 2001; ). The RDP-II (Ribosomal Database Project). . Nucleic Acids Res 29:, 173–174. [CrossRef] [PubMed]
    [Google Scholar]
  28. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  29. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal K. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  30. Nokhal T. H. , Schlegel H. G. . ( 1983; ). Taxonomic study of Paracoccus denitrificans . . Int J Syst Bacteriol 33:, 26–37. [CrossRef]
    [Google Scholar]
  31. Powers E. M. . ( 1995; ). Efficacy of the Ryu nonstaining KOH technique for rapidly determining Gram reactions of food-borne and waterborne bacteria and yeasts. . Appl Environ Microbiol 61:, 3756–3758.[PubMed]
    [Google Scholar]
  32. Roh S. W. , Nam Y. D. , Chang H. W. , Kim K. H. , Kim M. S. , Shin K. S. , Yoon J. H. , Oh H. M. , Bae J. W. . ( 2009; ). Paracoccus aestuarii sp. nov., isolated from tidal flat sediment. . Int J Syst Evol Microbiol 59:, 790–794. [CrossRef] [PubMed]
    [Google Scholar]
  33. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  34. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  35. Siller H. , Rainey F. A. , Stackebrandt E. , Winter J. . ( 1996; ). Isolation and characterization of a new Gram-negative, acetone-degrading, nitrate-reducing bacterium from soil, Paracoccus solventivorans sp. nov.. Int J Syst Bacteriol 46:, 1125–1130. [CrossRef] [PubMed]
    [Google Scholar]
  36. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef] [PubMed]
    [Google Scholar]
  37. Tsubokura A. , Yoneda H. , Mizuta H. . ( 1999; ). Paracoccus carotinifaciens sp. nov., a new aerobic Gram-negative astaxanthin-producing bacterium. . Int J Syst Bacteriol 49:, 277–282. [CrossRef] [PubMed]
    [Google Scholar]
  38. Wallet F. , Blondiaux N. , Foy C. L. , Loïez C. , Armand S. , Pagniez D. , Courcol R. J. . ( 2010; ). Paracoccus yeei: a new unusual opportunistic bacterium in ambulatory peritoneal dialysis. . Int J Infect Dis 14:, e173–e174. [CrossRef] [PubMed]
    [Google Scholar]
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vol. , part 5, pp. 1138 - 1143

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GN2 microplate oxidation data that distinguish strain SW-3 from other species of the genus . [PDF](78kb)

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