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Abstract

A synthetic pyrethroid (SP)-degrading bacterial strain, designated JZ-1, was isolated from activated sludge of a SP-manufacturing wastewater treatment facility and studied using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JZ-1 belonged to the genus , showing highest sequence similarities to DSM 21829 (98.6 %), JCM 10874 (98.5 %), DSM 21299 (97.4 %) and CCM 7431 (96.9 %). The polar lipid pattern, the presence of spermidine and ubiquinone Q-10, the predominance of the cellular fatty acids Cω7, C cyclo ω8, 11 methyl Cω7, C and C 2-OH, and the G+C content of the genomic DNA also supported the affiliation of the strain with the genus . Strain JZ-1 showed low DNA–DNA relatedness values with DSM 21829 (30.2 %), JCM 10874 (23.3 %), DSM 21299 (10.9 %) and CCM 7431 (7.9 %). Based on its phylogenetic position and its phenotypic and genotypic properties, strain JZ-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JZ-1 ( = CGMCC 1.7748  = DSM 21828).

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2011-08-01
2020-01-24
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vol. , part 8, pp. 1776 - 1780

Degradation of different synthetic pyrethroids over time by strain JZ-1 in mineral salts medium.

Two-dimensional thin-layer chromatography of polar lipids of strain JZ-1 .

Transmission electron micrograph of a negatively stained cell of strain JZ-1 , characterized by being short rod-shaped, non-spore-forming and having no flagellum.

Cellular fatty acid contents of strain JZ-1 and closely related species of the genus .

Differential phenotypic characteristics of strain JZ-1 and the other known species of the genus .

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