Skip to content
1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 61, Issue 8

sp. nov., a synthetic pyrethroid (SP)-degrading bacterium isolated from activated sludge in an SP-manufacturing wastewater treatment facility Free

Abstract

A synthetic pyrethroid (SP)-degrading bacterial strain, designated JZ-1, was isolated from activated sludge of a SP-manufacturing wastewater treatment facility and studied using a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JZ-1 belonged to the genus , showing highest sequence similarities to DSM 21829 (98.6 %), JCM 10874 (98.5 %), DSM 21299 (97.4 %) and CCM 7431 (96.9 %). The polar lipid pattern, the presence of spermidine and ubiquinone Q-10, the predominance of the cellular fatty acids Cω7, C cyclo ω8, 11 methyl Cω7, C and C 2-OH, and the G+C content of the genomic DNA also supported the affiliation of the strain with the genus . Strain JZ-1 showed low DNA–DNA relatedness values with DSM 21829 (30.2 %), JCM 10874 (23.3 %), DSM 21299 (10.9 %) and CCM 7431 (7.9 %). Based on its phylogenetic position and its phenotypic and genotypic properties, strain JZ-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JZ-1 ( = CGMCC 1.7748  = DSM 21828).

  • Published Online:
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 30970099)
  • National High Technology Research and Development Program of China (Award 2006AA10Z402)
  • Natural Science Foundation of Jiangsu Province, China (Award BK2008331)
© 2011 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.023309-0
2011-08-01
2025-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/8/1776.html?itemId=/content/journal/ijsem/10.1099/ijs.0.023309-0&mimeType=html&fmt=ahah

References

  1. Buck J. D. 1982; Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993[PubMed]
     [Google Scholar]
  2. Busse J., Auling G. 1988; Polyamine pattern as a chemotaxonomic marker within the Proteobacteria . Syst Appl Microbiol 11:1–8 [CrossRef]
     [Google Scholar]
  3. Busse H. J., Bunka S., Hensel A., Lubitz W. 1997; Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Bacteriol 47:698–708 [View Article]
     [Google Scholar]
  4. Busse H. J., Kämpfer P., Denner E. B. M. 1999; Chemotaxonomic characterisation of Sphingomonas . J Ind Microbiol Biotechnol 23:242–251 [View Article][PubMed]
     [Google Scholar]
  5. Chun J., Lee J.-H., Jung Y., Kim M., Kim S., Kim B. K., Lim Y. W. 2007; EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261 [View Article][PubMed]
     [Google Scholar]
  6. Collins M. D. 1985; Analysis of isoprenoid quinones. Methods Microbiol 18:329–366 [View Article]
     [Google Scholar]
  7. Collins M. D., Jones D. 1981; Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev 45:316–354[PubMed]
     [Google Scholar]
  8. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
     [Google Scholar]
  9. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
     [Google Scholar]
  10. Garey J., Wolff M. S. 1998; Estrogenic and antiprogestagenic activities of pyrethroid insecticides. Biochem Biophys Res Commun 251:855–859 [View Article][PubMed]
     [Google Scholar]
  11. Guo P., Wang B. Z., Hang B. J., Li L., Ali S. W., He J., Li S. P. 2009; Pyrethroid-degrading Sphingobium sp. JZ-2 and the purification and characterization of a novel pyrethroid hydrolase. Int Biodeterior Biodegradation 63:1107–1112 [View Article]
     [Google Scholar]
  12. Guo P., Wang B. Z., Hang B. J., Li L., Li S. P., He J. 2010; Sphingobium faniae sp. nov., a pyrethroid-degrading bacterium isolated from activated sludge treating wastewater from pyrethroid manufacture. Int J Syst Evol Microbiol 60:408–412 [View Article][PubMed]
     [Google Scholar]
  13. Katsuda Y. 1999; Development of and future prospects for pyrethroid chemistry. Pestic Sci 55:775–782 [View Article]
     [Google Scholar]
  14. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [View Article][PubMed]
     [Google Scholar]
  15. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp. 115–147 Edited by Stackebrandt E., Goodfellow M. New York: Wiley;
     [Google Scholar]
  16. Laskowski D. A. 2002; Physical and chemical properties of pyrethroids. Rev Environ Contam Toxicol 174:49–170[PubMed]
     [Google Scholar]
  17. Maloney S. E., Maule A., Smith A. R. W. 1988; Microbial transformation of the pyrethroid insecticides: permethrin, deltamethrin, Fastac, fenvalerate, and fluvalinate. Appl Environ Microbiol 54:2874–2876[PubMed]
     [Google Scholar]
  18. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B:195–206 [View Article]
     [Google Scholar]
  19. Ohta H., Hattori T. 1983; Agromonas oligotrophica gen. nov., sp. nov., a nitrogen-fixing oligotrophic bacterium. Antonie van Leeuwenhoek 49:429–446[PubMed]
     [Google Scholar]
  20. Prakash O., Lal R. 2006; Description of Sphingobium fuliginis sp. nov., a phenanthrene-degrading bacterium from a fly ash dumping site, and reclassification of Sphingomonas cloacae as Sphingobium cloacae comb. nov.. Int J Syst Evol Microbiol 56:2147–2152 [View Article][PubMed]
     [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
     [Google Scholar]
  22. Sambrook J., Russell D. W. 2001 Molecular cloning: a Laboratory Manual, 3rd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  23. Sasser, M. (1990). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE: MIDI.
  24. Singh A., Lal R. 2009; Sphingobium ummariense sp. nov., a hexachlorocyclohexane (HCH)-degrading bacterium, isolated from HCH-contaminated soil. Int J Syst Evol Microbiol 59:162–166 [View Article][PubMed]
     [Google Scholar]
  25. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp. 607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  26. Smith T. M., Stratton G. W. 1986; Effects of synthetic pyrethroid insecticides on nontarget organisms. Residue Rev 97:93–120[PubMed]
     [Google Scholar]
  27. Suchismita & Anilava 2008; Acute toxicity of synthetic pyrethroid Cypermethrin to some freshwater organisms. Bull Environ Contam Toxicol 80:49–52 [CrossRef]
     [Google Scholar]
  28. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. 2001; Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov.. Int J Syst Evol Microbiol 51:1639–1652[PubMed] [CrossRef]
     [Google Scholar]
  29. Takeuchi M., Hamana K., Hiraishi A. 2001; Proposal of the genus Sphingomonas sensu stricto and three new genera, Sphingobium, Novosphingobium and Sphingopyxis, on the basis of phylogenetic and chemotaxonomic analyses. Int J Syst Evol Microbiol 51:1405–1417[PubMed]
     [Google Scholar]
  30. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetic analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [View Article][PubMed]
     [Google Scholar]
  31. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
     [Google Scholar]
  32. Tindall B. J. 1990a; Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66:199–202 [View Article]
     [Google Scholar]
  33. Tindall B. J. 1990b; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [CrossRef]
     [Google Scholar]
  34. Tindall B. J., Rosselló-Móra R., Busse H.-J., Ludwig W., Kämpfer P. 2010; Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60:249–266 [View Article][PubMed]
     [Google Scholar]
  35. Vaz-Moreira I., Faria C., Lopes A. R., Svensson L., Falsen E., Moore E. R., Ferreira A. C., Nunes O. C., Manaia C. M. 2009; Sphingobium vermicomposti sp. nov., isolated from vermicompost. Int J Syst Evol Microbiol 59:3145–3149 [View Article][PubMed]
     [Google Scholar]
  36. Wang B. Z., Guo P., Hang B. J., Li L., He J., Li S. P. 2009; Cloning of a novel pyrethroid-hydrolyzing carboxylesterase gene from Sphingobium sp. strain JZ-1 and characterization of the gene product. Appl Environ Microbiol 75:5496–5500 [View Article][PubMed]
     [Google Scholar]
  37. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
     [Google Scholar]
  38. Yabuuchi E., Kosako Y., Fujiwara N., Naka T., Matsunaga I., Ogura H., Kobayashi K. 2002; Emendation of the genus Sphingomonas Yabuuchi et al. 1990 and junior objective synonymy of the species of three genera, Sphingobium, Novosphingobium and Sphingopyxis, in conjunction with Blastomonas ursincola . Int J Syst Evol Microbiol 52:1485–1496 [View Article][PubMed]
     [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.023309-0
Loading
Sphingobium wenxiniae sp. nov., a synthetic pyrethroid (SP)-degrading bacterium isolated from activated sludge in an SP-manufacturing wastewater treatment facility
Int J Syst Evol Microbiol 61, 1776 (2011); https://doi.org/10.1099/ijs.0.023309-0
/content/journal/ijsem/10.1099/ijs.0.023309-0
/content/journal/ijsem/10.1099/ijs.0.023309-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error