1887

Abstract

A Gram-stain-positive, rod-shaped, yellow, non-motile, non-spore-forming, strictly aerobic bacterial strain, designated MW 10, was isolated from seawater of the Bay of Bengal, India, and was subjected to a polyphasic taxonomic study. Analysis of the 16S rRNA gene sequence revealed that strain MW 10 showed highest similarity to the type strains of (96.15 %) and (96.01 %) and showed less than 96 % similarity to members of the genera , , and Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain MW 10 formed a clade separate from members of closely related genera. The morphological, physiological and chemotaxonomic characteristics of strain MW 10 differed from those of members of closely related genera. The major fatty acid in strain MW 10 was iso-C and the menaquinones were MK-7 (48.4 %), MK-8 (32.3 %), MK-7(H) (13.7 %) and MK-6 (5.6 %). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unknown phospholipid, an unknown lipid and an unknown glycolipid. The cell-wall peptidoglycan type was -Lys–-Asp. The genomic DNA G+C content (53.4 mol%) of strain MW 10 was significantly different from those of members of closely related genera. On the basis of its morphological, physiological and chemotaxonomic characteristics as well as our phylogenetic analysis, we conclude that strain MW 10 is a member of a novel genus and species, for which the name gen. nov., sp. nov., is proposed. The type strain of is MW 10 ( = MTCC 10098  = JCM 16573).

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2011-08-01
2019-10-14
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Spore staining of cells of strain MW 10 grown on sporulating agar.

IMAGE

Polar lipid profile of strain MW 10 . Plates were sprayed with molybdenum blue to detect phospholipids (a), ninhydrin to detect aminolipids (b) and molybdatophosphoric acid to detect total lipids (c).

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Phylogenetic trees based on the maximum-parsimony (Fig. S3) and minimum-evolution (Fig. S4) methods showing the positions of strain MW 10 and related taxa. [PDF](104 KB)

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