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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 61, Issue 12

sp. nov., a halotolerant root-associated bacterium with plant growth-promoting potential Free

Abstract

A Gram-positive-staining, aerobic, non-motile, coccoid shaped, halotolerant bacterium (strain JG 06) was isolated from the roots of , an extreme halophyte. Phylogenetic analysis based on 16S rRNA gene sequence showed that the novel strain had sequence similarities of 99.2 % to JCM 11608, 99.0 % to DSM 10241 and 98.2 % to DSM 4810. DNA–DNA hybridization with DSM 46341, DSM 10241, DSM 4810, DSM 10673, DSM 10672, DSM 14564, DSM 14566 and DSM 15460 resulted in reassociation values of 36.2 %, 36.5 %, 35.8 %, 27.6 %, 27.9 %, 28.2 %, 28.7 % and 11.2 %, respectively. The peptidoglycan type of strain JG 06 was variant A4γ. The menaquinone content was MK7 (100 %). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, monogalactosyl diglyceride, three unidentified phospholipids and three glycolipids. The predominant fatty acid was anteiso-C (52.07 %); significant amounts of iso-C(12.38 %), iso-C(8.59 %) and anteiso-C(10.03 %) were also present. The G+C content of the DNA was 73.0 mol%. The strain formed a growth pellicle in nitrogen-free semisolid NFb medium containing NaCl at levels of up to 4 % (w/v) and reduced acetylene to ethylene, a result indicative of N fixation. In nutrient broth medium the novel strain grew at NaCl concentrations up to 15 % (w/v). It also had the ability to produce indole-3-acetic acid (IAA) and siderophores, utilized 1-aminocyclopropane-1-carboxylate (ACC) as a sole source of nitrogen and possessed the ACC deaminase enzyme. On the basis of physiological, biochemical data and phylogenetic analyses, strain JG 06 should be placed in the genus . Strain JG 06 represents a novel species of the genus for which the name sp. nov. is proposed (type strain JG 06 = DSM 23186 = IMCC 252).

  • Published Online:
Funding
This study was supported by the:
  • CSIR (Award NWP-18)
  • GSBTM
  • DST
  • Govt. of Gujarat
© 2011 IUMS
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Brachybacterium saurashtrense sp. nov., a halotolerant root-associated bacterium with plant growth-promoting potential
Int J Syst Evol Microbiol 61, 2799 (2011); https://doi.org/10.1099/ijs.0.023176-0
/content/journal/ijsem/10.1099/ijs.0.023176-0
/content/journal/ijsem/10.1099/ijs.0.023176-0
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