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Abstract

A Gram-stain-negative, aerobic, rod-shaped, non-spore-forming bacterium (SV325) was isolated from the sediment of a hypersaline lake located 4600 m above sea level (Laguna Vilama, Argentina). Strain SV325 formed cream to pink colonies, was motile and moderately halophilic, and tolerated NaCl concentrations of 1–25 % (w/v) with an optimum of 5–10 % (w/v). Growth occurred at 5–40 °C (optimum around 30 °C) and at pH 5.0–10.0 (optimum 7.0–8.0). The bacterium did not produce exopolysaccharides and stained positively for intracellular polyphosphate granules but not for poly-β-hydroxyalkanoates. It produced catalase and oxidase, reduced nitrate to nitrite, hydrolysed gelatin, did not produce acids from sugars and utilized a limited range of substrates as carbon and energy sources: acetate, caproate, fumarate, -β-hydroxybutyrate, malate, maleate, malonate and succinate. The predominant ubiquinones were Q-9 (92.5 %) and Q-8 (7.5 %), the major fatty acids were C cyclo ω8, C, C cyclo and Cω7/iso-C 2-OH, and the DNA G+C content was 55.0 mol%. Phylogenetic analyses based on the 16S rRNA gene indicated that strain SV325 belongs to the genus in the class . Physiological and biochemical tests allowed phenotypic differentiation of strain SV325 from closely related species with validly published names. We therefore propose a novel species, sp. nov., with type strain SV325 ( = DSM 21020  = LMG 24332).

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2011-05-01
2020-01-17
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References

  1. Arahal D. R. , Ludwig W. , Schleifer K. H. , Ventosa A. . ( 2002; ). Phylogeny of the family Halomonadaceae based on 23S and 16S rDNA sequence analyses. . Int J Syst Evol Microbiol 52:, 241–249.[PubMed]
    [Google Scholar]
  2. Arahal D. R. , Vreeland R. H. , Litchfield C. D. , Mormile M. R. , Tindall B. J. , Oren A. , Béjar V. , Quesada E. , Ventosa A. . ( 2007; ). Recommended minimal standards for describing new taxa of the family Halomonadaceae . . Int J Syst Evol Microbiol 57:, 2436–2446. [CrossRef] [PubMed]
    [Google Scholar]
  3. Bauer A. W. , Kirby W. M. M. , Sherris J. C. , Turck M. . ( 1996; ). Antibiotic susceptibility testing by a standardized single disk method. . Am J Clin Pathol 45:, 493–496. [CrossRef] [PubMed]
    [Google Scholar]
  4. Ben Ali Gam Z. , Abdelkafi S. , Casalot L. , Tholozan J. L. , Oueslati R. , Labat M. . ( 2007; ). Modicisalibacter tunisiensis gen. nov., sp. nov., an aerobic, moderately halophilic bacterium isolated from an oilfield-water injection sample, and emended description of the family Halomonadaceae Franzmann et al. 1989 emend Dobson and Franzmann 1996 emend. Ntougias et al. 2007. . Int J Syst Evol Microbiol 57:, 2307–2313. [CrossRef] [PubMed]
    [Google Scholar]
  5. Cole J. R. , Chai B. , Farris R. J. , Wang Q. , Kulam-Syed-Mohideen A. S. , McGarrell D. M. , Bandela A. M. , Cardenas E. , Garrity G. M. , Tiedje J. M. . ( 2007; ). The ribosomal database project (RDP-II): introducing myRDP space and quality controlled public data. . Nucleic Acids Res 35: Database issue D169–D172. [CrossRef] [PubMed]
    [Google Scholar]
  6. Cole J. R. , Wang Q. , Cardenas E. , Fish J. , Chai B. , Farris R. J. , Kulam-Syed-Mohideen A. S. , McGarrell D. M. , Marsh T. et al. ( 2009; ). The Ribosomal Database Project: improved alignments and new tools for rRNA analysis. . Nucleic Acids Res 37: Database issue D141–D145. [CrossRef] [PubMed]
    [Google Scholar]
  7. de la Haba R. R. , Arahal D. R. , Márquez M. C. , Ventosa A. . ( 2010; ). Phylogenetic relationships within the family Halomonadaceae based on comparative 23S and 16S rRNA gene sequence analysis. . Int J Syst Evol Microbiol 60:, 737–748. [CrossRef] [PubMed]
    [Google Scholar]
  8. Dobson S. J. , Franzmann P. D. . ( 1996; ). Unification of the genera Deleya (Bauman et al. 1993), Halomonas (Vreeland et al. 1980) and Halovibrio (Fendrich 1988) and the species Paracoccus halodenitrificans (Robinson and Gibbons 1952) into a single genus, Halomonas, and placement of the genus Zymobacter in the family Halomonadaceae . . Int J Syst Bacteriol 46:, 550–558. [CrossRef]
    [Google Scholar]
  9. Dyall-Smith M. . ( 2008; ). The Halohandbook. Protocols for Haloarchaeal Genetics, version 7.0. http://haloarchaea.com/resources/halohandbook/index.html
  10. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  11. Franzmann P. D. , Wehmeyer U. , Stackebrandt E. . ( 1988; ). Halomonadaceae fam. nov., a new family of the class Proteobacteria to accommodate the genera Halomonas and Deleya . . Syst Appl Microbiol 11:, 16–19.[CrossRef]
    [Google Scholar]
  12. Guzmán D. , Quillaguamán J. , Muñoz M. , Hatti-Kaul R. . ( 2010; ). Halomonas andesensis sp. nov., a moderate halophile isolated from the saline lake Laguna Colorada in Bolivia. . Int J Syst Evol Microbiol 60:, 749–753. [CrossRef] [PubMed]
    [Google Scholar]
  13. Horikoshi K. , Grant W. D. . (editors) ( 1998; ). Extremophiles: Microbial Life in Extreme Environments. New York:: Wiley;.
    [Google Scholar]
  14. Hugh R. , Leifson E. . ( 1953; ). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. . J Bacteriol 66:, 24–26.[PubMed]
    [Google Scholar]
  15. Kaye J. Z. , Márquez M. C. , Ventosa A. , Baross J. A. . ( 2004; ). Halomonas neptunia sp. nov., Halomonas sulfidaeris sp. nov., Halomonas axialensis sp. nov. and Halomonas hydrothermalis sp. nov.: halophilic bacteria isolated from deep-sea hydrothermal-vent environments. . Int J Syst Evol Microbiol 54:, 499–511. [CrossRef] [PubMed]
    [Google Scholar]
  16. Kim K. K. , Jin L. , Yang H. C. , Lee S.-T. . ( 2007; ). Halomonas gomseomensis sp. nov., Halomonas janggokensis sp. nov., Halomonas salaria sp. nov. and Halomonas denitrificans sp. nov., moderately halophilic bacteria isolated from saline water. . Int J Syst Evol Microbiol 57:, 675–681. [CrossRef] [PubMed]
    [Google Scholar]
  17. Kumar S. , Tamura K. , Jakobsen I. B. , Nei M. . ( 2001; ). mega2: molecular evolutionary genetics analysis software. . Bioinformatics 17:, 1244–1245. [CrossRef] [PubMed]
    [Google Scholar]
  18. Mata J. A. , Martínez-Cánovas J. , Quesada E. , Béjar V. . ( 2002; ). A detailed phenotypic characterisation of the type strains of Halomonas species. . Syst Appl Microbiol 25:, 360–375. [CrossRef] [PubMed]
    [Google Scholar]
  19. Menes R. J. , Muxí L. . ( 2002; ). Anaerobaculum mobile sp. nov., a novel anaerobic, moderately thermophilic, peptide-fermenting bacterium that uses crotonate as an electron acceptor, and emended description of the genus Anaerobaculum . . Int J Syst Evol Microbiol 52:, 157–164.[PubMed]
    [Google Scholar]
  20. Murray R. G. E. , Doetsch R. N. , Robinow C. F. . ( 1994; ). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, D.C.:: American Society for Microbiology;.
    [Google Scholar]
  21. Rodríguez-Valera F. , Ruiz-Berraquero F. , Ramos-Cormenzana A. . ( 1981; ). Characteristics of the heterotrophic bacterial populations in hypersaline environments of different salt concentrations. . Microb Ecol 7:, 235–243. [CrossRef]
    [Google Scholar]
  22. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  23. Sánchez-Porro C. , Kaur B. , Mann H. , Ventosa A. . ( 2010; ). Halomonas titanicae sp. nov., a halophilic bacterium isolated from the RMS Titanic. . Int J Syst Evol Microbiol 60:, 2768–2774. [CrossRef] [PubMed]
    [Google Scholar]
  24. Seufferheld M. J. , Alvarez H. M. , Farias M. E. . ( 2008; ). Role of polyphosphates in microbial adaptation to extreme environments. . Appl Environ Microbiol 74:, 5867–5874. [CrossRef] [PubMed]
    [Google Scholar]
  25. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, D.C.:: American Society for Microbiology;.
    [Google Scholar]
  26. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  27. Ventosa A. , Nieto J. J. , Oren A. . ( 1998; ). Biology of moderately halophilic aerobic bacteria. . Microbiol Mol Biol Rev 62:, 504–544.[PubMed]
    [Google Scholar]
  28. Vreeland R. H. , Litchfield C. D. , Martin E. L. , Elliot E. . ( 1980; ). Halomonas elongata, a new genus and species of extremely salt-tolerant bacteria. . Int J Syst Bacteriol 30:, 485–495. [CrossRef]
    [Google Scholar]
  29. Xu X.-W. , Wu Y.-H. , Zhou Z. , Wang C.-S. , Zhou Y.-G. , Zhang H.-B. , Wang Y. , Wu M. . ( 2007; ). Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China. . Int J Syst Evol Microbiol 57:, 1619–1624. [CrossRef] [PubMed]
    [Google Scholar]
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Growth rates ( ) at different temperatures and pH for strain SV325 . Bars indicate standard deviations from triplicate runs.

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