1887

Abstract

Three Gram-positive, rod-shaped bacterial strains were isolated from the drinking water supply system of the Hungarian capital, Budapest. Phylogenetic analysis on the basis of 16S rRNA gene sequence comparison revealed that the isolates represented a distinct cluster within the clade of the genus and were most closely related to OS4, GW-9, MSL-01 and DS-30. The peptidoglycan based on -2,6-diaminopimelic acid, the major menaquinone MK-8(H), the cellular fatty acid profile with iso-C and anteiso-C as predominating components and the DNA G+C content of 71.4 mol% (strain 1RaM5-12) were consistent with the affiliation of the isolates to the genus . Because of differences in physiological characteristics, matrix-assisted laser-desorption/ionization time-of-flight mass spectra of protein extracts, II RiboPrinter patterns and 96.1 % 16S rRNA gene sequence similarity between strain 1RaM5-12 and its closest phylogenetic neighbour, OS4, a novel species, sp. nov., is proposed. The type strain is 1RaM5-12 (=DSM 21673 =NCAIM 02330).

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2011-03-01
2020-01-21
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