1887

Abstract

A novel non-sporulating, non-motile, catalase- and oxidase-positive, strictly aerobic, Gram-positive-staining coccus, strain JSM 077023, was isolated from an intertidal sediment sample collected from Naozhou Island in the South China Sea, China. Growth occurred in the presence of 0.5–25 % (w/v) NaCl [optimum, 2–5 % (w/v) NaCl] and at pH 5.5–10.5 (optimum, pH 7.0–8.0) and at 4–45 °C (optimum, 30–35 °C). The major amino acid constituents of the cell wall were alanine, glycine and lysine. The major cellular fatty acids were anteiso-C and iso-C. The strain contained MK-7 and MK-6 as the predominant respiratory quinones and diphosphatidylglycerol, phosphatidylglycerol and an unidentified phospholipid as the polar lipids. The genomic DNA G+C content of strain JSM 077023 was 41.3 mol%. A phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 077023 should be assigned to the genus and was most closely related to the type strains of (sequence similarity 99.0 %) and (99.0 %), followed by (98.6 %) and (97.4 %). 16S rRNA gene sequence similarities of less than 97 % were observed with other species of the genus . Levels of DNA–DNA relatedness between strain JSM 077023 and the type strains of , , and ranged from 36.8 to 22.7 %. The combination of phylogenetic analysis, DNA–DNA relatedness values, phenotypic characteristics and chemotaxonomic data supported the suggestion that strain JSM 077023 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JSM 077023 ( = DSM 23006 = KCTC 13714). An emended description of the genus is also presented.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.022871-0
2011-09-01
2019-10-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/9/2029.html?itemId=/content/journal/ijsem/10.1099/ijs.0.022871-0&mimeType=html&fmt=ahah

References

  1. Atlas R. M. , Parks L. C. . ( 1993; ). Handbook of Microbiological Media. Boca Raton, FL:: CRC Press;.
    [Google Scholar]
  2. Chen Y.-G. , Cui X.-L. , Pukall R. , Li H.-M. , Yang Y.-L. , Xu L.-H. , Wen M.-L. , Peng Q. , Jiang C.-L. . ( 2007; ). Salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, south-west China. . Int J Syst Evol Microbiol 57:, 2327–2332. [CrossRef].[PubMed]
    [Google Scholar]
  3. Chen Y.-G. , Zhang Y.-Q. , Huang K. , Tang S.-K. , Cao Y. , Shi J.-X. , Xiao H.-D. , Cui X.-L. , Li W.-J. . ( 2009a; ). Pigmentiphaga litoralis sp. nov., a facultatively anaerobic bacterium isolated from a tidal flat sediment. . Int J Syst Evol Microbiol 59:, 521–525. [CrossRef].[PubMed]
    [Google Scholar]
  4. Chen Y.-G. , Zhang Y.-Q. , Shi J.-X. , Xiao H.-D. , Tang S.-K. , Liu Z.-X. , Huang K. , Cui X.-L. , Li W.-J. . ( 2009b; ). Jeotgalicoccus marinus sp. nov., a marine bacterium isolated from a sea urchin. . Int J Syst Evol Microbiol 59:, 1625–1629. [CrossRef].[PubMed]
    [Google Scholar]
  5. Chun J. , Lee J.-H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef].[PubMed]
    [Google Scholar]
  6. Collins M. D. , Jones D. . ( 1980; ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. . J Appl Bacteriol 48:, 459–470.[CrossRef]
    [Google Scholar]
  7. Cowan S. T. , Steel K. J. . ( 1965; ). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
    [Google Scholar]
  8. Cui X.-L. , Mao P.-H. , Zeng M. , Li W.-J. , Zhang L.-P. , Xu L.-H. , Jiang C.-L. . ( 2001; ). Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae . . Int J Syst Evol Microbiol 51:, 357–363.[PubMed]
    [Google Scholar]
  9. De Ley J. , Cattoir H. , Reynaerts A. . ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12:, 133–142. [CrossRef].[PubMed]
    [Google Scholar]
  10. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef].[PubMed]
    [Google Scholar]
  11. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  12. Felsenstein J. . ( 2002; ). phylip (phylogeny inference package), version 3.6a. Distributed by the author. . Department of Genome Sciences, University of Washington;, Seattle, USA:.
  13. Gregersen T. . ( 1978; ). Rapid method for distinction of Gram-negative from Gram-positive bacteria. . Eur J Appl Microbiol Biotechnol 5:, 123–127. [CrossRef]
    [Google Scholar]
  14. Guo X.-Q. , Li R. , Zheng L.-Q. , Lin D.-Q. , Sun J.-Q. , Li S.-P. , Li W.-J. , Jiang J.-D. . ( 2010; ). Jeotgalicoccus huakuii sp. nov., a halotolerant bacterium isolated from seaside soil. . Int J Syst Evol Microbiol 60:, 1307–1310. [CrossRef].[PubMed]
    [Google Scholar]
  15. Hopwood D. A. , Bibb M. J. , Chater K. F. , Kieser T. , Bruton C. J. , Kieser H. M. , Lydiate D. J. , Smith C. P. , Ward J. M. . ( 1985; ). Genetic Manipulation of Streptomyces: a Laboratory Manual . Norwich:: John Innes Foundation;.
    [Google Scholar]
  16. Hoyles L. , Collins M. D. , Foster G. , Falsen E. , Schumann P. . ( 2004; ). Jeotgalicoccus pinnipedialis sp. nov., from a southern elephant seal (Mirounga leonina). . Int J Syst Evol Microbiol 54:, 745–748. [CrossRef].[PubMed]
    [Google Scholar]
  17. Huß V. A. R. , Festl H. , Schleifer K. H. . ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4:, 184–192.[CrossRef]
    [Google Scholar]
  18. Jahnke K. D. . ( 1992; ). basic computer program for evaluation of spectroscopic DNA renaturation data from Gilford System 2600 spectrophotometer on a PC/XT/AT type personal computer. . J Microbiol Methods 15:, 61–73. [CrossRef]
    [Google Scholar]
  19. Kimura M. . ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef].[PubMed]
    [Google Scholar]
  20. Kluge A. G. , Farris F. S. . ( 1969; ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18:, 1–32. [CrossRef]
    [Google Scholar]
  21. Kroppenstedt R. M. . ( 1982; ). Separation of bacterial menaquinones by HPLC using reverse phase (RP 18) and a silver loaded ion exchanger. . J Liq Chromatogr 5:, 2359–2367. [CrossRef]
    [Google Scholar]
  22. Kroppenstedt R. M. . ( 1985; ). Fatty acid and menaquinone analysis of actinomycetes and related organisms. . In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20), pp. 173–199. Edited by Goodfellow M. , Minnikin D. E. . . New York:: Academic Press;.
    [Google Scholar]
  23. Martin E. , Klug K. , Frischmann A. , Busse H.-J. , Kämpfer P. , Jäckel U. . ( 2011; ). Jeotgalicoccus coquinae sp. nov. and Jeotgalicoccus aerolatus sp. nov., isolated from poultry houses. . Int J Syst Evol Microbiol 61:, 237–241. [CrossRef].[PubMed]
    [Google Scholar]
  24. Mesbah M. , Premachandran U. , Whitman W. B. . ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39:, 159–167. [CrossRef]
    [Google Scholar]
  25. Minnikin D. E. , Collins M. D. , Goodfellow M. . ( 1979; ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47:, 87–95.[CrossRef]
    [Google Scholar]
  26. Minnikin D. E. , O’Donnell A. G. , Goodfellow M. , Alderson G. , Athalye M. , Schaal A. , Parlett J. H. . ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2:, 233–241. [CrossRef]
    [Google Scholar]
  27. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  28. Sasser M. . ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
  29. Schleifer K. H. . ( 1985; ). Analysis of the chemical composition and primary structure of murein. . Methods Microbiol 18:, 123–156. [CrossRef]
    [Google Scholar]
  30. Smibert R. M. , Krieg N. R. . ( 1994; ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P. , Murray R. G. E. , Wood W. A. , Krieg N. R. . . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  31. Stackebrandt E. , Goebel B. M. . ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  32. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetic analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef].[PubMed]
    [Google Scholar]
  33. Tang S. K. , Wang Y. , Chen Y. , Lou K. , Cao L.-L. , Xu L.-H. , Li W.-J. . ( 2009; ). Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella . . Int J Syst Evol Microbiol 59:, 2025–2032. [CrossRef].[PubMed]
    [Google Scholar]
  34. Thompson J. D. , Gibson T. J. , Plewniak F. , Jeanmougin F. , Higgins D. G. . ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef].[PubMed]
    [Google Scholar]
  35. Ventosa A. , Quesada E. , Rodriguez-Valera F. , Ruiz-Berraquero F. , Ramos-Cormenzana A. . ( 1982; ). Numerical taxonomy of moderately halophilic Gram-negative rods. . J Gen Microbiol 128:, 1959–1968.
    [Google Scholar]
  36. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. et al. ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  37. Yoon J.-H. , Lee K.-C. , Weiss N. , Kang K. H. , Park Y.-H. . ( 2003; ). Jeotgalicoccus halotolerans gen. nov., sp. nov. and Jeotgalicoccus psychrophilus sp. nov., isolated from the traditional Korean fermented seafood jeotgal. . Int J Syst Evol Microbiol 53:, 595–602. [CrossRef].[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.022871-0
Loading
/content/journal/ijsem/10.1099/ijs.0.022871-0
Loading

Data & Media loading...

Supplements

vol. , part 9, pp. 2029 - 2034

Phylogenetic trees showing the phylogenetic position of strain JSM 077023 among phylogenetic neighbours inferred from 16S rRNA gene sequences. The trees were constructed using the maximum-parsimony (a) and maximum-likelihood (b) methods. Bootstrap percentages (>50%) based on 1000 replications are shown. [ PDF] 53 KB



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error