1887

Abstract

Two non-motile, Gram-staining-negative, yellow-pigmented bacterial strains designated HMD1001 and HMD1033 were isolated from the water of a mesotrophic artificial lake in Korea. A phylogenetic tree based on 16S rRNA gene sequences indicated that both strains could be assigned to the genus ; strain HMD1001 appeared most closely related to H7 (96.8 % sequence similarity), DSM 4002 (96.6 %) and DSM 2063 (96.6 %) whereas strain HMD1033 appeared most closely related to LMG 2201 (96.2 %), AT1048 (96.2 %) and AT1042 (96.2 %). The major fatty acids of strain HMD1001 were iso-C (21.5 %), summed feature 3 (comprising Cω6 and/or Cω7; 18.0 %) and iso-C G (7.6 %), whereas those of HMD1033 were summed feature 3 (23.8 %), iso-C 3-OH (16.9 %), iso-C (15.3 %) and anteiso-C (12.1 %). The genomic DNA G+C contents of strains HMD1001 and HMD1033 were 35.9 and 32.2 mol%, respectively. Phylogenetic and phenotypic evidence indicates that strains HMD1001 and HMD1033 represent two novel species of the genus , for which the names sp. nov. (type strain HMD1001  = KCTC 22796  = CECT 7594) and sp. nov. (type strain HMD1033  = KCTC 22825  = CECT 7649) are proposed.

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2012-04-01
2019-10-21
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References

  1. Bergey D. H. , Harrison F. C. , Breed R. S. , Hammer B. W. , Huntoon F. M. . ( 1923; ). Bergey’s Manual of Determinative Bacteriology. Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  2. Bernardet J.-F. , Bowman J. . ( 2006; ). The genus Flavobacterium . . In The Prokaryotes: a Handbook on the Biology of Bacteria, , 3rd edn., vol. 7, pp. 481–531. Edited by Dworkin M. , Falkow S. , Rosenberg E. , Schleifer K. H. , Stackebrandt E. . . New York:: Springer;.
    [Google Scholar]
  3. Bernardet J. F. , Segers P. , Vancanneyt M. , Berthe F. , Kersters K. , Vandamme P. . ( 1996; ). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). . Int J Syst Bacteriol 46:, 128–148. [CrossRef]
    [Google Scholar]
  4. Bernardet J. F. , Nakagawa Y. , Holmes B. . ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef] [PubMed]
    [Google Scholar]
  5. Cho J. C. , Giovannoni S. J. . ( 2003; ). Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the α-Proteobacteria . . Int J Syst Evol Microbiol 53:, 1031–1036. [CrossRef] [PubMed]
    [Google Scholar]
  6. Chun J. , Lee J. H. , Jung Y. , Kim M. , Kim S. , Kim B. K. , Lim Y. W. . ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. . Int J Syst Evol Microbiol 57:, 2259–2261. [CrossRef] [PubMed]
    [Google Scholar]
  7. Cousin S. , Päuker O. , Stackebrandt E. . ( 2007; ). Flavobacterium aquidurense sp. nov. and Flavobacterium hercynium sp. nov., from a hard-water creek. . Int J Syst Evol Microbiol 57:, 243–249. [CrossRef] [PubMed]
    [Google Scholar]
  8. Felsenstein J. . ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef] [PubMed]
    [Google Scholar]
  9. Felsenstein J. . ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  10. Fitch W. M. . ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  11. Kim B.-Y. , Weon H.-Y. , Cousin S. , Yoo S.-H. , Kwon S.-W. , Go S.-J. , Stackebrandt E. . ( 2006; ). Flavobacterium daejeonense sp. nov. and Flavobacterium suncheonense sp. nov., isolated from greenhouse soils in Korea. . Int J Syst Evol Microbiol 56:, 1645–1649. [CrossRef] [PubMed]
    [Google Scholar]
  12. Kim J. H. , Kim K. Y. , Cha C. J. . ( 2009; ). Flavobacterium chungangense sp. nov., isolated from a freshwater lake. . Int J Syst Evol Microbiol 59:, 1754–1758. [CrossRef] [PubMed]
    [Google Scholar]
  13. Lee S. H. , Kim J. M. , Lee J. R. , Park W. , Jeon C. O. . ( 2010; ). Flavobacterium fluvii sp. nov., isolated from stream sediment. . Int J Syst Evol Microbiol 60:, 353–357. [CrossRef]
    [Google Scholar]
  14. MacFaddin J. F. . ( 1980; ). Biochemical Tests for Identification of Medical Bacteria, , 2nd edn.. Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  15. Mesbah M. , Whitman W. B. . ( 1989; ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr 479:, 297–306. [CrossRef] [PubMed]
    [Google Scholar]
  16. Park M. , Ryu S. H. , Vu T. H. , Ro H. S. , Yun P. Y. , Jeon C. O. . ( 2007; ). Flavobacterium defluvii sp. nov., isolated from activated sludge. . Int J Syst Evol Microbiol 57:, 233–237. [CrossRef] [PubMed]
    [Google Scholar]
  17. Saitou N. , Nei M. . ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  18. Tamaki H. , Hanada S. , Kamagata Y. , Nakamura K. , Nomura N. , Nakano K. , Matsumura M. . ( 2003; ). Flavobacterium limicola sp. nov., a psychrophilic, organic-polymer-degrading bacterium isolated from freshwater sediments. . Int J Syst Evol Microbiol 53:, 519–526.[CrossRef]
    [Google Scholar]
  19. Tamura K. , Dudley J. , Nei M. , Kumar S. . ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef] [PubMed]
    [Google Scholar]
  20. Van Trappen S. , Vandecandelaere I. , Mergaert J. , Swings J. . ( 2005; ). Flavobacterium fryxellicola sp. nov. and Flavobacterium psychrolimnae sp. nov., novel psychrophilic bacteria isolated from microbial mats in Antarctic lakes. . Int J Syst Evol Microbiol 55:, 769–772. [CrossRef] [PubMed]
    [Google Scholar]
  21. Wang Z.-W. , Liu Y.-H. , Dai X. , Wang B.-J. , Jiang C.-Y. , Liu S.-J. . ( 2006; ). Flavobacterium saliperosum sp. nov., isolated from freshwater lake sediment. . Int J Syst Evol Microbiol 56:, 439–442. [CrossRef] [PubMed]
    [Google Scholar]
  22. Wayne L. G. , Brenner D. J. , Colwell R. R. , Grimont P. A. D. , Kandler O. , Krichevsky M. I. , Moore L. H. , Moore W. E. C. , Murray R. G. E. . & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  23. Weon H. Y. , Song M. H. , Son J. A. , Kim B. Y. , Kwon S. W. , Go S. J. , Stackebrandt E. . ( 2007; ). Flavobacterium terrae sp. nov. and Flavobacterium cucumis sp. nov., isolated from greenhouse soil. . Int J Syst Evol Microbiol 57:, 1594–1598. [CrossRef] [PubMed]
    [Google Scholar]
  24. Yi H. , Chun J. . ( 2006; ). Flavobacterium weaverense sp. nov. and Flavobacterium segetis sp. nov., novel psychrophiles isolated from the Antarctic. . Int J Syst Evol Microbiol 56:, 1239–1244.[CrossRef]
    [Google Scholar]
  25. Yoon J. H. , Kang S. J. , Lee J. S. , Oh T. K. . ( 2007; ). Flavobacterium terrigena sp. nov., isolated from soil. . Int J Syst Evol Microbiol 57:, 947–950. [CrossRef] [PubMed]
    [Google Scholar]
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