1887

Abstract

A facultatively denitrifying bacterium, strain K601, was isolated at 30 °C from a municipal sewage plant on cyclohexanol as sole carbon source and nitrate as electron acceptor. Under aerobic conditions this strain used acetate, fumarate, lactate, pyruvate, crotonate, indole, glucose, vanillate, 4-hydroxybenzoate, -cresol, -cresol and -cresol. Under denitrifying conditions the strain used cyclohexanol, cyclohexanone, 1,3-cyclohexanedione, 2-cyclohexenone, 1,3-cyclohexanediol ( and ), monocarboxylic acids (C–C), adipate, pimelate, 5-oxocaproate, citrate, 2-oxoglutarate, succinate, malate, crotonate, lactate, pyruvate and fumarate. Cells were short rods, 0·6 μm wide and 1–2 μm long, motile, non-spore-forming, Gram-negative, and catalase- and oxidase-positive. Strain K601 used nitrate, nitrite and oxygen as electron acceptors, but not sulfate, sulfite or fumarate. The DNA G+C content of strain K601 was 66 mol%. Phylogenetic analysis, based on 16S rDNA sequencing, showed that strain K601 represents a separate lineage of the family in the β-subclass of . Based on the high 16S rDNA sequence divergence and phenotypic characteristics, the name gen. nov., sp. nov. is proposed for this strain. The type strain is K601 (=DSM 14773 =CIP 107495).

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2003-01-01
2019-10-16
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