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Abstract

An actinomycete strain, HM7, was isolated from sandy soil from the banks of the Arvand River, Khoramshahr, Iran. In a study conducted using a polyphasic taxonomic approach, it was found that this strain represented a novel species in the genus . Strain HM7 had long, branched substrate mycelia and the aerial mycelium completely fragmented to long chains of rod-shaped spores. The cell wall of strain HM7 contained -diaminopimelic acid, galactose and ribose. The predominant phospholipids were phosphatidylglycerol, phosphatidylethanolamine diphosphatidylglycerol, and phosphatidylcholine. The main menaquinones were MK-10(H), MK-10(H), MK-10(H) and MK-9(H). Strain HM7 showed the highest degree of 16S rRNA gene sequence similarity with UTMC 00102 (99.8 %) and YIM 28A4 (99.3 %), but differed significantly from these type strains based on DNA–DNA hybridization data (37.6 and 38.9 % relatedness, respectively). Physiological, biochemical and phenotypic characteristics allowed strain HM7 to be differentiated from related species. On the basis of results from phenotypic and phylogenetic analyses, strain HM7 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain of this species is HM7 ( = UTMC 00103 = DSM 45278 = CCUG 58831).

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2011-05-01
2020-01-22
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Scanning electron micrograph of strain HM7 after 14 days growth on ISP 2 medium at 28 °C. Bar, 2 µm.

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Two-dimensional TLC of polar lipids of strain HM7 . DPG, diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PC, phosphatidylcholine; PI, phosphatidylinositol.

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