1887

Abstract

A moderately haloalkaliphilic and obligately methylotrophic bacterium (strain M39) with the ribulose monophosphate pathway of carbon assimilation is described. Cells of this methanol and methylamine utilizer are aerobic, Gram-negative, asporogenous, motile short rods, multiplying by binary fission. It is auxotrophic for vitamin B and requires NaHCO or NaCl for growth in alkaline medium. Its cellular fatty acid profile consists primarily of straight-chain saturated C and unsaturated C and C acids. The major ubiquinone is Q-8. The dominant phospholipids are phosphatidylethanolamine and phosphatidylglycerol. Diphosphatidylglycerol is also present. Optimal growth conditions are 25–29 °C, pH 9·0–9·5 and 3–4 % (w/v) NaCl. Cells accumulate the cyclic amino acid ectoine as the main compatible solute. The DNA G+C content is 48·3 mol%. Based on 16S rDNA sequence analysis and DNA–DNA relatedness (25–30 %) with the type strains of marine methylotrophs belonging to the genus , the novel isolate M39 (=VKM B-2251 =ATCC BAA-297) was classified as the type strain of sp. nov.

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2003-01-01
2020-09-28
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