1887

Abstract

Our previous study, based primarily on PCR-denaturing gradient gel electrophoresis and 16S rRNA gene sequencing, focused on the isolation of four bifidobacterial groups from the digestive tract of three bumblebee species. In that study, we proposed that these isolated groups potentially represented novel species of the family . One of the four, , has been described recently. Strains representing two of the other groups have been classified as members of the genus on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA and heat-shock protein 60 () gene sequences. Analysis of 16S rRNA gene sequence similarities revealed that the isolates of the first group were affiliated to YIT 11866, JCM 1302 and ATCC 25911 (96.2, 96.0 and 95.9 % sequence similarity, respectively), together with other bifidobacteria showing lower sequence similarity. Additional representatives of the second group were found to be affiliated to YIT 4097 and ATCC 25911 (96.0 and 96.3 % sequence similarity) and also to other bifidobacteria with lower sequence similarity. These results indicate that the isolates of the two groups belong to novel species within the genus . This observation was further substantiated by the results of partial sequencing of . On the basis of phylogenetic and phenotypic analyses and analysis of 16S rRNA and partial gene sequences, we propose two novel species, sp. nov. (type strain LISLUCIII-P2  = DSM 22766  = CCM 7728) and sp. nov. (type strain JEMLUCVIII-4  = DSM 22767  = CCM 7729).

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2011-06-01
2019-10-23
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[PDF file of Supplementary Figs S1 and S2](435 KB)

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Filamentous parts growing around the solid core of colonies of strains of sp. nov. after 72 h of cultivation on TPY agar under anaerobic conditions. (a) Strain LISLUCIII-P2 ; (b) strain LUCL-P3; (c, d) strain JESLAP-3. Bars, 100 µm.

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