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Abstract

Two slightly halophilic bacterial strains, C1-52 and YD-9, were isolated from Daban and Aiding salt lakes in Xinjiang, China, respectively. The isolates were Gram-positive, non-endospore-forming, non-motile, facultatively anaerobic cocci. Colonies were pale yellow, and a light pink, diffusible pigment was produced after a few additional days of incubation. The isolates grew optimally with 2–3 % (w/v) NaCl, at pH 7.5 and at 30–35 °C. The peptidoglycan type was -Lys–Gly-Ala(Gly). The menaquinones were MK-7 (83.2 %) and MK-6 (16.8 %). The major fatty acids (>10 %) were anteiso-C and iso-C. The DNA G+C content of strains C1-52 and YD-9 was 41.2 and 41.0 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains C1-52 and YD-9 were closely related to YKJ-115 (98.0 and 97.1 % 16S rRNA gene sequence similarity, respectively), followed by YKJ-101 (97.1 and 96.8 %). Strains C1-52 and YD-9 shared, respectively, 20 and 11 % DNA–DNA relatedness with JCM 11198 and 8 and 13 % with JCM 11199. DNA–DNA relatedness between the isolates was 91 %. On the basis of phenotypic and phylogenetic distinctiveness, strains C1-52 and YD-9 belonged to the same species, which should be placed in the genus as a novel species. The name sp. nov. is proposed, with the type strain C1-52 ( = CGMCC 1.8911  = NBRC 105788).

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2011-07-01
2019-10-15
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Transmission electron micrograph of cells of sp. nov. C1-52T. Bar, 1 µm.

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Cellular fatty acid profiles of strains C1-52 and YD-9 and members of the genus . [ PDF] (36 KB)

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