1887

Abstract

A cold-tolerant, yellow-pigmented, Gram-positive, motile, facultatively anaerobic bacterial strain, LI1, was isolated from a moss-covered soil from Livingston Island, Antarctica, near the Bulgarian station St. Kliment Ohridski. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species KFC-22, PON10 and SPC-20, with which it showed sequence similarities of 99.0, 97.9 and 97.9 %, respectively. DNA–DNA hybridization revealed a reassociation value of 2.7 % with LMG 24362. The DNA G+C content of strain LI1 was 64.5 mol%. The growth temperature range was −6 to 28 °C, with optimum growth at 16 °C. Growth occurred in 0–5 % NaCl and at pH 4.5–9.5, with optimum growth in 1–2 % NaCl and at pH 5.5–6.5. The predominant fatty acids were anteiso-C, C and iso-C. The polar lipids were phosphatidylglycerol and diphosphatidylglycerol. Physiological and biochemical tests clearly differentiated strain LI1 from . Therefore, a novel cold-tolerant species within the genus is proposed: sp. nov. (type strain LI1 = DSM 22824 = NCCB 100313).

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2011-08-01
2019-12-09
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