1887

Abstract

A cold-tolerant, yellow-pigmented, Gram-positive, motile, facultatively anaerobic bacterial strain, LI1, was isolated from a moss-covered soil from Livingston Island, Antarctica, near the Bulgarian station St. Kliment Ohridski. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species KFC-22, PON10 and SPC-20, with which it showed sequence similarities of 99.0, 97.9 and 97.9 %, respectively. DNA–DNA hybridization revealed a reassociation value of 2.7 % with LMG 24362. The DNA G+C content of strain LI1 was 64.5 mol%. The growth temperature range was −6 to 28 °C, with optimum growth at 16 °C. Growth occurred in 0–5 % NaCl and at pH 4.5–9.5, with optimum growth in 1–2 % NaCl and at pH 5.5–6.5. The predominant fatty acids were anteiso-C, C and iso-C. The polar lipids were phosphatidylglycerol and diphosphatidylglycerol. Physiological and biochemical tests clearly differentiated strain LI1 from . Therefore, a novel cold-tolerant species within the genus is proposed: sp. nov. (type strain LI1 = DSM 22824 = NCCB 100313).

Funding
This study was supported by the:
  • Deutsche Forschungsgemeinschaft (DFG) (Award WA 1554/4)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.021956-0
2011-08-01
2021-08-03
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/8/1938.html?itemId=/content/journal/ijsem/10.1099/ijs.0.021956-0&mimeType=html&fmt=ahah

References

  1. An S. Y., Xiao T., Yokota A. 2010; Reclassification of Leifsonia aurea to the genus Rhodoglobus as Rhodoglobus aureus comb. nov., and emended description of Rhodoglobus vestalii Sheridan et al. 2003. J Gen Appl Microbiol 56:53–55 [View Article][PubMed]
    [Google Scholar]
  2. Behrendt U., Schumann P., Hamada M., Suzuki K., Spröer C., Ulrich A. 2011; Reclassification of Leifsonia ginsengi (Qiu et al. 2007) as Herbiconiux ginsengi gen. nov., comb. nov. and description of Herbiconiux solani sp. nov., an actinobacterium associated with the phyllosphere of Solanum tuberosum L.. Int J Syst Evol Microbiol 61:1039–1047 [View Article][PubMed]
    [Google Scholar]
  3. Bunt J. S., Rovira A. D. 1955; Microbiological studies of some subantarctic soils. J Soil Sci 6:119–128 [View Article]
    [Google Scholar]
  4. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [View Article][PubMed]
    [Google Scholar]
  5. Dastager S. G., Lee J.-C., Ju Y.-J., Park D.-J., Kim C.-J. 2008; Leifsonia bigeumensis sp. nov., isolated from soil on Bigeum Island, Korea. Int J Syst Evol Microbiol 58:1935–1938 [View Article][PubMed]
    [Google Scholar]
  6. Dastager S. G., Lee J.-C., Ju Y.-J., Park D.-J., Kim C.-J. 2009; Leifsonia kribbensis sp. nov., isolated from soil. Int J Syst Evol Microbiol 59:18–21 [View Article][PubMed]
    [Google Scholar]
  7. Davis M. J., Gillaspie A. G. Jr, Vidaver A. K., Harris R. W. 1984; Clavibacter: a new genus containing some phytopathogenic coryneform bacteria, including Clavibacter xyli subsp. xyli sp. nov., subsp. nov. and Clavibacter xyli subsp. cynodontis subsp. nov., pathogens that cause ratoon stunting disease of sugarcane and bermudagrass stunting disease. Int J Syst Bacteriol 34:107–117 [CrossRef]
    [Google Scholar]
  8. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
    [Google Scholar]
  9. Dojka M. A., Hugenholtz P., Haack S. K., Pace N. R. 1998; Microbial diversity in a hydrocarbon- and chlorinated-solvent-contaminated aquifer undergoing intrinsic bioremediation. Appl Environ Microbiol 64:3869–3877[PubMed]
    [Google Scholar]
  10. DSMZ (2001). Catalogue of Strains, 7th edn. Braunschweig: DSMZ. http://www.dsmz.de/microorganisms/main.php?content_id=35
  11. Evtushenko L. I., Dorofeeva L. V., Subbotin S. A., Cole J. R., Tiedje J. M. 2000; Leifsonia poae gen. nov., sp. nov., isolated from nematode galls on Poa annua, and reclassification of ‘Corynebacterium aquaticum’ Leifson 1962 as Leifsonia aquatica (ex Leifson 1962) gen. nov., nom. rev., comb. nov. and Clavibacter xyli Davis et al. 1984 with two subspecies as Leifsonia xyli (Davis et al. 1984) gen. nov., comb. nov.. Int J Syst Evol Microbiol 50:371–380 [View Article][PubMed]
    [Google Scholar]
  12. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  13. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  14. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  15. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [View Article][PubMed]
    [Google Scholar]
  16. Hu H.-Y., Fujie K., Urano K. 1999; Development of a novel solid phase extraction method for the analysis of bacterial quinones in activated sludge with a higher reliability. J Biosci Bioeng 87:378–382 [View Article][PubMed]
    [Google Scholar]
  17. Hugh R., Leifson E. 1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol 66:24–26[PubMed]
    [Google Scholar]
  18. Huß V. A. R., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [CrossRef]
    [Google Scholar]
  19. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp. 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  20. Kovacs N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178:703 [View Article][PubMed]
    [Google Scholar]
  21. Lee D. W., Lee J. M., Seo J. P., Schumann P., Kim S. J., Lee S. D. 2008; Phycicola gilvus gen. nov., sp. nov., an actinobacterium isolated from living seaweed. Int J Syst Evol Microbiol 58:1318–1323 [View Article][PubMed]
    [Google Scholar]
  22. Leifson E. 1962; The bacterial flora of distilled and stored water. III. New species of the genera Corynebacterium, Flavobacterium, Spirillum and Pseudomonas . Int Bull Bacteriol Nomencl Taxon 12:161–170 [View Article]
    [Google Scholar]
  23. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. et al. 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [View Article][PubMed]
    [Google Scholar]
  24. MacKenzie S. L. 1987; Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70:151–160[PubMed]
    [Google Scholar]
  25. Matsumoto A., Yamada M., Ōmura S., Takahashi Y. 2008; Microterricola viridarii gen. nov., sp. nov., a new member of the family Microbacteriaceae . Int J Syst Evol Microbiol 58:1019–1023 [View Article][PubMed]
    [Google Scholar]
  26. Mesbah M., Premachandran U., Whitman W. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  27. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  28. Pindi P. K., Kishore K. H., Reddy G. S. N., Shivaji S. 2009; Description of Leifsonia kafniensis sp. nov. and Leifsonia antarctica sp. nov.. Int J Syst Evol Microbiol 59:1348–1352 [View Article][PubMed]
    [Google Scholar]
  29. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O. 2007; silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb . Nucleic Acids Res 35:7188–7196 [View Article][PubMed]
    [Google Scholar]
  30. Qiu F., Huang Y., Sun L., Zhang X., Liu Z., Song W. 2007; Leifsonia ginsengi sp. nov., isolated from ginseng root. Int J Syst Evol Microbiol 57:405–408 [View Article][PubMed]
    [Google Scholar]
  31. Ravenschlag K., Sahm K., Pernthaler J., Amann R. 1999; High bacterial diversity in permanently cold marine sediments. Appl Environ Microbiol 65:3982–3989[PubMed]
    [Google Scholar]
  32. Reddy G. S. N., Prakash J. S. S., Srinivas R., Matsumoto G. I., Shivaji S. 2003; Leifsonia rubra sp. nov. and Leifsonia aurea sp. nov., psychrophiles from a pond in Antarctica. Int J Syst Evol Microbiol 53:977–984 [View Article][PubMed]
    [Google Scholar]
  33. Reddy G. S. N., Prabagaran S. R., Shivaji S. 2008; Leifsonia pindariensis sp. nov., isolated from the Pindari glacier of the Indian Himalayas, and emended description of the genus Leifsonia . Int J Syst Evol Microbiol 58:2229–2234 [View Article][PubMed]
    [Google Scholar]
  34. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  35. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477[PubMed]
    [Google Scholar]
  36. Schröder H. 1991 Mikrobiologisches Praktikum, 5th edn. Berlin: Volk und Wissen Verlag;
    [Google Scholar]
  37. Süssmuth R., Eberspächer J., Haag R., Springer W. 1999 Mikrobiologisch-Biochemisches Praktikum, 2nd edn. Stuttgart: Georg Thieme Verlag;
    [Google Scholar]
  38. Suzuki K. I., Suzuki M., Sasaki J., Park Y. H., Komagata K. K. 1999; Leifsonia gen. nov., a genus for 2,4-diaminobutyric acid-containing actinomycetes to accommodate “Corynebacterium aquaticum” Leifson 1962 and Clavibacter xyli subsp. cynodontis Davis et al. 1984. J Gen Appl Microbiol 45:253–262 [View Article][PubMed]
    [Google Scholar]
  39. Zink K.-G., Mangelsdorf K. 2004; Efficient and rapid method for extraction of intact phospholipids from sediments combined with molecular structure elucidation using LC-ESI-MS-MS analysis. Anal Bioanal Chem 380:798–812 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.021956-0
Loading
/content/journal/ijsem/10.1099/ijs.0.021956-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error