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Abstract

Here, we describe a novel bacterium, strain TUD-YJ37, which can accumulate polyhydroxybutyrate (PHB) to more than 85 % (w/w) dry cell weight. The bacterium was isolated from a mixed-culture bioreactor by using a feast–famine regime and its properties were characterized. Phylogenetic analysis based on full 16S rRNA gene sequences revealed that the isolate is a member of the , forming an independent, deep phylogenetic lineage. It is most closely related to members of the genera , and , with sequence similarities below 91 %. Strain TUD-YJ37 was an obligately aerobic, ovoid, Gram-negative bacterium, motile by means of a polar flagellum. It utilized C–C fatty acids as carbon and energy sources. The temperature range for growth was 20–35 °C, with an optimum of 30 °C; the pH range was 6.0–8.0, without a clear optimum. The major respiratory quinone was Q-8. Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified phospholipids, an unidentified aminolipid and another unidentified lipid. The predominant fatty acids in the membrane polar lipids were Cω7, C and Cω7. The G+C content of the genomic DNA was 67.4 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, the isolate is proposed to represent a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is TUD-YJ37 ( = DSM 23606  = CBS 122990).

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2011-09-01
2019-10-22
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vol. , part 9, pp. 2314 - 2319

Polar lipid profile of strain TUD-YJ37 . DPG, Diphosphatidylglycerol; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PL1–PL3, unidentified phospholipids; AL, unidentified aminolipid; L1, unidentified lipid.



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