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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 61, Issue 9

gen. nov., sp. nov., a polyhydroxyalkanoate-accumulating gammaproteobacterium from a sequencing-batch bioreactor Free

Abstract

Here, we describe a novel bacterium, strain TUD-YJ37, which can accumulate polyhydroxybutyrate (PHB) to more than 85 % (w/w) dry cell weight. The bacterium was isolated from a mixed-culture bioreactor by using a feast–famine regime and its properties were characterized. Phylogenetic analysis based on full 16S rRNA gene sequences revealed that the isolate is a member of the , forming an independent, deep phylogenetic lineage. It is most closely related to members of the genera , and , with sequence similarities below 91 %. Strain TUD-YJ37 was an obligately aerobic, ovoid, Gram-negative bacterium, motile by means of a polar flagellum. It utilized C–C fatty acids as carbon and energy sources. The temperature range for growth was 20–35 °C, with an optimum of 30 °C; the pH range was 6.0–8.0, without a clear optimum. The major respiratory quinone was Q-8. Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified phospholipids, an unidentified aminolipid and another unidentified lipid. The predominant fatty acids in the membrane polar lipids were Cω7, C and Cω7. The G+C content of the genomic DNA was 67.4 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, the isolate is proposed to represent a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is TUD-YJ37 ( = DSM 23606  = CBS 122990).

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Funding
This study was supported by the:
  • Netherlands Organization for Scientific Research (NWO)
  • Foundation for Technical Sciences (STW)
© 2011 IUMS
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2011-09-01
2025-04-30
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Plasticicumulans acidivorans gen. nov., sp. nov., a polyhydroxyalkanoate-accumulating gammaproteobacterium from a sequencing-batch bioreactor
Int J Syst Evol Microbiol 61, 2314 (2011); https://doi.org/10.1099/ijs.0.021410-0
/content/journal/ijsem/10.1099/ijs.0.021410-0
/content/journal/ijsem/10.1099/ijs.0.021410-0
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