1887

Abstract

An aerobic, brown-pigmented, non-spore-forming, endophytic bacterium, designated strain Zy-3, was isolated from root nodules of , a native leguminous herb belonging to the family Leguminosae growing in north-western China. Cells of strain Zy-3 were non-motile, Gram-negative rods. Strain Zy-3 produced siderophores and showed antifungal activity. Phylogenetic analysis of the 16S rRNA gene sequence indicated that the closest relative of this organism was HN-182 (96.6 % sequence similarity). On the basis of genotype, fatty acid patterns and physiological characteristics, a novel species sp. nov. is proposed, with Zy-3 (=ACCC 05413 =HAMBI 3106) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.021071-0
2011-03-01
2021-03-01
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/3/665.html?itemId=/content/journal/ijsem/10.1099/ijs.0.021071-0&mimeType=html&fmt=ahah

References

  1. Allen M. B. 1959; Studies with Cyanidium caldarium , an anomalously pigmented chlorophyte. Arch Mikrobiol 32:270–277 [CrossRef]
    [Google Scholar]
  2. Chung Y. C., Kobayashi T., Kanai H., Akiba T., Kudo T. 1995; Purification and properties of extracellular amylase from the hyperthermophilic archaeon Thermococcus profundus DT5432. Appl Environ Microbiol 61:1502–1506
    [Google Scholar]
  3. Collins M. D. 1985; Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp 267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  4. Cowan S. T., Steel K. J. 1965 Manual for the Identification of Medical Bacteria London: Cambridge University Press;
    [Google Scholar]
  5. Davis D. H., Doudoroff M., Stanier R. Y., Mandel M. 1969; Proposal to reject the genus Hydrogenomonas : taxonomic implications. Int J Syst Bacteriol 19:375–390 [CrossRef]
    [Google Scholar]
  6. Dong X.-Z., Cai M. -Y. 2001; Determinative Manual for Routine Bacteriology. Beijing: : Scientific Press (English translation)
  7. Felsenstein J. 1989; phylip – phylogeny inference package (version 3.2). Cladistics 5:164–166
    [Google Scholar]
  8. Fraser P. D., Miura Y., Misawa N. 1997; In vitro characterisation of astaxanthin biosynthetic enzymes. J Biol Chem 272:6128–6135 [CrossRef]
    [Google Scholar]
  9. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors) 1994 Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;
    [Google Scholar]
  10. Hugh R., Leifson E. 1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol 66:24–26
    [Google Scholar]
  11. Katayama Y., Hiraishi A., Kuraishi H. 1995; Paracoccus thiocyanatus sp. nov., a new species of thiocyanate-utilizing facultative chemolithotroph, and transfer of Thiobacillus versutus to the genus Paracoccus as Paracoccus versutus comb. nov. with emendation of the genus. Microbiology 141:1469–1477 [CrossRef]
    [Google Scholar]
  12. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67
    [Google Scholar]
  13. Lipski A., Altendorf K. 1997; Identification of heterotrophic bacteria isolated from ammonia-supplied experimental biofilters. Syst Appl Microbiol 20:448–457 [CrossRef]
    [Google Scholar]
  14. Liu Z.-P., Wang B.-J., Liu X.-Y., Dai X., Liu Y.-H., Liu S.-J. 2008; Paracoccus halophilus sp. nov., isolated from marine sediment of the South China Sea, China, and emended description of genus Paracoccus Davis 1969. Int J Syst Evol Microbiol 58:257–261 [CrossRef]
    [Google Scholar]
  15. Loper J. E., Buyer J. S. 1991; Siderophores in microbial interactions on plant surfaces. Mol Plant Microbe Interact 4:5–13 [CrossRef]
    [Google Scholar]
  16. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  17. Mila I., Scalbert A., Expert D. 1996; Iron withholding by plant polyphenols and resistance to pathogens and rots. Phytochemistry 42:1551–1555 [CrossRef]
    [Google Scholar]
  18. Owen R. J., Hill L. R., Lapage S. P. 1969; Determination of DNA base compositions from melting profiles in dilute buffers. Biopolymers 7:503–516 [CrossRef]
    [Google Scholar]
  19. Richardson N., Davies J. A., Radüchel B. 1999; Iron (III)-based contrast agents for magnetic resonance imaging. Polyhedron 18:2457–2482 [CrossRef]
    [Google Scholar]
  20. Robinson R. W., Akin D. E., Nordstedt R. A., Thomas M. V., Aldrich H. C. 1984; Light and electron microscopic examinations of methane-producing biofilms from anaerobic fixed-bed reactors. Appl Environ Microbiol 48:127–136
    [Google Scholar]
  21. Sasser M. 1990; Identification of bacteria through fatty acid analysis. In Methods in Phytobacteriology pp 199–204 Edited by Klement Z., Rudolph K., Sands D. C. Budapest: Akademiai Kiado;
    [Google Scholar]
  22. Schwyn B., Neilands J. B. 1987; Universal chemical assay for the detection and determination of siderophores. Anal Biochem 160:47–56 [CrossRef]
    [Google Scholar]
  23. Skerman V. B. D. 1967 A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins;
    [Google Scholar]
  24. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  25. Terefework Z., Kaijalainen S., Lindström K. 2001; AFLP fingerprinting as a tool to study the genetic diversity of Rhizobium galegae isolated from Galega orientalis and Galega officinalis . J Biotechnol 91:169–180 [CrossRef]
    [Google Scholar]
  26. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  27. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  28. Van Berkum P., Beyene D., Eardly B. D. 1996; Phylogenetic relationships among Rhizobium species nodulating the common bean ( Phaseolus vulgaris L.). Int J Syst Bacteriol 46:240–244 [CrossRef]
    [Google Scholar]
  29. Van de Peer Y., De Wachter R. 1994; treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10:569–570
    [Google Scholar]
  30. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
    [Google Scholar]
  31. Wu C., Lu X., Qin M., Wang Y., Ruan J. 1989; Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16176–178
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.021071-0
Loading
/content/journal/ijsem/10.1099/ijs.0.021071-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error