1887

Abstract

A bacterial strain, PY2, capable of oxidizing carbon monoxide, was isolated from a soil sample collected from a roadside at Yonsei University, Seoul, Korea. On the basis of 16S rRNA gene sequence analysis, strain PY2 was shown to belong to the genus and was most closely related to LR-26 (99.1 % similarity). Strain PY2 was characterized chemotaxonomically as having iso-C as the predominant fatty acid, MK-8(H) as the major menaquinone, -diaminopimelic acid as the diagnostic diamino acid of the cell wall, as possessing a polar lipid profile that included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and unknown amino-containing phosphoglycolipids, and having a DNA GC content of 75.6 mol%. DNA–DNA relatedness values between strain PY2 and the type strains of , , and were 20.0 %, 22.9 %, 35.9 % and 64.5 %, respectively. Based on the combined evidence from the phylogenetic analyses, chemotaxonomic data and DNA–DNA hybridization experiments, it is proposed that strain PY2 represents a novel species for which the name sp. nov. is proposed. The type strain is PY2 (=KCCM 42922=JCM 16259).

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2011-03-01
2019-10-14
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References

  1. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. ( 1995; ). Current Protocols in Molecular Biology. Boston, MA. : Wiley.
    [Google Scholar]
  2. Collins, M. D., Dorsch, M. & Stackebrandt, E. ( 1989; ). Transfer of Pimelobacter tumescens to Terrabacter gen. nov. as Terrabacter tumescens comb. nov. and of Pimelobacter jensenii to Nocardioides as Nocardioides jensenii comb. nov. Int J Syst Bacteriol 39, 1–6.[CrossRef]
    [Google Scholar]
  3. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  4. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  5. Jensen, H. L. ( 1934; ). Corynebacteria as an important group of soil microorganisms. Proc Linn Soc N S W 59, 19–61.
    [Google Scholar]
  6. Kim, Y. M. & Hegeman, G. D. ( 1981; ). Purification and some properties of carbon monoxide dehydrogenase from Pseudomonas carboxydohydrogena. J Bacteriol 148, 904–911.
    [Google Scholar]
  7. Kim, Y. M. & Hegeman, G. D. ( 1983; ). Oxidation of carbon monoxide by bacteria. Int Rev Cytol 81, 1–32.
    [Google Scholar]
  8. Kraut, M., Hugendieck, I., Herwig, S. & Meyer, O. ( 1989; ). Homology and distribution of CO dehydrogenase structural genes in carboxydotrophic bacteria. Arch Microbiol 152, 335–341.[CrossRef]
    [Google Scholar]
  9. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  10. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt, E. & Goodfellow, M.. London. : Wiley.
    [Google Scholar]
  11. Lee, J. E., Seo, J. P., Lee, D. W., Ko, Y. H. & Lee, S. D. ( 2008; ). Terrabacter lapilli sp. nov., an actinomycete isolated from stone. Int J Syst Evol Microbiol 58, 1084–1088.[CrossRef]
    [Google Scholar]
  12. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  13. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  14. Montero-Barrientos, M., Rivas, R., Velazquez, E., Monte, E. & Roig, M. G. ( 2005; ). Terrabacter terrae sp. nov., a novel actinomycete isolated from soil in Spain. Int J Syst Evol Microbiol 55, 2491–2495.[CrossRef]
    [Google Scholar]
  15. Nicholas, R. A. J., Khan, L. A., Houshaymi, B., Miles, R. J., Ayling, R. D., Hotzel, H. & Sachse, K. ( 2002; ). Close genetic and phenotypic relatedness between mycoplasma ovine/caprine serogroup 11 and Mycoplasma bovigenitalium. Syst Appl Microbiol 25, 396–402.[CrossRef]
    [Google Scholar]
  16. Park, S. W., Park, S. T., Lee, J. E. & Kim, Y. M. ( 2008; ). Pseudonocardia carboxydivorans sp. nov., a carbon monoxide-oxidizing actinomycete, and an emended description of the genus Pseudonocardia. Int J Syst Evol Microbiol 58, 2475–2478.[CrossRef]
    [Google Scholar]
  17. Park, S. W., Kim, S. M., Park, S. T. & Kim, Y. M. ( 2009; ). Tsukamurella carboxydivorans sp. nov., a carbon monoxide oxidizing actinomycete. Int J Syst Evol Microbiol 59, 1541–1544.[CrossRef]
    [Google Scholar]
  18. Reasoner, D. J. & Geldreich, E. E. ( 1979; ). A new medium for the enumeration and subculture of bacteria from potable water. In Abstracts of the 79th Annual Meeting of the American Society for Microbiology, abstract no. N7. Washington, DC: American Society for Microbiology.
  19. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  20. Sasser, M. ( 1990; ). Identification of bacteria through fatty acid analysis. In Methods in Phytobacteriology, pp. 199–204. Edited by Klement, Z., Rudolph, K. & Sands, D. C.. Budapest. : Akademiai Kiado.
    [Google Scholar]
  21. Smibert, R. M. & Krieg, N. R. ( 1981; ). General characterization. In Manual of methods for general bacteriology, pp. 409–443. Edited by Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R.. Washington, DC. : American Society for Microbiology.
    [Google Scholar]
  22. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  23. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  24. Weon, H. Y., Schumann, P., Kroppenstedt, R. M., Kim, B. Y., Song, J., Kwon, S. W., Go, S. J. & Stackebrandt, E. ( 2007; ). Terrabacter aerolatus sp. nov., isolated from an air sample. Int J Syst Evol Microbiol 57, 2106–2109.[CrossRef]
    [Google Scholar]
  25. . Wu, C., Lu, X., Qin, M., Wang, Y. & Ruan, J. ( 1989; ). ). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16, 176–178.
    [Google Scholar]
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vol. , part 3, pp. 482 - 486

Comparison of the fatty acid composition (%) of strain PY2 with other closely related species of the genus . Two-dimensional thin-layer chromatograms of polar lipids from strain PY2 . Lipid spots stained with molybdenum blue after TLC of preparation. Combined [ PDF] file



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