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Abstract

During surveys on yeast diversity in forest soils from Taiwan and Thailand, ten yeast strains isolated from different samples were found to have similar molecular and physiological characteristics. Sequence analysis of small subunit (SSU) rDNA, the D1/D2 domain of large subunit (LSU) rDNA and internal transcribed spacer (ITS)-5.8S rDNA demonstrated that these strains were closely related to . The novel strains could be differentiated from by a 0.9 % sequence divergence (5 substitutions, 0 gaps) in the D1/D2 domain of LSU rDNA, a 1.5 % divergence (8 substitutions, 0 gaps) in the ITS-5.8S rDNA and a 0.7 % divergence (12 substitutions, 2 gaps) in the SSU rDNA. The novel strains also showed specific patterns of electrophoretic karyotypes that differed from that of . Therefore, a novel yeast species, sp. nov., is proposed to accommodate these strains. The type strain SJ7S11 (=BCRC 23194=CBS 11433) was assigned and deposited in the Bioresource Collection and Research Center (BCRC), Food Industry Development and Research Institute, Hsinchu, Taiwan, and Centraalbureau voor Schimmelcultures (CBS), Utrecht, The Netherlands.

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2011-03-01
2020-01-27
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References

  1. Ahearn, D. G., Yarrow, D. & Meyers, S. P. ( 1970; ). Pichia spartinae sp. n. from Louisiana marshland habitats. Antonie van Leeuwenhoek 36, 503–508.[CrossRef]
    [Google Scholar]
  2. Billon-Grand, G. ( 1989; ). A new ascosporogenous yeast genus: Yamadazyma gen. nov. Mycotaxon 35, 201–204.
    [Google Scholar]
  3. Carle, G. F. & Olson, M. V. ( 1985; ). An electrophoretic karyotype for yeast. Proc Natl Acad Sci U S A 82, 3756–3760.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  5. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  6. Kurtzman, C. P. & Robnett, C. J. ( 1998; ). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73, 331–371.[CrossRef]
    [Google Scholar]
  7. Kurtzman, C. P. & Suzuki, M. ( 2010; ). Phylogenetic analysis of ascomycete yeasts that form coenzyme Q-9 and the proposal of the new genera Babjeviella, Meyerozyma, Millerozyma, Priceomyces, and Scheffersomyces. Mycoscience 51, 2–14.[CrossRef]
    [Google Scholar]
  8. Lee, C. F., Liu, C. H., Young, S. S. & Chang, K. S. ( 2008; ). Kazachstania jiainicus sp. nov., an ascomycetous yeast species isolated from soil in Taiwan. FEM Yeast Res 8, 114–118.[CrossRef]
    [Google Scholar]
  9. Lee, C. F., Yao, C. H., Liu, Y. R., Hsieh, C. W. & Young, S. S. ( 2009a; ). Lachancea dasiensis sp. nov., an ascosporogenous yeast isolated from soil and leaves in Taiwan. Int J Syst Evol Microbiol 59, 1818–1822.[CrossRef]
    [Google Scholar]
  10. Lee, C. F., Yao, C. H., Liu, Y. R., Young, S. S. & Chang, K. S. ( 2009b; ). Kazachstania wufongensis sp. nov., an ascosporogenous yeast isolated from soil in Taiwan. Antonie van Leeuwenhoek 95, 335–341.[CrossRef]
    [Google Scholar]
  11. Limtong, S., Yongmanitchai, W., Kawasaki, H. & Fujiyama, K. ( 2009; ). Wickerhamomyces edaphicus sp. nov. and Pichia jaroonii sp. nov., two ascomycetous yeast species isolated from forest soil in Thailand. FEM Yeast Res 9, 504–510.[CrossRef]
    [Google Scholar]
  12. Querol, A., Barrio, E. & Ramon, D. ( 1992; ). A comparative study of different methods of yeast strain characterization. Syst Appl Microbiol 15, 439–446.[CrossRef]
    [Google Scholar]
  13. Stoltenburg, R., Klinner, U., Ritzerfeld, P., Zimmermann, M. & Emeis, C. C. ( 1992; ). Genetic diversity of the yeast Candida utilis. Curr Genet 22, 441–446.[CrossRef]
    [Google Scholar]
  14. Suzuki, M. & Nakase, T. ( 2002; ). A phylogenetic study of ubiquinone-7 species of the genus Candida based on 18S ribosomal DNA sequence divergence. J Gen Appl Microbiol 48, 55–65.[CrossRef]
    [Google Scholar]
  15. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  16. White, T. J., Bruns, T., Lee, S. & Taylor, J. ( 1990; ). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: a Guide for Methods and Applications, pp. 315–322. Edited by Innis, M. A., Gelfand, D. H., Sninsky, J. J. & White, T. J.. New York. : Academic Press.
    [Google Scholar]
  17. Yarrow, D. ( 1998; ). Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn, pp. 77–100. Edited by Kurtzman, C. P. & Fell, J. W.. Amsterdam. : Elsevier.
    [Google Scholar]
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vol. , part 3, pp. 690 - 694

GenBank accession numbers for the LSU, SSU and ITS-5.8S gene sequences determined in this study.



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