1887

Abstract

A novel bacterial strain, designated M2T2B15, was isolated from fermented bovine products and was characterized by using a polyphasic approach. Colonies were reddish pink and circular with entire margins. Cells were strictly aerobic, Gram-reaction-negative, oxidase- and catalase-positive rods that lacked flagella and were motile by gliding. Flexirubin-type pigments were absent. 16S rRNA gene sequence analysis indicated that strain M2T2B15 was related most closely to LMG 22510 (94.4 % similarity) but shared <87 % similarity with other members of the phylum . The major cellular fatty acids were C 5, iso-C and iso-C 3-OH. The polar lipids were phosphatidylethanolamine, phosphatidylserine, two unidentified aminophospholipids and two unidentified polar lipids. Menaquinone 7 (MK-7) was the major respiratory quinone. The G+C content of the DNA of strain M2T2B15 was 52 mol%. The phenotypic, genotypic and phylogenetic data presented clearly indicate that strain M2T2B15 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is M2T2B15 (=KACC 14040 =NBRC 106324). Emended descriptions of the genus and of Vancanneyt 2006 are also proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.020453-0
2011-01-01
2020-01-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/1/30.html?itemId=/content/journal/ijsem/10.1099/ijs.0.020453-0&mimeType=html&fmt=ahah

References

  1. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. (editors) ( 1987; ). Current Protocols in Molecular Biology. New York. : Greene/Wiley Interscience.
    [Google Scholar]
  2. Belina, E., David, P. M. M. & Pillai, M. A. K. ( 2005; ). Effect of Cow's-five (Panchakavya) on Spodoptera litura and Liriomyza trifolii. Madras Agric J 92, 595–598.
    [Google Scholar]
  3. Bernardet, J.-F., Nakagawa, Y. & Holmes, B. ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef]
    [Google Scholar]
  4. Breznak, J. A. & Costilow, R. N. ( 1994; ). Physiological factors in growth. In Methods for General and Molecular Bacteriology, pp. 137–154. Edited by Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R.. Washington, DC. : American Society for Microbiology.
    [Google Scholar]
  5. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y.-W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  8. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  9. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  10. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  11. Kwon, S. W., Kim, J. S., Park, I. C., Yoon, S. H., Park, D. H., Lim, C. K. & Go, S. J. ( 2003; ). Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. Int J Syst Evol Microbiol 53, 21–27.[CrossRef]
    [Google Scholar]
  12. Ludwig, W., Euzéby, J. & Whitman, W. B. ( 2008; ). Draft taxonomic outline of the Bacteroidetes, Planctomycetes, Chlamydiae, Spirochaetes, Fibrobacteres, Fusobacteria, Acidobacteria, Verrucomicrobia, Dictyoglomi, and Gemmatimonadetes for volume 4 of the second edition of Bergey's Manual of Systematic Bacteriology (http://www.bergeys.org/outlines.html. ).
  13. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  14. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  15. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  16. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R.. Washington, DC. : American Society for Microbiology.
    [Google Scholar]
  17. Somasundaram, E., Amanullah, M. M., Thirukkumaran, K., Chandrasekaran, R., Vaiyapuri, K. & Sathyamoorthi, K. ( 2007; ). Biochemical changes, nitrogen flux and yield of crops due to organic sources of nutrients under maize based cropping system. J Appl Sci Res 3, 1724–1729.
    [Google Scholar]
  18. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  19. Vancanneyt, M., Nedashkovskaya, O. I., Snauwaert, C., Mortier, S., Vandemeulebroecke, K., Hoste, B., Dawyndt, P., Frolova, G. M., Janssens, D. & Swings, J. ( 2006; ). Larkinella insperata gen. nov., sp. nov., a bacterium of the phylum ‘Bacteroidetes’ isolated from water of a steam generator. Int J Syst Evol Microbiol 56, 237–241.[CrossRef]
    [Google Scholar]
  20. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J. ( 1991; ). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.020453-0
Loading
/content/journal/ijsem/10.1099/ijs.0.020453-0
Loading

Data & Media loading...

Supplements

vol. , part 1, pp. 30 - 34

Polar lipid profiles of strain M2T2B15 (a) and LMG 22510 (b) after separation by two-dimensional TLC and spraying with phosphomolybdic acid, ninhydrin, α-naphtholsulfuric acid and Dragendorff reagent. PE, phosphatidylethanolamine; PS, phosphatidylserine; L1 & L2, unidentified polar lipids; APL1 & APL2, unidentified aminophospholipids.



IMAGE

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error