1887

Abstract

Twenty-nine rhizobial isolates from root nodules of and spp. growing in the Loess Plateau of China were characterized by numerical taxonomy, RFLP and sequencing of PCR-amplified 16S rRNA genes, measurement of DNA G+C content, DNA–DNA relatedness and cross-nodulation with selected legume species. Based on the results of numerical taxonomy, the isolates formed two clusters (1 and 2) with some single isolates at a similarity level of 82 %. Cluster 1 contained six isolates from and spp. Cluster 2 consisted of nine isolates from spp. DNA relatedness was greater than 80 % among isolates within cluster 2. Phylogenetic analysis based on 16S rRNA gene sequences showed that CCBAU 7190B, representing cluster 2, was closely related to and . DNA–DNA relatedness between CCBAU 7190B and reference strains of , and other related species ranged from 0 to 48·6 %. The cluster 2 isolates could also be differentiated phenotypically from related species. Based on these data, a novel species, sp. nov., is proposed for cluster 2, with the type strain CCBAU 7190B (=AS1.3401=LMG 21975).

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2003-09-01
2019-10-22
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Reference strains used in this research. [PDF](133 KB)

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Extended phylogenetic tree based on 16S rDNA sequences showing the position of the novel species. Nucleotide sequences of 16S rRNA genes obtained in this research and from the database were aligned, sequence similarities were calculated and a phylogenetic tree generated, bootstrapped with 1000 subsamples and visualized as described in Methods. [PDF](118 KB)

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