1887

Abstract

A novel Gram-negative, rod-shaped, non-motile bacterium, designated strain LW7, was isolated from a water sample collected at a depth of 4.5 m from Lonar Lake in Buldhana district, Maharastra, India. The cell suspension was dark-reddish orange due to the presence of carotenoids. The fatty acids were dominated by large amounts of iso-C (59.6 %) and iso-C 3-OH (8.9 %). Strain LW7 contained MK-4 and MK-5 as the major respiratory quinones and phosphatidylglycerol and phosphatidylethanolamine as the major phospholipids. 16S rRNA gene sequence analysis indicated that , a member of family ‘’ (phylum ), is the closest related species, with a sequence similarity of 94.0 % to the type strain. Other members of the family ‘’ had sequence similarities of <93.3 %. Based on the above-mentioned phenotypic and phylogenetic characteristics, strain LW7 is proposed as a representative of a new genus and species, gen. nov., sp. nov. The type strain of is LW7 (=CCUG 57665 =JCM 15946 =NCCB 100279). The genomic DNA G+C of strain LW7 is 49 mol%.

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2010-10-01
2020-01-27
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References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef]
    [Google Scholar]
  2. Anil Kumar, P., Srinivas, T. N. R., Madhu, S., Ruth Manorama, R. & Shivaji, S. ( 2010; ). Indibacter alkaliphilus gen. nov., sp. nov., an alkaliphilic bacterium isolated from a haloalkaline lake. Int J Syst Evol Microbiol 60, 721–726.[CrossRef]
    [Google Scholar]
  3. Arun, A. B., Young, C. C., Chen, W. M., Hung, M. H., Lai, W. A., Chou, J. H., Rekha, P. D., Shen, F. T. & Su, S. P. ( 2009; ). Belliella pelovolcani sp. nov., isolated from a mud-volcano in Taiwan. Int J Syst Evol Microbiol 59, 2534–2537.[CrossRef]
    [Google Scholar]
  4. Bowman, J. P., Nichols, C. M. & Gibson, J. A. ( 2003; ). Algoriphagus ratkowskyi gen. nov., sp. nov., Brumimicrobium glaciale gen. nov., sp. nov., Cryomorpha ignava gen. nov., sp. nov. and Crocinitomix catalasitica gen. nov., sp. nov., novel flavobacteria isolated from various polar habitats. Int J Syst Evol Microbiol 53, 1343–1355.[CrossRef]
    [Google Scholar]
  5. Brettar, I., Christen, R. & Höfle, M. G. ( 2004a; ). Belliella baltica gen. nov., sp. nov., a novel marine bacterium of the CytophagaFlavobacteriumBacteroides group isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 54, 65–70.[CrossRef]
    [Google Scholar]
  6. Brettar, I., Christen, R. & Höfle, M. G. ( 2004b; ). Aquiflexum balticum gen. nov., sp. nov., a novel marine bacterium of the CytophagaFlavobacteriumBacteroides group isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 54, 2335–2341.[CrossRef]
    [Google Scholar]
  7. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  8. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  9. Copa-Patiño, J. L., Arenas, M., Soliveri, J., Sánchez-Porro, C. & Ventosa, A. ( 2008; ). Algoriphagus hitonicola sp. nov., isolated from an athalassohaline lagoon. Int J Syst Evol Microbiol 58, 424–428.[CrossRef]
    [Google Scholar]
  10. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package) version 3.5.1. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  11. Groth, I., Schumann, P., Rainey, F. A., Martin, K., Schuetze, B. & Augsten, K. ( 1997; ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47, 1129–1133.[CrossRef]
    [Google Scholar]
  12. Guindon, S. & Gascuel, O. ( 2003; ). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52, 696–704.[CrossRef]
    [Google Scholar]
  13. Joshi, A. A., Kanekar, P. P., Kelkar, A. S., Shouche, Y. S., Vani, A. A., Borgave, S. B. & Sarnaik, S. S. ( 2008; ). Cultivable bacterial diversity of alkaline Lonar lake, India. Microb Ecol 55, 163–172.[CrossRef]
    [Google Scholar]
  14. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  15. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  16. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt, E. & Goodfellow, M.. Chichester. : Wiley.
    [Google Scholar]
  17. Lányí, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  18. Liu, Y., Li, H., Jiang, J. T., Liu, Y. H., Song, X. F., Xu, C. J. & Liu, Z. P. ( 2009; ). Algoriphagus aquatilis sp. nov., isolated from a freshwater lake. Int J Syst Evol Microbiol 59, 1759–1763.[CrossRef]
    [Google Scholar]
  19. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  20. Nedashkovskaya, O. I., Vancanneyt, M., Van Trappen, S., Vandemeulebroecke, K., Lysenko, A. M., Rohde, M., Falsen, E., Frolova, G. M., Mikhailov, V. V. & Swings, J. ( 2004; ). Description of Algoriphagus aquimarinus sp. nov., Algoriphagus chordae sp. nov. and Algoriphagus winogradskyi sp. nov., from sea water and algae, transfer of Hongiella halophila Yi and Chun 2004 to the genus Algoriphagus as Algoriphagus halophilus comb. nov. and emended descriptions of the genera Algoriphagus Bowman et al. 2003 and Hongiella Yi and Chun 2004. Int J Syst Evol Microbiol 54, 1757–1764.[CrossRef]
    [Google Scholar]
  21. Nedashkovskaya, O. I., Kim, S. B., Lee, M. S., Park, M. S., Lee, K. H., Lysenko, A. M., Oh, H. W., Mikhailov, V. V. & Bae, K. S. ( 2005; ). Cyclobacterium amurskyense sp. nov., a novel marine bacterium isolated from sea water. Int J Syst Evol Microbiol 55, 2391–2394.[CrossRef]
    [Google Scholar]
  22. Nedashkovskaya, O. I., Kim, S. B., Vancanneyt, M., Lysenko, A. M., Shin, D. S., Park, M. S., Lee, K. H., Jung, W. J., Kalinovskaya, N. I. & other authors ( 2006; ). Echinicola pacifica gen. nov., sp. nov., a novel flexibacterium isolated from the sea urchin Strongylocentrotus intermedius. Int J Syst Evol Microbiol 56, 953–958.[CrossRef]
    [Google Scholar]
  23. Nedashkovskaya, O. I., Kim, S. B., Kwon, K. K., Shin, D. S., Luo, X., Kim, S. J. & Mikhailov, V. V. ( 2007; ). Proposal of Algoriphagus vanfongensis sp. nov., transfer of members of the genera Hongiella Yi and Chun 2004 emend. Nedashkovskaya et al. 2004 and Chimaereicella Tiago et al. 2006 to the genus Algoriphagus, and emended description of the genus Algoriphagus Bowman et al. 2003 emend. Nedashkovskaya et al. 2004. Int J Syst Evol Microbiol 57, 1988–1994.[CrossRef]
    [Google Scholar]
  24. Raj, H. D. & Maloy, S. R. ( 1990; ). Proposal of Cyclobacterium marinus gen. nov., comb. nov., for a marine bacterium previously assigned to the genus Flectobacillus. Int J Syst Bacteriol 40, 337–347.[CrossRef]
    [Google Scholar]
  25. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  26. Sly, L. I., Blackall, L. L., Kraat, P. C., Tao, T.-S. & Sangkhobol, V. ( 1986; ). The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J Microbiol Methods 5, 139–156.[CrossRef]
    [Google Scholar]
  27. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R.. Washington, DC. : American Society for Microbiology.
    [Google Scholar]
  28. Thompson, J. D., Higgins, D. G., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  29. Van Trappen, S., Vandecandelaere, I., Mergaert, J. & Swings, J. ( 2004; ). Algoriphagus antarcticus sp. nov., a novel psychrophile from microbial mats in Antarctic lakes. Int J Syst Evol Microbiol 54, 1969–1973.[CrossRef]
    [Google Scholar]
  30. Ying, J. Y., Wang, B. J., Yang, S. S. & Liu, S. J. ( 2006; ). Cyclobacterium lianum sp. nov., a marine bacterium isolated from sediment of an oilfield in the South China Sea, and emended description of the genus Cyclobacterium. Int J Syst Evol Microbiol 56, 2927–2930.[CrossRef]
    [Google Scholar]
  31. Yoon, J. H., Kang, S. J. & Oh, T. K. ( 2005; ). Algoriphagus locisalis sp. nov., isolated from a marine solar saltern. Int J Syst Evol Microbiol 55, 1635–1639.[CrossRef]
    [Google Scholar]
  32. Yoon, J. H., Lee, M. H., Kang, S. J. & Oh, T. K. ( 2006; ). Algoriphagus terrigena sp. nov., isolated from soil. Int J Syst Evol Microbiol 56, 777–780.[CrossRef]
    [Google Scholar]
  33. ZoBell, C. E. ( 1941; ). Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 4, 42–75.
    [Google Scholar]
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