The taxonomic position of three strains from water, soil and lettuce samples was studied by using a polyphasic taxonomic approach. The strains were reported to lack the virulence-encoding genes and in a previous study. Controversially, API 20 E and some other phenotypic tests suggested that the strains belong to , which prompted this polyphasic taxonomic study. In both the phylogenetic analyses of four housekeeping genes (, , and ) and numerical analyses of dIII and RI ribopatterns, the strains formed a separate group within the genus . Analysis of the 16S rRNA gene sequences showed that the strains were related to and , but DNA–DNA hybridization analysis differentiated them from these species. Based on the results of the phylogenetic and DNA–DNA hybridization analyses, a novel species, sp. nov., is proposed. The type strain is ÅYV7.1KOH2 ( = DSM 22769  = LMG 25369).


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vol. , part 10, pp. 2363–2367

GenBank accession numbers of , , and gene sequences used in this study

dIII and RI ribopatterns and numerical analysis of the patterns presented as a dendrogram

Transmission electron micrograph of negatively stained cells of sp. nov. ÅYV7.1KOH2

Maximum-parsimony tree based on concatenated , , and gene sequences of sp. nov. and type and reference strains

Maximum-parsimony tree based on 16S rRNA gene sequences of sp. nov. and type and reference strains

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