The taxonomic position of three strains from water, soil and lettuce samples was studied by using a polyphasic taxonomic approach. The strains were reported to lack the virulence-encoding genes and in a previous study. Controversially, API 20 E and some other phenotypic tests suggested that the strains belong to , which prompted this polyphasic taxonomic study. In both the phylogenetic analyses of four housekeeping genes (, , and ) and numerical analyses of dIII and RI ribopatterns, the strains formed a separate group within the genus . Analysis of the 16S rRNA gene sequences showed that the strains were related to and , but DNA–DNA hybridization analysis differentiated them from these species. Based on the results of the phylogenetic and DNA–DNA hybridization analyses, a novel species, sp. nov., is proposed. The type strain is ÅYV7.1KOH2 ( = DSM 22769  = LMG 25369).

This study was supported by the:
  • Academy of Finland
  • ELVIRA programme (Award 1118602 and 1117921)

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