1887

Abstract

The taxonomic position of three strains from water, soil and lettuce samples was studied by using a polyphasic taxonomic approach. The strains were reported to lack the virulence-encoding genes and in a previous study. Controversially, API 20 E and some other phenotypic tests suggested that the strains belong to , which prompted this polyphasic taxonomic study. In both the phylogenetic analyses of four housekeeping genes (, , and ) and numerical analyses of dIII and RI ribopatterns, the strains formed a separate group within the genus . Analysis of the 16S rRNA gene sequences showed that the strains were related to and , but DNA–DNA hybridization analysis differentiated them from these species. Based on the results of the phylogenetic and DNA–DNA hybridization analyses, a novel species, sp. nov., is proposed. The type strain is ÅYV7.1KOH2 ( = DSM 22769  = LMG 25369).

Funding
This study was supported by the:
  • Academy of Finland
  • ELVIRA programme (Award 1118602 and 1117921)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.019984-0
2011-10-01
2021-04-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/10/2363.html?itemId=/content/journal/ijsem/10.1099/ijs.0.019984-0&mimeType=html&fmt=ahah

References

  1. Atlas R. M., Snyder J. W. 2006 Handbook of Media for Clinical Microbiology, 2nd edn. Boca Raton, FL: CRC Press; [CrossRef]
    [Google Scholar]
  2. Jakava-Viljanen M., Murros A., Palva A., Björkroth K. J. 2008; Lactobacillus sobrius Konstantinov et al. 2006 is a later synonym of Lactobacillus amylovorus Nakamura 1981. Int J Syst Evol Microbiol 58:910–913 [CrossRef][PubMed]
    [Google Scholar]
  3. Kotetishvili M., Kreger A., Wauters G., Morris J. G. Jr, Sulakvelidze A., Stine O. C. 2005; Multilocus sequence typing for studying genetic relationships among Yersinia species. J Clin Microbiol 43:2674–2684 [CrossRef][PubMed]
    [Google Scholar]
  4. Merhej V., Adékambi T., Pagnier I., Raoult D., Drancourt M. 2008; Yersinia massiliensis sp. nov., isolated from fresh water. Int J Syst Evol Microbiol 58:779–784 [CrossRef][PubMed]
    [Google Scholar]
  5. Niskanen T., Laukkanen R., Murros A., Björkroth J., Skurnik M., Korkeala H., Fredriksson-Ahomaa M. 2009; Characterisation of non-pathogenic Yersinia pseudotuberculosis-like strains isolated from food and environmental samples. Int J Food Microbiol 129:150–156 [CrossRef][PubMed]
    [Google Scholar]
  6. Pitcher D. G., Saunders N. A., Owen R. J. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156 [CrossRef]
    [Google Scholar]
  7. Sprague L. D., Scholz H. C., Amann S., Busse H.-J., Neubauer H. 2008; Yersinia similis sp. nov.. Int J Syst Evol Microbiol 58:952–958 [CrossRef][PubMed]
    [Google Scholar]
  8. Stackebrandt E., Liesack W. 1993; Nucleic acids and classification. In Handbook of New Bacterial Systematics pp. 152–194 Edited by Goodfellow M., O’Donnell A. G. London, UK: Academic Press;
    [Google Scholar]
  9. Xu H. X., Kawamura Y., Li N., Zhao L., Li T. M., Li Z. Y., Shu S., Ezaki T. 2000; A rapid method for determining the G+C content of bacterial chromosomes by monitoring fluorescence intensity during DNA denaturation in a capillary tube. Int J Syst Evol Microbiol 50:1463–1469 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.019984-0
Loading
/content/journal/ijsem/10.1099/ijs.0.019984-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error