1887

Abstract

Strain Eg1, an anaerobic, Gram-stain-positive, non-motile and non-spore-forming bacterium, was isolated from human faeces. The optimal temperature for growth was 37 °C and tests for oxidase and catalase activities gave negative results. Fructose-6-phosphate phosphoketolase activity was detected. Acid was produced during fermentation of several substrates, including glucose. The end products of glucose fermentation were acetic acid and lactic acid, which were produced in a molar ratio of 1.76 : 1 (approximately 3 : 2). The G+C content was 57.8 mol%. Comparative analysis of 16S rRNA gene sequences showed that strain Eg1 was closely related to YIT 4011 (98.36 % 16S rRNA gene sequence similarity) and JCM 8222 (97.93 %) and analysis of sequences showed that strain Eg1 was closely related to . JCM 1275 (99.35 % sequence similarity) and . JCM 8222 (92.13 %). However, despite these degrees of similarity being high enough for strain Eg1 to be included at the same species level as . and . (96.5–100 % for the genus ), the isolate could be distinguished from . KCTC 3216 and . KCTC 3425 by low levels of DNA–DNA relatedness (41 and 17 %, respectively). Based on phenotypic, genotypic and phylogenetic analyses, we propose that strain Eg1 is classified in a novel species, sp. nov. The type strain is Eg1 (=KCTC 5756 =JCM 15918).

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2010-12-01
2019-09-21
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vol. , part 12, pp. 2823 - 2827

Neighbour-joining phylogenetic tree based on partial sequences (positions 304–831 of the sequence of ) showing the phylogenetic positions of strain Eg1 and type strains of the genus . [PDF](43 KB)



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