1887

Abstract

A Gram-negative, non-motile, aerobic bacterial strain, designated MJ20, was isolated from farm soil near Daejeon (South Korea) and was characterized taxonomically by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ20 belongs to the family , class , and was related most closely to DSM 18053 (98.9 % sequence similarity), JCM 14200 (98.0 %) and KCTC 12589 (96.4 %). The G+C content of the genomic DNA of strain MJ20 was 48.5 mol%. The detection of MK-7 as the predominant menaquinone and a fatty acid profile with summed feature 4 (C 7 and/or iso-C 2-OH), iso-C, C and C 5 as major components supported the affiliation of strain MJ20 to the genus . The new isolate exhibited relatively low levels of DNA–DNA relatedness with respect to DSM 18053 (mean± of three determinations, 47±7 %) and JCM 14200 (38±8 %). On the basis of its phenotypic and genotypic properties together with phylogenetic distinctiveness, strain MJ20 (=KCTC 22481 =JCM 16232) should be classified in the genus as the type strain of a novel species, for which the name sp. nov. is proposed.

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2010-11-01
2024-11-05
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