1887

Abstract

An obligately anaerobic, methanogenic archaeon, strain HC, was isolated from soil of the Zoige wetland on the Tibetan plateau, China. The strain was isolated through construction of an artificial butyrate-degrading consortium in co-culture with a syntrophic bacterium, ‘ subsp. ’ JCM 13344. Cells of strain HC were irregular coccoids, 0.8–2 μm in diameter, that occurred singly and utilized only H/CO for growth and methane production. Growth occurred at 18–45 °C (optimum around 37 °C). The pH for growth was 5.0–8.5 (optimal growth around pH 6.6). The G+C content of the genomic DNA was 60.2 mol%. 16S rRNA gene sequence analysis indicated that strain HC was affiliated to the genus , with sequence similarities of 94.8–97.2 % to existing members. However, strain HC was distinguished from described species by not using formate for growth or methane formation and not requiring acetate as a growth factor. On the basis of phylogenetic analysis and phenotypic characteristics, the novel species sp. nov. is proposed, with strain HC (=CGMCC 1.5146 =JCM 16311) as the type strain.

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2010-09-01
2019-10-16
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vol. , part 9, pp. 2165 - 2169

Effects of temperature (a), pH (b) and salinity (c) on growth of strain HC on H /CO . Means were calculated from triplicate cultures.



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