1887

Abstract

An aerobic, actinobacterial strain with rod-shaped spores, EUM 221, which was isolated from the surface-sterilized stem of a grey box tree (), is described. Phylogenetic evaluation based on 16S rRNA gene sequence similarity showed that this isolate belongs to the family , with the closest neighbour being 6330 (98.7 %). The level of 16S rRNA gene sequence similarity between the isolate and species of the genus with validly published names ranged from 95 to 98 %. Chemotaxonomic data (-diaminopimelic acid; major menaquinone MK-8(H); major fatty acid iso-C) confirmed the affiliation of strain EUM 221 to the genus . The results of the phylogenetic analysis, including physiological and biochemical studies in combination with DNA–DNA hybridization, allowed the genotypic and phenotypic differentiation of strain EUM 221 from the closest described species. Therefore, this strain represented a novel species and the name proposed is sp. nov. The type strain is EUM 221 (=DSM 45352 =ACM 5286).

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2010-12-01
2019-09-20
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[PDF file of Supplementary Figs S1 and S2](57 KB)

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Scanning electron micrograph of growth of strain EUM 221 grown on ISP 4 for 21 days at 27 °C. Bar, 1 µm.

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