1887

Abstract

A short coccoid- to rod-shaped, motile, mesophilic actinobacterium, strain MSL-22, was isolated from soil on Bigeum Island, Korea. A polyphasic study was undertaken to establish the taxonomic position of this strain. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MSL-22 formed an evolutionary lineage within the radiation of the genus . In particular, it formed a monophyletic lineage with KCTC 9134 with which it shared the highest sequence similarity of about 97.3%. However, DNA–DNA relatedness demonstrated that strain MSL-22 was distinct from its closest phylogenetic neighbours. The cell-wall peptidoglycan of strain MSL-22 contained -diaminopimelic acid. The predominant menaquinone was MK-8(H). Strain MSL-22 had a cellular fatty acid profile containing straight-chain, branched, unsaturated and 10-methyl fatty acids, with iso-C as the major fatty acid. The DNA G+C content of the strain was 68.7 mol%. On the basis of phenotypic and phylogenetic evidence, the strain is separate from previously described members of the genus and represents a novel species in this genus, for which the name sp. nov. is proposed. The type strain is MSL-22 (=DSM 19432=KCTC 19310).

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2010-10-01
2019-10-16
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vol. , part 10, pp. 2288 - 2292

Scanning electron micrograph of cells of strain MSL-22 grown on R2A agar for 3 days at 28°C. Bar, 2.0 µm.



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