1887

Abstract

A novel gammaproteobacterium, strain CN3, was isolated from the faeces of wild boar. Strain CN3 was facultatively anaerobic and appeared coccoid or rod-shaped. The partial 16S rRNA gene sequence determined for strain CN3 suggested a distant relationship with members of the orders ‘’ and . The gene sequence showed highest similarity (90.3 %) with DSM 2777, a member of the family . The closest relatives outside the order ‘’ according to 16S rRNA gene sequence analysis were members of the order with 88.7 % similarity ( NCTC 9380 and NCTC 4189). In contrast to most members of the order ‘’, strain CN3 was oxidase-positive. The pattern of fatty acids, in particular the high relative abundance of C 7 (38.5 %), was clearly distinct from the conserved pattern found for members of the order . RI ribotyping of strain CN3 yielded no significant similarity to existing database entries. The major ubiquinone of strain CN3 was Q-8. The DNA G+C content was 36.4 mol%. Strain CN3 hosted a phage and secreted considerable amounts of three proteins into the culture supernatant. A spontaneous mutant of strain CN3 was isolated which formed long filaments. Microscopic studies revealed the presence of a capsule that the mutant strain was unable to partition after cell division. Strain CN3 thus represents a novel species within a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is CN3 (=DSM 22228=CCUG 57622). Classification of the novel species to the family and order level will require further investigations.

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2010-11-01
2020-01-24
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vol. , part 11, pp. 2601 - 2605

Combined PDF file containing:

. Growth of strain CN3 on cefsulodin-irgasan-novobiocin (CIN) agar after incubation for 40 hours at 27°C. Images demonstrating the motility of strain CN3 . Phase contrast microscopy, Gram staining, transmission electron micrograph and scanning electron photomicrograph of the filamentous mutant of strain CN3 . Temperature-dependence of growth of strain CN3 in 2×YT broth under aerobic and anaerobic conditions. Coomassie-stained denaturing SDS-PAGE analysis of secreted proteins of strain CN3 .

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