1887

Abstract

A novel gammaproteobacterium, strain CN3, was isolated from the faeces of wild boar. Strain CN3 was facultatively anaerobic and appeared coccoid or rod-shaped. The partial 16S rRNA gene sequence determined for strain CN3 suggested a distant relationship with members of the orders ‘’ and . The gene sequence showed highest similarity (90.3 %) with DSM 2777, a member of the family . The closest relatives outside the order ‘’ according to 16S rRNA gene sequence analysis were members of the order with 88.7 % similarity ( NCTC 9380 and NCTC 4189). In contrast to most members of the order ‘’, strain CN3 was oxidase-positive. The pattern of fatty acids, in particular the high relative abundance of C 7 (38.5 %), was clearly distinct from the conserved pattern found for members of the order . RI ribotyping of strain CN3 yielded no significant similarity to existing database entries. The major ubiquinone of strain CN3 was Q-8. The DNA G+C content was 36.4 mol%. Strain CN3 hosted a phage and secreted considerable amounts of three proteins into the culture supernatant. A spontaneous mutant of strain CN3 was isolated which formed long filaments. Microscopic studies revealed the presence of a capsule that the mutant strain was unable to partition after cell division. Strain CN3 thus represents a novel species within a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is CN3 (=DSM 22228=CCUG 57622). Classification of the novel species to the family and order level will require further investigations.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.019026-0
2010-11-01
2024-11-10
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/11/2601.html?itemId=/content/journal/ijsem/10.1099/ijs.0.019026-0&mimeType=html&fmt=ahah

References

  1. Christensen H., Kuhnert P., Busse H. J., Frederiksen W. C., Bisgaard M. 2007; Proposed minimal standards for the description of genera, species and subspecies of the Pasteurellaceae . Int J Syst Evol Microbiol 57:166–178 [CrossRef]
    [Google Scholar]
  2. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian protein metabolism pp 21–132 Edited by Munro H. N. New York: Academic press;
    [Google Scholar]
  3. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685 [CrossRef]
    [Google Scholar]
  4. Mattick J. S. 2002; Type IV pili and twitching motility. Annu Rev Microbiol 56:289–314 [CrossRef]
    [Google Scholar]
  5. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  6. Mutters R., Mouahid M., Engelhard E., Mannheim W. 1993; Characterization of the family Pasteurellaceae on the basis of cellular lipids and carbohydrates. Zentralbl Bakteriol 279:104–113 [CrossRef]
    [Google Scholar]
  7. NCCLS 1997 Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. Approved standard M7–A4, 4th edn. Wayne, Pa: National Committee for Clinical Laboratory Standards;
    [Google Scholar]
  8. NCCLS 1999; ). . Performance Standards for Antimicrobial Susceptibility Testing. Ninth informational supplement, M100–S9. 1–1-1999 Wayne Pa: National Committee for Clinical Laboratory Standards;
    [Google Scholar]
  9. Pruesse E., Quast C., Knittel K., Fuchs B. M., Ludwig W., Peplies J., Glöckner F. O. 2007; SILVA: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with ARB. Nucleic Acids Res 35:7188–7196 [CrossRef]
    [Google Scholar]
  10. Schiemann D. A. 1979; Synthesis of a selective agar medium for Yersinia enterocolitica . Can J Microbiol 25:1298–1304 [CrossRef]
    [Google Scholar]
  11. Trček J., Wilharm G., Jacobi C. A., Heesemann J. 2002; Yersinia enterocolitica YopQ: strain-dependent cytosolic accumulation and post-translational secretion. Microbiology 148:1457–1465
    [Google Scholar]
  12. Trebesius K., Harmsen D., Rakin A., Schmelz J., Heesemann J. 1998; Development of rRNA-targeted PCR and in situ hybridization with fluorescently labelled oligonucleotides for detection of Yersinia species. J Clin Microbiol 36:2557–2564
    [Google Scholar]
  13. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. 1991; 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703
    [Google Scholar]
  14. Ziesché K., Reissbrodt R., Rische H. 1985; A bile-chrysoidine-glycerol culture medium and its use in the diagnosis of gram-negative aerobic bacteria, especially Enterobacteriaceae . Z Gesamte Hyg 31:516–518
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.019026-0
Loading
/content/journal/ijsem/10.1099/ijs.0.019026-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error