1887

Abstract

A novel, non-pigmented, rod-shaped, Gram-negative strain was isolated from mesotrophic lake water in Zealand, Denmark. Phylogenetic analysis of the 16S rRNA gene sequence of the bacterium, designated strain 389, indicated that the strain belonged to the genus and formed a monophyletic group with DS-28 (99.1 % nucleotide similarity); it was less related to ATCC 19706 (96.9 % similarity) and LMG 24504 (96.8 % similarity). Hybridization of DNA from strain 389 and demonstrated a reassociation of 50.6±9.6 %. The DNA G+C content of strain 389 was 61.2 mol%. The fatty acid profile of the strain differed from those of the other strains representing the genus by a high content of C 7 and/or iso-C 2-OH (71.6 %) and a lower content of C. Strain 389 was capable of degrading peptidoglycan and had chitinase and lysozyme activities, possibly associated with the degradation of peptidoglycan, and had capacity for degradation of several other polymer compounds. Based on phenotypic and genotypic characteristics, strain 389 represents a novel species, for which we propose the name sp. nov. The type strain is 389 (=DSM 21247 =LMG 25302).

Keyword(s): PG, peptidoglycan
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2010-10-01
2020-01-28
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vol. , part 10, pp. 2467 - 2472

Cultures of strain 389 grown on 1/25 TSB agar without PG (left) and with PG (right). Opaque zone surrounding the inoculum indicates degradation of PG (right).



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