1887

Abstract

Two Gram-negative-staining, aerobic, non-motile, rod-shaped bacteria, designated strains FFA1 and FFA3, and belonging to the class were isolated from the gastrointestinal tract of adult flesh flies (Diptera: Sarcophagidae). Phylogenetic analysis of 16S rRNA gene sequence data placed these two strains within the genus with similarities of 98.6 % (FFA1) and 99.35 % (FFA3) to L1/68. The level of gene sequence similarity between strains FFA1 and FFA3 was 99 %, 97.15 % and 78.1 % based on the 16S rRNA, 23S rRNA and gene sequences, respectively. Strains FFA1 and FFA3 shared 24 % DNA–DNA relatedness. DNA–DNA hybridization revealed a very low level of relatedness between the novel strains (22 % for strain FFA1 and 44 % for strain FFA3) and . L1/68 genomic DNA. The respiratory quinone was Q-8 in both novel strains. The DNA G+C contents were 41.1 mol% and 40.1 mol% for strains FFA1 and FFA3, respectively. The cell membrane of both strains consisted of phosphatidylglycerol, phosphatidylethanolamine, phospholipids and aminophospholipid. The major fatty acids for both strains were C, summed feature 8 (Cω7 and/or Cω6), CyCω8 and C. The results of DNA–DNA hybridization between the two new strains and L1/68, in combination with phylogenetic, chemotaxonomic, biochemical and electron microscopic data, demonstrated that strains FFA1 and FFA3 represented two novel species of the genus for which the names sp. nov. (type strain FFA1 = DSM 22309 = KCTC 22643 = NCIM 5325) and sp. nov. (type strain FFA3 = DSM 22310 = KCTC 22644 = NCIM 5326) are proposed.

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2011-06-01
2020-01-27
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vol. , part 6, pp. 1360 - 1369

Longitudinal section of cells of strain FFA1 as depicted by TEM. Cells were grown on MH agar plates for 24 h at 37 °C.

(a) Maximum-parsimony tree based on 16S rRNA gene sequences showing the phylogenetic position of strains FFA1 and FFA3 among members of the class . (b). Maximum-parsimony tree based on 23S rRNA gene sequences showing the phylogenetic position of strains FFA1 and FFA3 among . (c). Maximum-parsimony tree based on amino acid sequences showing the phylogenetic position of strains FFA1 and FFA3 among members of the class .

Polar lipid profiles of strains FFA1 (a) and FFA3 (b) after two-dimensional silica gel TLC.

ERIC and REP PCR genomic fingerprints of the novel strains.

Results of GN2 Micro Plates (Biolog).

Response to antimicrobials.

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