1887

Abstract

A basidiomycetous yeast, strain CRUB 1032, which formed salmon-pink colonies, was isolated from glacial meltwater in Patagonia, Argentina. Morphological, physiological and biochemical characterization indicated that this strain belonged to the genus . Molecular taxonomic analysis based on the 26S rDNA D1/D2 domain and internal transcribed spacer region sequences showed that strain CRUB 1032 represents an undescribed yeast species, for which the name sp. nov. is proposed (type strain is CRUB 1032=CBS 10797=JCM 15319). Phylogenetic analysis showed that was the closest known species, which, together with , formed a separate cluster related to the clade within the Cystobasidiomycetes. Additional Patagonian yeast isolates of the class Cystobasidiomycetes are also investigated in the present work.

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2010-09-01
2021-03-07
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References

  1. Bai F.-Y., Cai Y., Wang Q.-M., Ohkubo H. 2004; Rhodotorula oryzae sp. nov., a novel basidiomycetous yeast species isolated from paddy rice. Antonie van Leeuwenhoek 86:295–299 [CrossRef]
    [Google Scholar]
  2. Bauer R., Begerow D., Sampaio J. P., Weiß M., Oberwinkler F. 2006; The simple-septate basidiomycetes: a synopsis. Mycol Prog 5:41–66 [CrossRef]
    [Google Scholar]
  3. de García V., Brizzio S., Libkind D., Buzzini P., van Broock M. 2007; Biodiversity of cold-adapted yeasts from glacial meltwater rivers in Patagonia, Argentina. FEMS Microbiol Ecol 59:331–341 [CrossRef]
    [Google Scholar]
  4. Fell J. W., Hunter I. L., Tallman A. S. 1973; Marine basidiomycetous yeasts ( Rhodosporidium spp. n.) with tetrapolar and multiple allelic bipolar mating systems. Can J Microbiol 19:643–657 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  6. Fonseca A. 1992; Utilization of tartaric acid and related compounds by yeasts: taxonomic implications. Can J Microbiol 38:1242–1251 [CrossRef]
    [Google Scholar]
  7. Gadanho M., Almeida J. M. G. C. F., Sampaio J. P. 2003; Assessment of yeast diversity in a marine environment in the south of Portugal by microsatellite-primed PCR. Antonie van Leeuwenhoek 84:217–227 [CrossRef]
    [Google Scholar]
  8. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  9. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  10. Libkind D. 2006; Levaduras carotenogénicas de ambientes acuáticos de la Patagonia noroccidental Argentina. Aplicaciones biotecnológicas . PhD thesis Universidad Nacional de Tucumán; Argentina: (in Spanish
  11. Libkind D., Brizzio S., Ruffini A., Gadanho M., van Broock M. R., Sampaio J. P. 2003; Molecular characterization of carotenogenic yeasts from aquatic environments in Patagonia, Argentina. Antonie van Leeuwenhoek 84:313–322 [CrossRef]
    [Google Scholar]
  12. Libkind D., Gadanho M., van Broock M. R., Sampaio J. P. 2005a; Sporidiobolus longiusculus sp. nov. and Sporobolomyces patagonicus sp. nov., novel yeasts of the Sporidiobolales isolated from aquatic environments in Patagonia, Argentina. Int J Syst Evol Microbiol 55:503–509 [CrossRef]
    [Google Scholar]
  13. Libkind D., Sommaruga R., Zagarese H., van Broock M. R. 2005b; Mycosporines in carotenogenic yeasts. Syst Appl Microbiol 28:749–754 [CrossRef]
    [Google Scholar]
  14. Libkind D., Gadanho M., van Broock M., Sampaio J. P. 2009a; Cystofilobasidium lacus-mascardii sp. nov., a basidiomycetous yeast species isolated from aquatic environments of the Patagonian Andes and Cystofilobasidium macerans sp. nov., the sexual stage of Cryptococcus macerans . Int J Syst Evol Microbiol 59:622–630 [CrossRef]
    [Google Scholar]
  15. Libkind D., Moliné M., Sampaio J. P., van Broock M. 2009b; Yeasts from high altitude lakes: influence of UV radiation. FEMS Microbiol Ecol 69:353–362 [CrossRef]
    [Google Scholar]
  16. Nagahama T., Hamamoto M., Nakase T., Horikoshi K. 2001; Rhodotorula lamellibrachii sp. nov., a new yeast species from a tubeworm collected at the deep-sea floor in Sagami Bay and its phylogenetic analysis. Antonie van Leeuwenhoek 80:317–323 [CrossRef]
    [Google Scholar]
  17. Rayner R. W. 1970 A Mycological Colour Chart Kew, UK: Commonwealth Mycological Institute and British Mycological Society;
    [Google Scholar]
  18. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  19. Sampaio J. P. 1999; Utilization of low molecular weight aromatic compounds by heterobasidiomycetous yeasts: taxonomic implications. Can J Microbiol 45:491–512 [CrossRef]
    [Google Scholar]
  20. Sampaio J. P., Gadanho M., Santos S., Duarte F., Pais C., Fonseca A., Fell J. W. 2001; Polyphasic taxonomy of the genus Rhodosporidium : R. kratochvilovae and related anamorphic species. Int J Syst Evol Microbiol 51:687–697
    [Google Scholar]
  21. Tamura K., Dudley J., Nei M., Kumar S. 2007; mega4: Molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24:1596–1599 [CrossRef]
    [Google Scholar]
  22. Yarrow D. 1998; Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn. pp 77–100 Edited by Kurtzman C. P., Fell J. W. Amsterdam: Elsevier;
    [Google Scholar]
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