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Abstract

A novel Gram-stain-positive, coccoid actinobacterium, designated strain Sco-D01, was isolated from volcanic ash collected from Oreum (a parasitic volcanic cone) on Jeju Island, Republic of Korea. Cells were aerobic, oxidase-negative and catalase-positive. Colonies were vivid yellow, circular, smooth and convex. The diagnostic diamino acid in the cell wall was -diaminopimelic acid. The predominant menaquinone was MK-8(H). The polar lipids were phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and an unknown phospholipid. The fatty acid profile was represented by large amounts of saturated, unsaturated, 10-methyl and hydroxyl components. The DNA G+C content of strain Sco-D01 was 72.0 mol%. 16S rRNA gene sequence analysis revealed that strain Sco-D01 belonged to the family and formed a distinct sublineage within the radiation of the genus . The level of DNA–DNA relatedness between strain Sco-D01 and its closest phylogenetic relative, DSM 12652, was 30.2 % (35.4 % in duplicate measurements). On the basis of phenotypic and DNA–DNA hybridization data, strain Sco-D01 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Sco-D01 (=KCTC 19597=DSM 22127).

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2010-09-01
2019-10-20
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vol. , part 9, pp. 2135–2139

Cellular fatty acid profiles of strain Sco-D01 and the type strains of recognized species [ PDF] (68 KB)



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