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Abstract

A non-motile, rod-shaped and aerobic marine bacterium, designated strain KYW314, was isolated from seawater collected from the South Sea, Republic of Korea. Cells were Gram-negative, catalase- and oxidase-positive and had proteolytic activity. The major fatty acids were iso-C (17.2 %), C (11.1 %), iso-C (10.5 %) and C 7 (10.0 %). The DNA G+C content was 56.4 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain KYW314 formed a lineage within the genus (95.4–97.3 % sequence similarity) and a distinct branch within the clade containing PIT1 and c121. Phenotypic characteristics could distinguish strain KYW314 from members of the genus On the basis of the data presented, strain KYW314 represents a novel species, for which the name sp. nov. is proposed. The type strain is KYW314 (=KCTC 22740 =JCM 16344).

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2010-09-01
2019-10-20
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References

  1. Barrow, G. I. & Feltham, R. K. A. ( 1993; ). Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge. : Cambridge University Press.
    [Google Scholar]
  2. Chun, J. ( 1995; ). Computer-assisted classification and identification of actinomycetes. PhD thesis, University of Newcastle, Newcastle, UK.
  3. Chun, J. & Goodfellow, M. ( 1995; ). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45, 240–245.[CrossRef]
    [Google Scholar]
  4. Chun, J., Bae, K. S., Moon, E. Y., Jung, S. O., Lee, H. K. & Kim, S. J. ( 2000; ). Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol 50, 1909–1913.
    [Google Scholar]
  5. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  6. CLSI ( 2003; ). Performance standards for antimicrobial disk susceptibility tests, 8th edn. Approved Standard M2-A8. Wayne, PA: Clinical and Laboratory Standards Institute.
  7. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  8. Felsenstein, J. ( 1993; ). phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  9. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  10. Fitch, W. M. & Margoliash, E. ( 1967; ). Construction of phylogenetic trees. Science 155, 279–284.[CrossRef]
    [Google Scholar]
  11. Hu, Z. Y. & Li, Y. ( 2007; ). Pseudidiomarina sediminum sp. nov., a marine bacterium isolated from coastal sediments of Luoyuan Bay in China. Int J Syst Evol Microbiol 57, 2572–2577.[CrossRef]
    [Google Scholar]
  12. Ivanova, E. P., Romanenko, L. A., Chun, J., Matte, M. H., Matte, G. R., Mikhailov, V. V., Svetashev, V. I., Huq, A., Maugel, T. & Colwell, R. R. ( 2000; ). Idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including descriptions of two species, Idiomarina abyssalis sp. nov. and Idiomarina zobellii sp. nov. Int J Syst Evol Microbiol 50, 901–907.[CrossRef]
    [Google Scholar]
  13. Ivanova, E. P., Flavier, S. & Christen, R. ( 2004; ). Phylogenetic relationships among marine Alteromonas-like proteobacteria: emended description of the family Alteromonadaceae and proposal of Pseudoalteromonadaceae fam. nov., Colwelliaceae fam. nov., Shewanellaceae fam. nov., Moritellaceae fam. nov., Ferrimonadaceae fam. nov., Idiomarinaceae fam. nov. and Psychromonadaceae fam. nov. Int J Syst Evol Microbiol 54, 1773–1788.[CrossRef]
    [Google Scholar]
  14. Jean, W. D., Shieh, W. Y. & Chiu, H.-H. ( 2006; ). Pseudidiomarina taiwanensis gen. nov., sp. nov., a marine bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the family Idiomarinaceae. Int J Syst Evol Microbiol 56, 899–905.[CrossRef]
    [Google Scholar]
  15. Jean, W. D., Leu, T. Y., Lee, C.-Y., Chiu, T.-J., Lin, S. Y. & Shieh, W. Y. ( 2009; ). Pseudidiomarina marina sp. nov. and Pseudidiomarina tainanensis sp. nov. and reclassification of Idiomarina homiensis and Idiomarina salinarum as Pseudidiomarina homiensis comb. nov. and Pseudidiomarina salinarum comb. nov., respectively. Int J Syst Evol Microbiol 59, 53–59.[CrossRef]
    [Google Scholar]
  16. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by Munro, H. N.. New York. : Academic Press.
    [Google Scholar]
  17. Kovács, N. ( 1956; ). Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178, 703.
    [Google Scholar]
  18. Kwon, S.-W., Kim, B.-Y., Weon, H.-Y., Baek, Y.-K., Koo, B.-S. & Go, S.-J. ( 2006; ). Idiomarina homiensis sp. nov., isolated from seashore sand in Korea. Int J Syst Evol Microbiol 56, 2229–2233.[CrossRef]
    [Google Scholar]
  19. Lemos, M. L., Toranzo, A. E. & Barja, J. L. ( 1985; ). Modified medium for the oxidation-fermentation test in the identification of marine bacteria. Appl Environ Microbiol 49, 1541–1543.
    [Google Scholar]
  20. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  21. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  22. Seldin, L. & Dubnau, D. ( 1985; ). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35, 151–154.[CrossRef]
    [Google Scholar]
  23. Skerman, V. B. D. ( 1967; ). A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore. : Williams & Wilkins.
    [Google Scholar]
  24. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R.. Washington, DC. : American Society for Microbiology.
    [Google Scholar]
  25. Swofford, D. L. ( 1998; ). paup: phylogenetic analysis using parsimony, version 4. Sunderland, MA: Sinauer Associates.
  26. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  27. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  28. Wu, Y.-H., Shen, Y.-Q., Xu, X.-W., Wang, C.-S., Oren, A. & Wu, M. ( 2009; ). Pseudidiomarina donghaiensis sp. nov. and Pseudidiomarina maritima sp. nov., isolated from the East China Sea. Int J Syst Evol Microbiol 59, 1321–1325.[CrossRef]
    [Google Scholar]
  29. Yang, S.-H., Kwon, K. K., Lee, H.-S. & Kim, S.-J. ( 2006; ). Shewanella spongiae sp. nov., isolated from a marine sponge. Int J Syst Evol Microbiol 56, 2879–2882.[CrossRef]
    [Google Scholar]
  30. Yoon, J.-H., Jung, S.-Y., Jung, Y.-T. & Oh, T.-K. ( 2007; ). Idiomarina salinarum sp. nov., isolated from a marine solar saltern in Korea. Int J Syst Evol Microbiol 57, 2503–2506.[CrossRef]
    [Google Scholar]
  31. ZoBell, C. E. ( 1941; ). Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 4, 42–75.
    [Google Scholar]
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vol. , part 9, pp. 2071 - 2075

Biolog GN2 MicroPlate results for sp. nov. KYW314 after incubation at 30°C for 24 h. [ PDF] 41 KB



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