1887

Abstract

A Gram-stain-positive, aerobic, rod-shaped, yellow actinobacterium, designated MNA2, was isolated from a hexachlorocyclohexane-contaminated soil in North India. Strain MNA2 showed 95 % 16S rRNA gene sequence similarity with YIM 70130. Phylogenetic analysis of 16S rRNA gene sequences showed that strain MNA2 belonged to a clade represented by the genus of the family . Strain MNA2 contained anteiso-C, anteiso-C, iso-C and iso-C as the predominant fatty acids and diphosphatidylglycerol, phosphatidylglycerol and two unknown polar lipids. The menaquinones were MK-12, MK-11, MK-13 and MK-10, in an approximate molar ratio of 45 : 40 : 13 : 3, respectively. The DNA G+C content was 65.3 mol%. The peptidoglycan was of the B type of cross-linkage with ornithine as the diagnostic diamino acid. The results of the phylogenetic, phenotypic and chemotaxonomic analysis indicate that strain MNA2 belongs to a previously unrecognized species of the genus , for which the name sp. nov. is proposed. The type strain is MNA2 (=DSM 22422 =CCM 7585).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.017699-0
2010-11-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/11/2634.html?itemId=/content/journal/ijsem/10.1099/ijs.0.017699-0&mimeType=html&fmt=ahah

References

  1. Arden-Jones, M. P., McCarthy, A. J. & Cross, T. ( 1979; ). Taxonomic and serological studies on Micropolyspora faeni and Micropolyspora strains from soil bearing the specific epithet rectivirgula. J Gen Microbiol 115, 343–354.[CrossRef]
    [Google Scholar]
  2. Behrendt, U., Ulrich, A. & Schumann, P. ( 2001; ). Description of Microbacterium foliorum sp. nov. and Microbacterium phyllosphaerae sp. nov., isolated from the phyllosphere of grasses and the surface litter after mulching the sward, and reclassification of Aureobacterium resistens (Funke et al. 1998) as Microbacterium resistens comb. nov. Int J Syst Evol Microbiol 51, 1267–1276.
    [Google Scholar]
  3. Bligh, E. G. & Dyer, W. J. ( 1959; ). A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37, 911–917.[CrossRef]
    [Google Scholar]
  4. Bruns, A., Rohde, M. & Berthe-Corti, L. ( 2001; ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51, 1997–2006.[CrossRef]
    [Google Scholar]
  5. Cashion, P., Holder-Franklin, M. A., McCully, J. & Franklin, M. ( 1977; ). A rapid method for base ratio determination of bacterial DNA. Anal Biochem 81, 461–466.[CrossRef]
    [Google Scholar]
  6. Christensen, W. B. ( 1946; ). Urea decomposition as a means of differentiating Proteus and paracolon cultures from each other and from Salmonella and Shigella types. J Bacteriol 52, 461–466.
    [Google Scholar]
  7. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  8. Collins, M. D., Jones, D. & Kroppenstedt, R. M. ( 1983; ). Reclassification of Brevibacterium imperiale (Steinhaus) and ‘Corynebacterium laevaniformans’ (Dias and Bhat) in a redefined genus Microbacterium (Orla-Jensen), as Microbacterium imperiale comb. nov. and Microbacterium laevaniformans nom. rev., comb. nov. Syst Appl Microbiol 4, 65–78.[CrossRef]
    [Google Scholar]
  9. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London. : Cambridge University Press.
    [Google Scholar]
  10. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef]
    [Google Scholar]
  11. Gupta, S. K., Kumari, R., Prakash, O. & Lal, R. ( 2008; ). Pseudomonas panipatensis sp. nov., isolated from an oil-contaminated site. Int J Syst Evol Microbiol 58, 1339–1345.[CrossRef]
    [Google Scholar]
  12. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by Munro, H. N.. New York. : Academic Press.
    [Google Scholar]
  13. Kim, K. K., Park, H. Y., Park, W., Kim, I. S. & Lee, S.-T. ( 2005; ). Microbacterium xylanilyticum sp. nov., a xylan-degrading bacterium isolated from a biofilm. Int J Syst Evol Microbiol 55, 2075–2079.[CrossRef]
    [Google Scholar]
  14. Kushner, D. J. ( 1957; ). An evaluation of the egg-yolk reaction as a test for lecithinase activity. J Bacteriol 73, 297–302.
    [Google Scholar]
  15. Kuykendall, L. D., Roy, M. A., O'Neill, J. J. & Devine, T. E. ( 1988; ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum. Int J Syst Bacteriol 38, 358–361.[CrossRef]
    [Google Scholar]
  16. Lechevalier, M. P. ( 1968; ). Identification of aerobic actinomycetes of clinical importance. J Lab Clin Med 71, 934–944.
    [Google Scholar]
  17. Li, W.-J., Chen, H.-H., Kim, C.-J., Park, D.-J., Tang, S.-K., Lee, J.-C., Xu, L.-H. & Jiang, C.-L. ( 2005; ). Microbacterium halotolerans sp. nov., isolated from a saline soil in the west of China. Int J Syst Evol Microbiol 55, 67–70.[CrossRef]
    [Google Scholar]
  18. MacKenzie, S. L. ( 1987; ). Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70, 151–160.
    [Google Scholar]
  19. McCarthy, A. J. & Cross, T. ( 1984; ). A taxonomic study of Thermomonospora and other monosporic actinomycetes. J Gen Microbiol 130, 5–25.
    [Google Scholar]
  20. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  21. Miller, L. T. ( 1982; ). Single derivatization method for the routine analysis of whole-cell fatty acid methyl esters, including hydroxyl acids. J Clin Microbiol 16, 584–586.
    [Google Scholar]
  22. Orla-Jensen, S. ( 1919; ). The Lactic Acid Bacteria. Copenhagen. : Host & Sons.
    [Google Scholar]
  23. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  24. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  25. Schumann, P., Kämpfer, P., Busse, H.-J. & Evtushenko, L. I. ( 2009; ). Proposed minimal standards for describing new genera and species of the suborder Micrococcineae. Int J Syst Evol Microbiol 59, 1823–1849.[CrossRef]
    [Google Scholar]
  26. Sharma, P., Raina, V., Kumari, R., Malhotra, S., Dogra, C., Kumari, H., Kohler, H. P., Buser, H. R., Holliger, C. & Lal, R. ( 2006; ). The haloalkane dehalogenase LinB is responsible for beta- and delta-hexachlorocyclohexane transformation in Sphingobium indicum B90A. Appl Environ Microbiol 72, 5720–5727.[CrossRef]
    [Google Scholar]
  27. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R.. Washington, DC. : American Society for Microbiology.
    [Google Scholar]
  28. Takahashi, K. & Nei, M. ( 2000; ). Efficiencies of fast algorithms of phylogenetic inference under the criteria of maximum parsimony, minimum evolution and maximum likelihood when a large number of sequences are used. Mol Biol Evol 17, 1251–1258.[CrossRef]
    [Google Scholar]
  29. Takeuchi, M. & Hatano, K. ( 1998; ). Union of the genera Microbacterium Orla-Jensen and Aureobacterium Collins et al. in a redefined genus Microbacterium. . Int J Syst Bacteriol 48, 739–747.[CrossRef]
    [Google Scholar]
  30. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  31. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  32. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  33. Uchida, K., Kudo, T., Suzuki, K. & Nakase, T. ( 1999; ). A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45, 49–56.[CrossRef]
    [Google Scholar]
  34. Van de Peer, Y. & De Wachter, R. ( 1994; ). treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10, 569–570.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.017699-0
Loading
/content/journal/ijsem/10.1099/ijs.0.017699-0
Loading

Data & Media loading...

Supplements

vol. , part 11, pp. 2634 - 2638

Transmission electron micrograph of negatively stained cells of strain MNA2 grown on LB agar at 28°C for 72h. Bar, 1µm.

Cellular fatty acid composition of strain MNA2

Summed features represent two or three fatty acids that cannot be separated by the Microbial Identification System. Summed feature 8 consisted of C ω7 and/or C ω6 .



IMAGE

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error