1887

Abstract

A spore-forming anaerobic bacterium, designated strain P6, was isolated from the sludge of an up-flow anaerobic sludge blanket reactor treating brewery wastewater. Cells were Gram-positive, oval and 0.6–0.9 μm by 1.2–1.8 μm in size. Growth was observed at 20–42 °C and at pH 5.0–7.5. It fermented several hexoses, polysaccharides and alcohols. Sucrose and aesculin could also be fermented. The main end products of fermentation from glucose were acetate, lactate and fumarate; trace CO and H were also produced. The DNA G+C content of strain P6 was 55.6 mol%. The major cellular fatty acids were iso-C, anteiso-C and iso-C 3-OH. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain P6 represented a novel phyletic sublineage in clostridial cluster III, and showed <91 % similarity to the type strains of recognized species in this cluster. Phenotypically, the new isolate was distinguished from its phylogenetic relatives (e.g. , , and ) by producing acid from glucose and its inability to degrade cellulose. On the basis of evidence from this polyphasic study, strain P6 is considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is P6 (=JCM 14006 =AS 1.5064).

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2010-12-01
2019-10-22
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