1887

Abstract

An actinomycete, designated strain GIMN4.002, was isolated from a tomato rhizosphere soil sample in Guangzhou, China. The strain produces white aerial mycelium and dark blue diffusible pigment on Gause's synthetic agar, and microscopic observation revealed that it produces looped chains of spiny spores. Morphological and chemotaxonomic characteristics of the strain are typical of the genus Melanin was produced and antibacterial activity was detected against Gram-positive micro-organisms, such as , and . The 16S rRNA gene sequence of strain GIMN4.002 had highest similarity (99.4 %) to B91; however, DNA–DNA relatedness between strain GIMN4.002 and NBRC 13054 was only 32.17 %. Further, the morphological, physiological and biochemical characteristics of strain GIMN4.002 are distinct from and other species of the genus with which this strain has high 16S rRNA gene sequence similarity (98–99 %). On the basis of the physiological and molecular properties observed, it is proposed that strain GIMN4.002 represents a novel species of the genus , for which the name sp. nov. is proposed, with GIMN4.002 (=CCTCC M 208213 =NRRL B-24802) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.017392-0
2011-03-01
2019-10-13
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/61/3/507.html?itemId=/content/journal/ijsem/10.1099/ijs.0.017392-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  2. Atlas, R. M. ( 1993; ). Handbook of Microbiological Media. Edited by Parks, L. C.. Boca Raton, FL. : CRC Press.
    [Google Scholar]
  3. Bérdy, J. ( 2005; ). Bioactive microbial metabolites. J Antibiot (Tokyo) 58, 1–26.[CrossRef]
    [Google Scholar]
  4. Butte, W. ( 1983; ). Rapid method for the determination of fatty acid profiles from fats and oils using trimethylsulphonium hydroxide for transesterification. J Chromatogr A 261, 142–145.[CrossRef]
    [Google Scholar]
  5. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  6. Cui, X.-L., Mao, P.-H., Zeng, M., Li, W.-J., Zhang, L.-P., Xu, L.-H. & Jiang, C.-L. ( 2001; ). Streptimonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51, 357–363.
    [Google Scholar]
  7. Edwards, U., Rogall, T., Blöcker, H., Emde, M. & Böttger, E. C. ( 1989; ). Isolation and direct complete nucleotide determination of entire genes. Characterization of gene coding for 16S ribosomal RNA. Nucleic Acids Res 17, 7843–7853.[CrossRef]
    [Google Scholar]
  8. Euzéby, J. P. ( 2008; ). List of prokaryotic names with standing in nomenclature. http://www.bacterio.cict.fr.
  9. Ezaki, T., Hashimoto, Y., Takeuchi, N., Yamamoto, H., Liu, S.-L., Miura, H., Matsui, K. & Yabuuchi, E. ( 1988; ). Simple genetic method to identify viridans group streptococci by colorimetric dot hybridization and fluorometric hybridization in microdilution wells. J Clin Microbiol 26, 1708–1713.
    [Google Scholar]
  10. Gause, G. F., Preobrazhenskaya, T. P., Sveshnikova, M. A., Terekhova, L. P. & Maximova, T. S. ( 1983; ). A Guide for the Determination of Actinomycetes. Genera Streptomyces, Streptoverticillium, and Chainia. Moscow: Nauka (in Russian).
  11. Groth, I., Vettermann, R., Shuetze, B., Schumann, P. & Saiz-Jimenez, C. ( 1999; ). Actinomycetes in Karstic caves of northern Spain (Altamira and Tito Bustillo). J Microbiol Methods 36, 115–122.[CrossRef]
    [Google Scholar]
  12. Hasegawa, T., Takizawa, M. & Tanida, S. ( 1983; ). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef]
    [Google Scholar]
  13. Jeanmougin, F., Thompson, J. D., Gouy, M., Higgins, D. G. & Gibson, T. J. ( 1998; ). Multiple sequence alignment with Clustal X. Trends Biochem Sci 23, 403–405.[CrossRef]
    [Google Scholar]
  14. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  15. Kornerup, A. & Wanscher, J. H. ( 1978; ). Methuen Handbook of Colour, 3rd edn. London. : Eyre Methuen.
    [Google Scholar]
  16. Kroppenstedt, R. M. ( 1985; ). Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society of Applied Bacteriology Technical Series vol. 20), pp. 173–199. Edited by Goodfellow, M. & Minnikin, D. E.. New York. : Academic Press.
    [Google Scholar]
  17. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  18. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  19. Lechevalier, H. A. & Lechevalier, M. P. ( 1970; ). A critical evaluation of the genera of aerobic actinomycetes. In The Actinomycetales, pp. 393–405. Edited by Prauser, H.. Jena. : VEB Gustav Fischer.
    [Google Scholar]
  20. Mandel, M. & Marmur, J. ( 1968; ). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B, 195–206.
    [Google Scholar]
  21. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  22. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  23. Sawabe, T., Makino, H., Tatsumi, M., Nakano, K., Tajima, K., Iqbal, M. M., Yumoto, I., Ezura, Y. & Christen, R. ( 1998; ). Pseudoalteromonas bacteriolytica sp. nov., a marine bacterium that is the causative agent of red spot disease of Laminaria japonica. Int J Syst Bacteriol 48, 769–774.[CrossRef]
    [Google Scholar]
  24. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  25. Shirling, E. B. & Gottlieb, D. ( 1968; ). Cooperative description of type cultures of Streptomyces III. Additional species descriptions from first and second studies. Int J Syst Bacteriol 18, 279–392.[CrossRef]
    [Google Scholar]
  26. Shirling, E. B. & Gottlieb, D. ( 1969; ). Cooperative description of type cultures of Streptomyces. IV. Species descriptions from the second, third and fourth studies. Int J Syst Bacteriol 19, 391–512.[CrossRef]
    [Google Scholar]
  27. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  28. Waksman, S. A. & Henrici, A. T. ( 1943; ). The nomenclature and classification of the actinomycetes. J Bacteriol 46, 337–341.
    [Google Scholar]
  29. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  30. Williams, S. T., Goodfellow, M., Alderson, G., Wellington, E. M. H., Sneath, P. H. A. & Sackin, M. J. ( 1983; ). Numerical classification of Streptomyces and related genera. J Gen Microbiol 129, 1743–1813.
    [Google Scholar]
  31. Williams, S. T., Goodfellow, M. & Alderson, G. ( 1989; ). Genus Streptomyces Waksman and Henrici 1943, 339AL. In Bergey's Manual of Systematic Bacteriology, vol. 4, pp. 2452–2492. Edited by Williams, S. T., Sharpe, M. E. & Holt, J. G.. Baltimore. : Williams & Wilkins.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.017392-0
Loading
/content/journal/ijsem/10.1099/ijs.0.017392-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error