1887

Abstract

A yellow-pigmented, Gram-reaction-negative bacterium isolated from a human clinical source was investigated using a polyphasic approach in order to clarify its taxonomic status. Comparative 16S rRNA gene sequence analysis showed that the new isolate constituted a distinct phyletic line within the genus , displaying >2.8 % sequence divergence with recognized species of this genus. The generic assignment was confirmed by chemotaxonomic data which revealed a fatty acid profile consisting of straight-chain saturated, monounsaturated and branched-chain fatty acids of iso-/anteiso-types as well as 3-hydroxylated fatty acids; a menaquinone with six isoprene units (MK-6) as the predominant respiratory quinone and homospermidine as the predominant polyamine. The novel isolate could be distinguished from other members of the genus by a set of distinct biochemical properties. On the basis of phenotypic and phylogenetic evidence, it is proposed that the new isolate represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is IMMIB L-1519 (=DSM 22251=CCUG 57657).

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2010-09-01
2019-12-16
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References

  1. Barrow, G. I. & Feltham, R. K. A. ( 1993; ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press.
  2. Behrendt, U., Ulrich, A., Spröer, C. & Schumann, P. ( 2007; ). Chryseobacterium luteum sp. nov., associated with the phyllosphere of grasses. Int J Syst Evol Microbiol 57, 1881–1885.[CrossRef]
    [Google Scholar]
  3. Bernardet, J.-F., Nakagawa, Y. & Holmes, B. ( 2002; ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef]
    [Google Scholar]
  4. Bernardet, J.-F., Vancanneyt, M., Matte-Tailliez, O., Grisez, L., Tailliez, P., Bizet, C., Nowakowski, M., Kerouault, B. & Swings, J. ( 2005; ). Polyphasic study of Chryseobacterium strains isolated from diseased aquatic animals. Syst Appl Microbiol 28, 640–660.[CrossRef]
    [Google Scholar]
  5. Bernardet, J.-F., Hugo, C. & Bruun, B. ( 2006; ). The genera Chryseobacterium and Elizabethkingia. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 7, pp. 638–676. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer.
  6. Busse, H.-J. & Auling, G. ( 1988; ). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. Syst Appl Microbiol 11, 1–8.[CrossRef]
    [Google Scholar]
  7. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  8. de Beer, H., Hugo, C. J., Jooste, P. J., Vancanneyt, M., Coenye, T. & Vandamme, P. ( 2006; ). Chryseobacterium piscium sp. nov., isolated from fish of the South Atlantic Ocean off South Africa. Int J Syst Evol Microbiol 56, 1317–1322.[CrossRef]
    [Google Scholar]
  9. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  10. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  11. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  12. Gordon, R. E. ( 1966; ). Some criteria for the recognition of Nocardia madurae (Vincent) Blanchard. J Gen Microbiol 45, 355–364.[CrossRef]
    [Google Scholar]
  13. Gordon, R. E. ( 1967; ). The taxonomy of soil bacteria. In The Ecology of Soil Bacteria, pp. 293–321. Edited by T. R. G. Gray & B. Parkinson. Liverpool: University Press.
  14. Gordon, R. E. & Mihm, J. M. ( 1957; ). A comparative study of some strains received as nocardiae. J Bacteriol 73, 15–27.
    [Google Scholar]
  15. Herzog, P., Winkler, I., Wolking, D., Kämpfer, P. & Lipski, A. ( 2008; ). Chryseobacterium ureilyticum sp. nov., Chryseobacterium gambrini sp. nov., Chryseobacterium pallidum sp. nov. and Chryseobacterium molle sp. nov., isolated from beer-bottling plants. Int J Syst Evol Microbiol 58, 26–33.[CrossRef]
    [Google Scholar]
  16. Hwang, M.-N. & Ederer, G. M. ( 1975; ). Rapid hippurate hydrolysis method for presumptive identification of Group B streptococci. J Clin Microbiol 1, 114–115.
    [Google Scholar]
  17. Kämpfer, P., Lodders, N., Vaneechoutte, M. & Wauters, G. ( 2009a; ). Transfer of Sejongia antarctica, Sejongia jeonii and Sejongia marina to the genus Chryseobacterium as Chryseobacterium antarcticum comb. nov., Chryseobacterium jeonii comb. nov., and Chryseobacterium marinum comb. nov. Int J Syst Evol Microbiol 59, 2238–2240.[CrossRef]
    [Google Scholar]
  18. Kämpfer, P., Vaneechoutte, M., Lodders, N., De Baere, T., Avesani, V., Janssens, M., Busse, H.-J. & Wauters, G. ( 2009b; ). Description of Chryseobacterium anthropi sp. nov., to accommodate clinical isolates biochemically similar to Kaistella koreensis and Chryseobacterium haifense, proposal to reclassify Kaistella koreensis as Chryseobacterium koreense comb. nov. and emended description of the genus Chryseobacterium. Int J Syst Evol Microbiol 59, 2421–2428.[CrossRef]
    [Google Scholar]
  19. Kim, K. K., Bae, H. S., Schumann, P. & Lee, S. T. ( 2005; ). Chryseobacterium daecheongense sp. nov., isolated from freshwater lake sediment. Int J Syst Evol Microbiol 55, 133–138.[CrossRef]
    [Google Scholar]
  20. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  21. Minnikin, D. E., Hutchinson, I. G., Caldicott, A. B. & Goodfellow, M. ( 1980; ). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr A 188, 221–223.[CrossRef]
    [Google Scholar]
  22. Park, S. C., Kim, M. S., Baik, K. S., Kim, E. M., Rhee, M. S. & Seong, C. N. ( 2008; ). Chryseobacterium aquifrigidense sp. nov., isolated from a water-cooling system. Int J Syst Evol Microbiol 58, 607–611.[CrossRef]
    [Google Scholar]
  23. Quan, Z.-X., Kim, K. K., Kim, M.-K., Jin, L. & Lee, S. T. ( 2007; ). Chryseobacterium caeni sp. nov., isolated from bioreactor sludge. Int J Syst Evol Microbiol 57, 141–145.[CrossRef]
    [Google Scholar]
  24. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E. ( 1996; ). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsiaceae fam. nov. Int J Syst Bacteriol 46, 1028–1096.
    [Google Scholar]
  25. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  26. Segers, P., Mannheim, W., Vancanneyt, M., De Brandt, K., Hinz, K.-H., Kersters, K. & Vandamme, P. ( 1993; ). Riemerella anatipestifer gen. nov., comb. nov., the causative agent of septicemia anserum exsudativa, and its phylogenetic affiliation within the Flavobacterium-Cytophaga rRNA homology group. Int J Syst Bacteriol 43, 768–776.[CrossRef]
    [Google Scholar]
  27. Sierra, G. ( 1957; ). A simple method for the detection of lipolytic activity of micro-organisms and some observations on the influence of the contact between cells and fatty substrates. Antonie van Leeuwenhoek 23, 15–22.[CrossRef]
    [Google Scholar]
  28. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  29. Stackebrandt, E. & Ebers, J. ( 2006; ). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33, 152–155.
    [Google Scholar]
  30. Stolz, A., Busse, H.-J. & Kämpfer, P. ( 2007; ). Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 57, 572–576.[CrossRef]
    [Google Scholar]
  31. Vandamme, P., Bernardet, J. F., Segers, P., Kersters, K. & Holmes, B. ( 1994; ). New perspectives in the classification of the flavobacteria: description of Chryseobacterium gen. nov., Bergeyella gen. nov., and Empedobacter nom. rev. Int J Syst Bacteriol 44, 827–831.[CrossRef]
    [Google Scholar]
  32. Vaneechoutte, M., Kämpfer, P., De Baere, T., Avesani, V., Janssens, M. & Wauters, G. ( 2007; ). Description of Chryseobacterium hominis sp. nov. to accommodate clinical isolates biochemically similar to CDC groups II-h and II-c. Int J Syst Evol Microbiol 57, 2623–2628.[CrossRef]
    [Google Scholar]
  33. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  34. Yassin, A. F. & Hupfer, H. ( 2006; ). Williamsia deligens sp. nov., isolated from human blood. Int J Syst Evol Microbiol 56, 193–197.[CrossRef]
    [Google Scholar]
  35. Yassin, A. F., Haggenei, B., Budzikiewicz, H. & Schaal, K. P. ( 1993; ). Fatty acid and polar lipid composition of the genus Amycolatopsis: application of fast atom bombardment mass spectrometry to structure analysis of underivatized phospholipids. Int J Syst Bacteriol 43, 414–420.[CrossRef]
    [Google Scholar]
  36. Yassin, A. F., Chen, W. M., Hupfer, H., Siering, C., Kroppenstedt, R. M., Arun, A. B., Lai, W. A., Shen, F. T., Rekha, P. D. & Young, C. C. ( 2007; ). Lysobacter defluvii sp. nov., isolated from municipal solid waste. Int J Syst Evol Microbiol 57, 1131–1136.[CrossRef]
    [Google Scholar]
  37. Yi, H., Yoon, H. I. & Chun, J. ( 2005; ). Sejongia antarctica gen. nov., sp. nov. and Sejongia jeonii sp. nov., isolated from the Antarctic. Int J Syst Evol Microbiol 55, 409–416.[CrossRef]
    [Google Scholar]
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vol. , part 9, pp. 1993 - 1998

Two-dimensional TLC of phospholipids of strain IMMIB L-1519 . Abbreviations: PE, phosphatidylethanolamine ; AL, unknown aminophospholipid. Molybdenum blue was used to spray the TLC plate.



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