1887

Abstract

A Gram-stain-positive, aerobic bacterium, designated strain BZ41, was isolated from hydrocarbon-contaminated soil. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain BZ41 was related to members of the genus and showed highest similarity with the type strain of (96.8 %). The morphological, biochemical and chemotaxonomic characteristics of the new isolate were consistent with the description of the genus . The cell-wall peptidoglycan of strain BZ41 was of type B2 and contained the amino acids 2,4-diaminobutyric acid, alanine, glycine and glutamic acid in an approximate molar ratio of 1.8 : 0.7 : 1.1 : 1.0. The predominant cell-wall sugars were galactose, glucose, mannose and rhamnose. Strain BZ41 had MK-12 and MK-11 as major menaquinones and contained anteiso-C and anteiso-C as major fatty acids. The genomic DNA G+C content of strain BZ41 was 69.7 mol%. On the basis of phenotypic characteristics and genotypic analysis, strain BZ41 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BZ41 (=DSM 22275 =CGMCC 1.8984).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.017186-0
2010-10-01
2019-10-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/10/2341.html?itemId=/content/journal/ijsem/10.1099/ijs.0.017186-0&mimeType=html&fmt=ahah

References

  1. Collins, M. D. ( 1985; ). Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow, M. & Minnikin, D. E.. London. : Academic Press.
    [Google Scholar]
  2. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  3. Dorofeeva, L. V., Krausova, V. I., Evtushenko, L. I. & Tiedje, J. M. ( 2003; ). Agromyces albus sp. nov., isolated from a plant (Androsace sp.). Int J Syst Evol Microbiol 53, 1435–1438.[CrossRef]
    [Google Scholar]
  4. Gledhill, W. E. & Casida, L. E., Jr ( 1969; ). Predominant catalase-negative soil bacteria. III. Agromyces, gen. nov., microorganisms intermediary to Actinomyces and Nocardia. Appl Microbiol 18, 340–349.
    [Google Scholar]
  5. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  6. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  7. Jurado, V., Groth, I., Gonzalez, J. M., Laiz, L. & Saiz-Jimenez, C. ( 2005a; ). Agromyces salentinus sp. nov. and Agromyces neolithicus sp. nov. Int J Syst Evol Microbiol 55, 153–157.[CrossRef]
    [Google Scholar]
  8. Jurado, V., Groth, I., Gonzalez, J. M., Laiz, L., Schuetze, B. & Saiz-Jimenez, C. ( 2005b; ). Agromyces italicus sp. nov., Agromyces humatus sp. nov. and Agromyces lapidis sp. nov., isolated from Roman catacombs. Int J Syst Evol Microbiol 55, 871–875.[CrossRef]
    [Google Scholar]
  9. Kates, M. ( 1986; ). Techniques of Lipidology, 2nd edn. Amsterdam. : Elsevier.
    [Google Scholar]
  10. Li, W.-J., Zhang, L.-P., Xu, P., Cui, X.-L., Xu, L.-H., Zhang, Z., Schumann, P., Stackebrandt, E. & Jiang, C.-L. ( 2003; ). Agromyces aurantiacus sp. nov., isolated from a Chinese primeval forest. Int J Syst Evol Microbiol 53, 303–307.[CrossRef]
    [Google Scholar]
  11. MacKenzie, S. L. ( 1987; ). Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70, 151–160.
    [Google Scholar]
  12. Margesin, R., Zacke, G. & Schinner, F. ( 2002; ). Characterization of heterotrophic microorganisms in alpine glacier cryoconite. Arct Antarct Alp Res 34, 88–93.[CrossRef]
    [Google Scholar]
  13. Margesin, R., Gander, S., Zacke, G., Gounot, A. M. & Schinner, F. ( 2003; ). Hydrocarbon degradation and enzyme activities of cold-adapted bacteria and yeasts. Extremophiles 7, 451–458.[CrossRef]
    [Google Scholar]
  14. Reasoner, D. J. & Geldreich, E. E. ( 1985; ). A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 49, 1–7.
    [Google Scholar]
  15. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY. : Cold Spring Harbor Laboratory.
    [Google Scholar]
  16. Sasaki, J., Chijimatsu, M. & Suzuki, K. ( 1998; ). Taxonomic significance of 2,4-diaminobutyric acid isomers in the cell wall peptidoglycan of actinomycetes and reclassification of Clavibacter toxicus as Rathayibacter toxicus comb. nov. Int J Syst Bacteriol 48, 403–410.[CrossRef]
    [Google Scholar]
  17. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  18. Schleifer, K. H. ( 1985; ). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123–156.
    [Google Scholar]
  19. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  20. Sly, L. I., Blackall, L. L., Kraat, P. C., Tian-Shen, T. & Sangkhobol, V. ( 1986; ). The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J Microbiol Methods 5, 139–156.[CrossRef]
    [Google Scholar]
  21. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  22. Takeuchi, M. & Hatano, K. ( 2001; ). Agromyces luteolus sp. nov., Agromyces rhizospherae sp. nov. and Agromyces bracchium sp. nov., from the mangrove rhizosphere. Int J Syst Evol Microbiol 51, 1529–1537.
    [Google Scholar]
  23. Whiton, R. S., Lau, P., Morgan, S. L., Gilbart, J. & Fox, A. ( 1985; ). Modifications in the alditol acetate method for analysis of muramic acid and other neutral and amino sugars by capillary gas chromatography–mass spectrometry with selected ion monitoring. J Chromatogr 347, 109–120.[CrossRef]
    [Google Scholar]
  24. Wu, C., Lu, X., Qin, M., Wang, Y. & Ruan, J. (( 1989; ). ). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16, 176–178.
    [Google Scholar]
  25. Yoon, J.-H., Schumann, P., Kang, S.-J., Park, S. & Oh, T.-K. ( 2008; ). Agromyces terreus sp. nov., isolated from soil. Int J Syst Evol Microbiol 58, 1308–1312.[CrossRef]
    [Google Scholar]
  26. Zgurskaya, H. I., Evtushenko, L. I., Akimov, V. N., Voyevoda, H. V., Dobrovolskaya, T. G., Lysak, L. V. & Kalakoutskii, L. V. ( 1992; ). Emended description of the genus Agromyces and description of Agromyces cerinus subsp. cerinus sp. nov., subsp. nov., Agromyces cerinus subsp. nitratus sp. nov., subsp. nov., Agromyces fucosus subsp. fucosus sp. nov., subsp. nov., and Agromyces fucosus subsp. hippuratus sp. nov., subsp. nov. Int J Syst Bacteriol 42, 635–641.[CrossRef]
    [Google Scholar]
  27. Zhang, D.-C., Wang, H.-X., Cui, H.-L., Yang, Y., Liu, H.-C., Dong, X.-Z. & Zhou, P.-J. ( 2007; ). Cryobacterium psychrotolerans sp. nov., a novel psychrotolerant bacterium isolated from the China No.1 glacier. Int J Syst Evol Microbiol 57, 866–869.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.017186-0
Loading
/content/journal/ijsem/10.1099/ijs.0.017186-0
Loading

Data & Media loading...

Supplements

Two-dimensional TLC (Merck silica gel 60 F aluminium-backed thin-layer plates) of the phospholipids (a) and glycolipids (b) of strain BZ41 . Chloroform/methanol/water (65:25:4) was used in the first direction, followed by chloroform/acetic acid/methanol/water (80:15:12:4) in the second direction. The following spray reagents were used for detection: (a) Zinzadze reagent (Kates, 1986) (for phospholipids); (b) α-naphthol-sulphuric acid reagent (for glycolipids). DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; GL, unknown glycolipid.

IMAGE

Two-dimensional TLC (Merck silica gel 60 F aluminium-backed thin-layer plates) of the phospholipids (a) and glycolipids (b) of strain BZ41 . Chloroform/methanol/water (65:25:4) was used in the first direction, followed by chloroform/acetic acid/methanol/water (80:15:12:4) in the second direction. The following spray reagents were used for detection: (a) Zinzadze reagent (Kates, 1986) (for phospholipids); (b) α-naphthol-sulphuric acid reagent (for glycolipids). DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; GL, unknown glycolipid.

IMAGE

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error