1887

Abstract

A novel, aerobic, Gram-positive actinomycete strain, designated XMU15, was isolated from an ocean sediment collected from Zhaoan Bay in the East China Sea and was subjected to a polyphasic approach to determine its taxonomic position. The isolate grew optimally at 28 °C and at pH 7.0 in the presence of 3 % (w/v) NaCl on ISP medium 2. Gelatin liquefaction, milk coagulation and nitrate reduction were positive. Cellulose and starch hydrolysis, hydrogen sulfide and melanin production, and catalase, urease and oxidase activities were negative. The predominant menaquinone of the isolate was MK-9 (H), and -diaminopimelic acid was the diagnostic amino acid in the cell wall. The phospholipids of the isolate comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and a minor amount of phosphatidylethanolamine. The major fatty acids of the strain were iso-C (26.36 %), C 6 (16.80 %), C (16.2 %), C (8.90 %), C 8 (7.69 %) and iso-C H (5.95 %). The DNA G+C content of the genomic DNA was 68.1 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that the isolate fell within the evolutionary radiation encompassed by the genus and showed the highest 16S rRNA gene sequence similarity (96.7 %) to DSM 44391. Based on the results of 16S rRNA gene sequence analysis and phenotypic and genotypic characterization, strain XMU15 (=KCTC 19701 =CCTCC AA 209048) represents a novel species of the genus , for which the name sp. nov. is proposed.

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2010-08-01
2019-10-15
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Scanning electron micrograph of the spores of strain XMU15 grown on yeast extract/malt extract agar (ISP 2) at 28°C for 14 days. Bar, 5 µm.

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