1887

Abstract

A beige-pigmented bacterium (strain CCUG 53761A) was isolated from human blood from an 85-year-old man in Göteborg, Sweden. Comparative analysis of 16S rRNA gene sequences showed that this bacterium displayed <95 % similarity to all described species of the genera of the family . It grouped within the radiation of the genus , but showed only 93.0–94.8 % similarity to type strains of members of this genus ( subsp. , 94.8 %; subsp. , 94.2 %; subsp. , 93.4 %). This discrimination was supported by chemotaxonomic differences. The polyamine pattern consisted of the predominant compound putrescine, moderate amounts of spermidine and minor to trace amounts of spermine and cadaverine; 2-hydroxyputrescine was not detectable. The quinone system was ubiquinone Q-8 with minor amounts of Q-7. The polar lipid profile was composed of the major lipids diphosphatidylglycerol and phosphatidylethanolamine and moderate amounts of phosphatidylglycerol and an unknown phospholipid; minor lipids were also detected. The fatty acid profile, with large amounts of C and C cyclo and the absence of C 2-OH as hydroxylated fatty acid, also differed significantly from those reported for species. On the basis of these data, it is proposed that strain CCUG 53761A represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is CCUG 53761A =CCM 7698.

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2010-07-01
2020-01-21
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References

  1. Altenburger, P., Busse, H.-J., Kämpfer, P., Lubitz, W. & Makristathis, A. ( 1996; ). Classification of bacteria isolated from a medieval wall painting. J Biotechnol 47, 39–52.[CrossRef]
    [Google Scholar]
  2. Auling, G., Busse, H.-J., Pilz, F., Webb, L., Kneifel, H. & Claus, D. ( 1991; ). Rapid differentiation, by polyamine analysis, of Xanthomonas strains from phytopathogenic pseudomonads and other members of the class Proteobacteria interacting with plants. Int J Syst Bacteriol 41, 223–228.[CrossRef]
    [Google Scholar]
  3. Blümel, S., Mark, B., Busse, H.-J., Kämpfer, P. & Stolz, A. ( 2001; ). Pigmentiphaga kullae gen. nov., sp. nov., a novel member of the family Alcaligenaceae with the ability to decolorize azo dyes aerobically. Int J Syst Evol Microbiol 51, 1867–1871.[CrossRef]
    [Google Scholar]
  4. Busse, J. & Auling, G. ( 1988; ). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. Syst Appl Microbiol 11, 1–8.[CrossRef]
    [Google Scholar]
  5. Busse, H.-J., El-Banna, T., Oyaizu, H. & Auling, G. ( 1992; ). Identification of xenobiotic-degrading isolates from the beta subclass of the Proteobacteria by a polyphasic approach including 16S rRNA partial sequencing. Int J Syst Bacteriol 42, 19–26.[CrossRef]
    [Google Scholar]
  6. Coenye, T., Vancanneyt, M., Falsen, E., Swings, J. & Vandamme, P. ( 2003a; ). Achromobacter insolitus sp. nov. and Achromobacter spanius sp. nov., two novel species isolated from human clinical samples. Int J Syst Evol Microbiol 53, 1819–1824.[CrossRef]
    [Google Scholar]
  7. Coenye, T., Vancanneyt, M., Cnockaert, M. C., Falsen, E., Swings, J. & Vandamme, P. ( 2003b; ). Kerstersia gyiorum gen. nov., sp. nov., a novel Alcaligenes faecalis-like organism isolated from human clinical samples, and reclassification of Alcaligenes hominis Rüger and Tan 1983 as Achromobacter hominis comb. nov. Int J Syst Evol Microbiol 53, 1825–1831.[CrossRef]
    [Google Scholar]
  8. Coenye, T., Vanlaere, E., Samyn, E., Falsen, E., Larsson, P. & Vandamme, P. ( 2005; ). Advenella incenata gen. nov., sp. nov., a novel member of the Alcaligenaceae, isolated from various clinical samples. Int J Syst Evol Microbiol 55, 251–256.[CrossRef]
    [Google Scholar]
  9. De Ley, J., Segers, P., Kersters, K., Mannheim, W. & Lievens, A. ( 1986; ). Intra- and intergeneric similarities of the Bordetella ribosomal ribonucleic acid cistrons: proposal for a new family, Alcaligenaceae. Int J Syst Bacteriol 36, 405–414.[CrossRef]
    [Google Scholar]
  10. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  11. Ghosh, W., Badchi, A., Manda, S., Dam, B. & Roy, P. ( 2005; ). Tetrathiobacter kashmirensis gen. nov., sp. nov., a novel mesophilic, neutrophilic, tetrathionate-oxidizing, facultatively chemolithotrophic betaproteobacterium isolated from soil from a temperate orchard in Jammu and Kashmir, India. Int J Syst Evol Microbiol 55, 1779–1787.[CrossRef]
    [Google Scholar]
  12. Hamana, K. & Takeuchi, M. ( 1998; ). Polyamine profiles as chemotaxonomic markers within alpha, beta, gamma, delta, and epsilon subclasses of class Proteobacteria: distribution of 2-hydroxyputrescine and homospermidine. Microbiol Cult Collect 14, 1–14.
    [Google Scholar]
  13. Hamana, K., Saito, T. & Okada, M. ( 2000; ). Polyamine profiles within the beta subclass of the class Proteobacteria: distribution of 2-hydroxyputrescine. Microbiol Cult Collect 16, 63–69.
    [Google Scholar]
  14. Hamana, K., Sato, W., Gouma, K., Yu, J., Ino, Y., Umemura, Y., Mochizuki, C., Takatsuka, K., Kigure, Y. & other authors ( 2007; ). Cellular polyamine catalogues of the five classes of the phylum Proteobacteria: distributions of homospermidine within the class Alphaproteobacteria, hydroxyputrescine within the class Betaproteobacteria, norspermidine within the class Gammaproteobacteria, and spermidine within the classes Deltaproteobacteria and Epsilonproteobacteria. Ann Gunma Health Sci 27, 1–16.
    [Google Scholar]
  15. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  16. Kämpfer, P., Steiof, M. & Dott, W. ( 1991; ). Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21, 227–251.[CrossRef]
    [Google Scholar]
  17. Kämpfer, P., Dreyer, U., Neef, A., Dott, W. & Busse, H.-J. ( 2003; ). Chryseobacterium defluvii sp. nov., isolated from wastewater. Int J Syst Evol Microbiol 53, 93–97.[CrossRef]
    [Google Scholar]
  18. Kämpfer, P., Denger, K., Cook, A. M., Lee, S.-T., Jäckel, U., Denner, E. B. M. & Busse, H.-J. ( 2006; ). Castellaniella gen. nov., to accommodate the phylogenetic lineage of Alcaligenes defragrans, and proposal of Castellaniella defragrans gen. nov., comb. nov. and Castellaniella denitrificans sp. nov. Int J Syst Evol Microbiol 56, 815–819.[CrossRef]
    [Google Scholar]
  19. Kirchhof, G., Eckert, B., Stoffels, M., Baldani, J. I., Reis, V. M. & Hartmann, A. ( 2001; ). Herbaspirillum frisingense sp. nov., a new nitrogen-fixing bacterial species that occurs in C4-fibre plants. Int J Syst Evol Microbiol 51, 157–168.
    [Google Scholar]
  20. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  21. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  22. Moreira, C., Rainey, F. A., Nobre, M. F., da Silva, M. T. & da Costa, M. S. ( 2000; ). Tepidimonas ignava gen. nov., sp. nov., a new chemolithoheterotrophic and slightly thermophilic member of the β-Proteobacteria. Int J Syst Evol Microbiol 50, 735–742.[CrossRef]
    [Google Scholar]
  23. Olsen, G. J., Matsuda, H., Hagström, R. & Overbeek, R. ( 1994; ). fastDNAml: a tool for construction of phylogenetic trees of DNA sequences using maximum likelihood. Comput Appl Biosci 10, 41–48.
    [Google Scholar]
  24. Pruesse, E., Quast, C., Knittel, K., Fuchs, B. M., Ludwig, W., Peplies, J. & Glöckner, F. O. ( 2007; ). silva: a comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb. Nucleic Acids Res 35, 7188–7196.[CrossRef]
    [Google Scholar]
  25. Rossau, R., Kersters, K., Falsen, E., Jantzen, E., Segers, P., Union, A., Nehls, L. & De Ley, J. ( 1987; ). Oligella, a new genus including Oligella urethralis comb. nov. (formerly Moraxella urethralis), and Oligella ureolytica sp. nov. (formerly CDC group IVe): relationship to Taylorella equigenitalis and related taxa. Int J Syst Bacteriol 37, 198–210.[CrossRef]
    [Google Scholar]
  26. Stolz, A., Bürger, S., Kuhm, A., Kämpfer, P. & Busse, H.-J. ( 2005; ). Pusillimonas noertemannii gen. nov., sp. nov., a new member of the family Alcaligenaceae that degrades substituted salicylates. Int J Syst Evol Microbiol 55, 1077–1081.[CrossRef]
    [Google Scholar]
  27. Stolz, A., Busse, H.-J. & Kämpfer, P. ( 2007; ). Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 57, 572–576.[CrossRef]
    [Google Scholar]
  28. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  29. Tindall, B. J. ( 1990a; ). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66, 199–202.[CrossRef]
    [Google Scholar]
  30. Tindall, B. J. ( 1990b; ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13, 128–130.[CrossRef]
    [Google Scholar]
  31. Vandamme, P., Segers, P., Ryll, M., Hommez, J., Vancanneyt, M., Coopman, R., De Baere, R., Van de Peer, Y., Kersters, K. & other authors ( 1998; ). Pelistega europaea gen. nov., sp. nov., a bacterium associated with respiratory disease in pigeons: taxonomic structure and phylogenetic allocation. Int J Syst Bacteriol 48, 431–440.[CrossRef]
    [Google Scholar]
  32. Willems, A., Gilhaus, H., Beer, W., Mietke, H., Gelderblom, H. R., Burghardt, B., Voigt, W. & Reissbrodt, R. ( 2002; ). Brackiella oedipodis gen. nov., sp. nov., Gram-negative, oxidase-positive rods that cause endocarditis of cotton-topped tamarin (Saguinus oedipus). Int J Syst Evol Microbiol 52, 179–186.
    [Google Scholar]
  33. Yabuuchi, E., Kawamura, Y., Kosako, Y. & Ezaki, T. ( 1998; ). Emendation of the genus Achromobacter and Achromobacter xylosoxidans (Yabuuchi and Yano) and proposal of Achromobacter ruhlandii (Packer and Vishniac) comb. nov., Achromobacter piechaudii (Kirefjian et al.) comb nov., and Achromobacter xylosoxidans subsp. denitrificans (Rüger and Tan) comb. nov. Microbiol Immunol 42, 429–438.[CrossRef]
    [Google Scholar]
  34. Yokota, A., Akagawa-Matsushita, M., Hiraishi, A., Katayama, Y., Urakami, T. & Yamasato, K. ( 1992; ). Distribution of quinone systems in microorganisms: Gram-negative eubacteria. Bull Jpn Fed Cult Coll 8, 136–171.
    [Google Scholar]
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